Antibacterial and anti-quorum sensing activities of the different solvent extracts of Embelia ruminata

Rambaran, N.; Naidoo, Yougasphree; Mohamed, Farzana; Chenia, Hafizah Y.; Baijnath, Himansu

doi:10.1016/j.sajb.2022.11.014

Cited by 3 publications

(8 citation statements)

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“…For comparison, in a study by Manikandan et al [ 56 ], AgNPs synthesized using the fruit extracts of E. ribes showed intermediate and weak antibacterial inhibitory activity against E. coli and S. aureus , respectively. Additionally, in a previous study [ 34 ], E. ruminata chloroform fruit extracts showed greater inhibitory activity against methicillin-resistant S. aureus ATCC 700698 and vancomycin-resistant Enterococcus faecalis ATCC 51299 than the antibiotic controls ciprofloxacin and gentamicin [ 34 ]. Likewise, the fruit Rt and 80 °C AgNPs, at 100 and 200 µg, showed greater antibacterial activity against S. aureus ATCC 700698 than the antibiotic ciprofloxacin.…”

Section: Resultsmentioning

confidence: 99%

“…The leaves, stem-bark, and fruit of E. ruminata were collected from Reservoir Hills, Durban, KwaZulu-Natal, South Africa (29°48′26.7″ S 30°55′43.9″ E), from February to May 2019 [ 34 ]. The species identity was confirmed using taxonomic keys, and a voucher specimen (Rambaran 1, accession number 18256) was deposited in the Ward Herbarium, University of KwaZulu-Natal, South Africa [ 34 ]. Plant samples were separated, air-dried, pulverized to a fine powder, and stored in airtight bottles [ 34 ].…”

Section: Methodsmentioning

confidence: 99%

“…The species identity was confirmed using taxonomic keys, and a voucher specimen (Rambaran 1, accession number 18256) was deposited in the Ward Herbarium, University of KwaZulu-Natal, South Africa [ 34 ]. Plant samples were separated, air-dried, pulverized to a fine powder, and stored in airtight bottles [ 34 ]. For extraction, 20 g of the powdered plant material was added to 200 mL of deionized water in an Erlenmeyer flask and incubated in a water bath at 60 °C for 30 min.…”

Section: Methodsmentioning

confidence: 99%

“…Antibacterial-susceptibility assays were carried out using Kirby–Bauer disc diffusion with methods based on protocols previously published by Rambaran et al [ 34 ]. Briefly, four Gram-negative and seven Gram-positive microorganisms were grown at 37 °C overnight on Mueller-Hinton (MH) agar plates.…”

Section: Methodsmentioning

confidence: 99%

“…The QSI potential of E. ruminata AgNPs was investigated using the agar-overlay diffusion assay, following a protocol based on previously developed methodologies [ 34 ]. Briefly, C. subtsugae CV017 and C. violaceum ATCC 12472, were used as the indicator organisms [ 40 ].…”

Section: Methodsmentioning

confidence: 99%

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Antibacterial and Anti-Quorum Sensing Properties of Silver Nanoparticles Phytosynthesized Using Embelia ruminata

Rambaran,

Naidoo,

Mohamed

et al. 2024

Plants

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The rise in antibiotic resistance (AR) poses an imminent threat to human health. Nanotechnology, together with mechanisms such as quorum sensing (QS), which relies on communication between bacterial cells, may decrease the selective pressure for AR. Thus, this study aimed to investigate the effectiveness of silver nanoparticles (AgNPs) synthesized at room temperature (Rt) and 80 °C using Embelia ruminata leaf, stem-bark, and fruit extracts as antibacterial and anti-QS agents. The phytosynthesized AgNPs solutions were subjected to various characterization assays and assessed for their antibacterial activities. Quantitative QS assays were performed using Chromobacterium subtsugae CV017 and Chromobacterium violaceum ATCC 12472. Synthesized AgNPs were spherical-to-near-spherical in shape, poly-dispersed, and crystalline, with a size range of 21.06–32.15 nm. Fruit AgNPs showed stronger antibacterial activity than AgNPs from other plant organs against selected bacterial strains. In the QS assays, fruit 80 °C AgNPs demonstrated the most significant violacein inhibition in an assay performed using the short-chain acyl homoserine lactone CV017 biosensor, while the leaf and fruit Rt AgNPs demonstrated the most violacein inhibition in an assay performed using the long-chain acyl homoserine lactone ATCC 12472 biosensor. The investigations carried out in this study lay the groundwork for future innovative research into antibacterial and anti-QS strategies using E. ruminata.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Antibacterial and Anti-Quorum Sensing Properties of Silver Nanoparticles Phytosynthesized Using Embelia ruminata

Rambaran,

Naidoo,

Mohamed

et al. 2024

Plants

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In Vitro Antioxidant and Anticancer Potential of the Vegetative and Reproductive Organs of Embelia ruminata

Rambaran,

Naidoo,

Dwarka

et al. 2024

Journal of Herbs, Spices & Medicinal Plants

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Antidiabetic Activity and HPTLC Analysis of a Classical Indian Herbomineral Formulation – Vidangadilouham

Debnath,

Roy,

Sarkar

et al. 2023

CIS

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Background:: Vidangalilouham (VDL) is an Ayurveda classical Indian herbomineral preparation prescribed for its effective antidiabetic activity. Among the official formulations of Ayurveda, five different compositions are available by the same name. The particular composition used in this study is only marketed but has yet to be evaluated. Aim and Objective:: The study aimed to assess and validate the antihyperglycemic activity of VDL in high-fat and low-dose streptozotocin (STZ)-induced diabetic animal models. Methods:: High-fat diet-induced animal model of hyperglycemia was generated by a single intraperitoneal injection of STZ (35 mg/kg body weight) into the rats fed with high fat for the previous 30 days. Seven days after STZ induction, the hyperglycemic rats were treated with VDL orally at 100 and 200 mg/kg body weight daily for 28 days. The fasting blood glucose levels (FBG) were measured every 7th day during the 28 days of treatment. The glycosylated hemoglobin levels, liver and serum biochemical parameters, and histopathological findings were estimated and compared. Results and Discussion:: VDL-treated animals significantly exhibited reduced FBG levels compared to the diabetic control group. The lipid peroxidation and antioxidant enzyme levels, such as CAT, GSH, and SOD, in the VDL-treated groups, were restored toward normal levels compared to diabetic control groups, and the values were comparable with the standard group (metformin). The critical diabetic marker, i.e., glycosylated hemoglobin levels were significantly (*p<0.05) decreased when treated with VDL. Conclusion:: Improvement in the FBG and the restoration of all other biomarkers observed in this study indicate VDL to have excellent antidiabetic activity with meager side effects, and thus, the findings provide a scientific rationale for its use as an antidiabetic agent.

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Antibacterial and anti-quorum sensing activities of the different solvent extracts of Embelia ruminata

Cited by 3 publications

References 85 publications

Antibacterial and Anti-Quorum Sensing Properties of Silver Nanoparticles Phytosynthesized Using Embelia ruminata

Antibacterial and Anti-Quorum Sensing Properties of Silver Nanoparticles Phytosynthesized Using Embelia ruminata

In Vitro Antioxidant and Anticancer Potential of the Vegetative and Reproductive Organs of Embelia ruminata

Antidiabetic Activity and HPTLC Analysis of a Classical Indian Herbomineral Formulation – Vidangadilouham

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