Antibacterial and Antibiofilm Activity of Calcite (CaCO3) Nanoparticles Synthesized from Chicken Eggshell

Abstract

Background: The Calcite Calicium Carbonate Nanoparticles (CaCO3NPs) synthesized from the chicken eggshell (CES), this was done by crushing the shell and sieving. Objective: The aim of the current study is to find a way to benefit from CES residues and the possibility of exploiting them in the production of CaCO3NPs, which is used in many medical applications. Material and methods: A hundred burn wounds samples were collected from several clinical sources. A total of 60 bacterial isolates were obtained from the burn wounds. CaCO3NPs has effective antibacterial activity; the inhibition zone was 12 mm for Acinetobacter baumannii, Proteus mirabilis and Staphylococcus aureus, 13 mm for Escherichia coli, 11 mm for Klebsiella pneumoniae, 14 mm for Burkholderia cepacia, and Pseudomonas aeruginosa. Results: The results showed that the MIC for A. baumannii was 25 µg/ml. The same MIC values were for K. pneumoniae and S. aureus, while B. cepacia, E. coli, P. mirabilis, and P. aeruginosa have the MIC was 6.25 µg/ml. The present study shows the efficiency of CaCO3NPs to prevent the biofilms of Gram negative and Gram-positive isolates. Conclusion: Synthesized CaCO3NPs from the chicken eggshell have biological effectiveness and it can be used in dental fillings and bone repair.

Chemically Modified Nanoparticles for Enhanced Antioxidant and Antimicrobial Properties with Cinnamon Essential Oil

“…Despite having some inhibitory effect, it was relatively low, aligning with our research, as TiO 2 alone does not demonstrate strong antimicrobial efficacy. In contrast, Al-Azzawi et al [47] studied the antimicrobial activity of pure CaCO 3 nanoparticles, achieving sensitivity to inhibition in E. coli bacteria at higher concentrations, with a reported MIC of 200 µg/mL. This result contrasts with our results, where an increase in concentration, coupled with the addition of CEO, did not lead to a significant difference in bacterial inhibition.…”

تأثيرات مكملات مسحوق قشرالبيض على الصفات الغذائيةوالميكروبيولوجيةوالحسية للكوكيز

“…Because of the adaptability of calcium carbonate and collagen in a variety of sectors such as medicine, pharmaceutics, and nutrition (ABBASSI, 2023) Calcite Calicium Carbonate Nanoparticles (CaCO3NPs) were manufactured from chicken eggshell (CES) by crushing and filtering the shell.It is utilized in a variety of medical applications. (Al-Azzawi,& Al-Kalifawi, 2023). Eggshells, which were previously considered waste food, can now be put to eggmilk pudding to create a healthy dish high in calcium to help avoid stunting.…”