Antibacterial and antibiofilm activity of Lactobacillus strains secretome and extraction against Escherichia coli isolated from urinary tract infection

Soltani, Nayemeh; Abbasi, Samane; Baghaeifar, Sevda; Taheri, Elham; Jadid, Mahdieh Farhoudi Sefidan; Emami-Naeini, Parisa; Abolhasani, Kamilia; Aslanshirzadeh, Firouz

doi:10.1016/j.btre.2022.e00760

Cited by 10 publications

(7 citation statements)

References 28 publications

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“…Antimicrobial peptides may be one of the most efficient defenses against contagious diseases. Bacteriocin, organic acids, and hydrogen peroxide produced by LAB have been hypothesized to combat pathogenic microorganisms 30 , 31 . The outcome showed that the five strains that were put to the test would be good candidates for use in food preservation.…”

Section: Resultsmentioning

confidence: 99%

Probiotic properties and antimicrobial evaluation of silymarin-enriched Lactobacillus bacteria isolated from traditional curd

Haghshenas

Kiani

Mansoori

et al. 2023

Sci Rep

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Nowadays, the increasing use of medicinal plants in the treatment and prevention of diseases has attracted the attention of researchers. The aim of this work was to investigate the probiotic properties and antibacterial and antifungal activity of silymarin-enriched Lactobacillus bacteria against several important pathogenic bacteria and also Aspergillus flavus as one of the harmful molds in the food and health industries. For this purpose, 52 g-positive and catalase-negative bacteria were isolated from 60 traditional curd samples from Ilam province. Five of the 52 bacterial strains had more than 90% viability in high bile salt and acidic conditions and were selected for further investigation. The five strains with positive results showed good hydrophobicity (≥ 50.30%), auto-aggregation (≥ 53.70%), coaggregation (≥ 28.20%), and high cholesterol removal ability (from 09.20 to 67.20%) and therefore can be considered potential probiotics. The tested strains displayed acceptable antibacterial and antifungal activity against all 12 pathogenic bacteria and A. flavus. Also, the results of the simultaneous antifungal activity of probiotic strains and silymarin showed that the combination of silymarin and probiotics has a significantly better (P < 0.05) antifungal effect than the control group or the probiotic groups alone. Interestingly, in addition to the Limosilactobacillus fermentum C3 strain, the Limosilactobacillus fermentum C18 and Lactiplantibacillus pentosus C20 strains also had significant inhibitory effects against A. flavus when used with silymarin extract in methanol. Meanwhile, silymarin extract in DMSO and PEG increased the antagonistic activity of all five potential probiotic strains.

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Section: Resultsmentioning

confidence: 99%

Probiotic properties and antimicrobial evaluation of silymarin-enriched Lactobacillus bacteria isolated from traditional curd

Haghshenas

Kiani

Mansoori

et al. 2023

Sci Rep

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“…They play a significant role in maintaining the homeostasis of this environment and the disturbance of which could affect the development of many uropathogens 15 , 39 . Many recent studies focus on the antibacterial and antibiofilm properties of natural microbiota of the urinary tract against the most common UTI pathogens such as: Escherichia coli, which cause approximately 80% of UTIs 40 , 41 , Proteus mirabilis 14 , 42 or Klebsiella pneumoniae 43 , 44 . Additionally, a lot of scientific works concentrate on the practical use of selected Lactobacillus strains to UTIs treatment.…”

Section: Discussionmentioning

confidence: 99%

Antibacterial properties and urease suppression ability of Lactobacillus inhibit the development of infectious urinary stones caused by Proteus mirabilis

