ANTIBACTERIAL AND ANTIBIOFILM ACTIVITIES OF Swietenia macrophylla King ETHANOLIC EXTRACT AGAINST FOODBORNE PATHOGENS

Abstract

Swietenia macrophylla is known to possess several medicinal uses, however, its antibacterial and antibiofilm activities against foodborne pathogens remain not well investigated. The present work was performed to examine the phytochemical compounds, antibacterial and antibiofilm activities of S. macrophylla ethanolic extract (SMEE) against four foodborne pathogens namely, Salmonella typhimurium ATCC 14028, Escherichia coli ATCC 25922, Shigella sonnei ATCC 33862 and Pseudomonas aeruginosa ATCC 10145. The phytochemical analysis of SMEE was performed using gas chromatography-mass spectrometry while the antibacterial activities of SMEE were determined by minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) assays. On the other hand, the antibiofilm and time-killing activities of SMME were evaluated using a crystal violet assay. The result demonstrated that SMEE contained major phytochemical compounds such as olean-12-ene (27.37%), resorcinol (16.45%), 24-noroleana-3,12-diene (13.4%), and germanicol (11.50%). The MIC values of SMEE ranged from 31.25 to 500 µg/mL, while all the MBC values were found to be greater than 1000 µg/mL. At the 12 h exposure to SMEE, all the biofilms were inhibited by 50% except E. coli. Biofilm inhibitory concentration (BIC50) values of SMEE ranged between 5.19 and 42.47 µg/mL. In conclusion, S. macrophylla is a promising source of natural antibacterial and antibiofilm agents to treat foodborne diseases.

A Preliminary Study of Antibiofilm Activity of Ethyl Acetate Extracts of Actinomycetes Isolated from Bris Soil, Terengganu against <em>Salmonella typhimurium</em>

“…Biofilms were formed in the wells of 96-well microplate as previously reported (Man et al 2022). 100 μl of EA extracts (10% (v/v)-50% (v/v)) and 100 μl of bacterial inoculum (OD600: 0.5) were loaded into microplate wells in triplicates.…”

Multiple Bacterial Strategies to Survive Antibiotic Pressure: A Review

“…Overall, biofilm formation can protect bacteria from antibiotics, making them more resistant and difficult to eliminate, leading to persistent infections. Continuous screening for antibiofilm activity of natural products may shed new light on the control of biofilm formation and antibiotic resistance [94][95][96].…”

Methanolic Extract Of Swietenia macrophylla Exhibits Antibacterial And Antibiofilm Efficacy Against Gram-Positive Pathogens

“…MIC determination was performed using the microtiter broth dilution assay method in a sterile 96well microplate with a flat bottom as previously reported (Man et al, 2022). In general, the SMME was serially diluted in distilled water to obtain 1000 µg/mL, 500 µg/mL, 250 µg/mL, 125 µg/mL, 62.5 µg/mL, and 31.3 µg/mL concentrations respectively.…”

“…jms.mabjournal.com Amran et al, 2023 Malaysian Applied Biology biofilms formed by Gram-negative pathogens (Man et al, 2022). However, its antibacterial activity and efficacy on the biofilm formation by Gram-positive pathogens remain not well investigated.…”