Szczerbiec,

Bednarska-Szczepaniak,

Torzewska

2024

Sci Rep

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Infectious urolithiasis is a type of urolithiasis, that is caused by infections of the urinary tract by bacteria producing urease such as Proteus mirabilis. Lactobacillus spp. have an antagonistic effect against many pathogens by secreting molecules, including organic acids. The aim of the study was to determine the impact of Lactobacillus strains isolated from human urine on crystallization of urine components caused by P. mirabilis by measuring bacterial viability (CFU/mL), pH, ammonia release, concentration of crystallized salts and by observing crystals by phase contrast microscopy. Moreover, the effect of lactic acid on the activity of urease was examined by the kinetic method and in silico study. In the presence of selected Lactobacillus strains, the crystallization process was inhibited. The results indicate that one of the mechanisms of this action was the antibacterial effect of Lactobacillus, especially in the presence of L. gasseri, where ten times less P. mirabilis bacteria was observed, compared to the control. It was also demonstrated that lactic acid inhibited urease activity by a competitive mechanism and had a higher binding affinity to the enzyme than urea. These results demonstrate that Lactobacillus and lactic acid have a great impact on the urinary stones development, which in the future may help to support the treatment of this health problem.

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“…The positive and negative controls were the wells devoid of bacteria and extract, respectively. By measuring optical density (OD470 nm), MIC and MBC, representing the lowest concentrations of CSF capable of inhibiting the growth of the pathogen and eradicating all pathogenic bacteria, were determined [ 22 ].…”

Section: Methodsmentioning

confidence: 99%

“…Finally, the optical density (OD) of crystal violet associated with biofilms was measured at a wavelength of 570 nm. The experiments were performed three times, utilizing A. baumannii ATCC 196006 as a positive control and an uninfected medium as a negative control [ 22 ].…”

Section: Methodsmentioning

confidence: 99%

Isolation and Identification of Effective Probiotics on Drug-Resistant Acinetobacter baumannii Strains and Their Biofilms

Abbasi,

Ghasemi,

Ahmadi

et al. 2024

Canadian Journal of Infectious Diseases and Medical Microbiolog

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Introduction. This study aimed to identify, assess, and isolate strong lactobacilli demonstrating broad antibacterial and anti-biofilm activity against drug-resistant strains of Acinetobacter baumannii. Additionally, the mechanism of inhibition of these organisms was to be determined. Methods. Over a 6-month period (from December 2021 to June 2022), 53 clinical A. baumannii strains were collected from clinical samples. Twenty probiotic strains were isolated from local dairy products. Antibacterial activity of Lactobacillus strains’ cell-free supernatant (CFS) was identified using the agar well diffusion method and the microbroth dilution test. Anti-biofilm effect was performed by the microtiter plate assay. The MTT assay was also used to look into the probiotics’ cytotoxic effects on the L929 fibroblast cell line. Results. During the 6-month period, 53 clinical A. baumannii strains were obtained and identified. Out of 20 lactobacillus strains, the CFS of a lactobacillus strain (named L9) showed an inhibitory effect against all A. baumannii strains. Using the broth microdilution method, it was shown that the minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs) of CFS extracts of L9 strains against A. baumannii strains were both ¼ mg/mL. The result of the anti-biofilm showed that the selected probiotic could inhibit biofilm formation. The most common organic acid produced by all Lactobacillus strains, according to the HPLC method, was lactic acid, which was followed by acetic acid. The L929 fibroblast cell line was used in the cytotoxicity assay, which revealed that 100% of the cells in the L929 fibroblast cell line survived treatment with successive doses of CFSs for a full day. Conclusion. The probiotic strain isolated from local yogurt in this study showed potential anti-biofilm and antimicrobial properties against all drug-resistant Acinetobacter isolates. Given the increasing interest in probiotic microorganisms based on their high health benefits, further studies are recommended on the mechanisms of action between probiotics and A. baumannii strains to find new solutions for biological control and treatment of these infections without the use of antibiotics.

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Antibacterial and antibiofilm activity of Lactobacillus strains secretome and extraction against Escherichia coli isolated from urinary tract infection

Cited by 10 publications

References 28 publications

Probiotic properties and antimicrobial evaluation of silymarin-enriched Lactobacillus bacteria isolated from traditional curd

Probiotic properties and antimicrobial evaluation of silymarin-enriched Lactobacillus bacteria isolated from traditional curd

Antibacterial properties and urease suppression ability of Lactobacillus inhibit the development of infectious urinary stones caused by Proteus mirabilis

Isolation and Identification of Effective Probiotics on Drug-Resistant Acinetobacter baumannii Strains and Their Biofilms

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