Antibacterial and Anticancer Activity of Protein Sponges Collected From the Waters of Kapoposang Island of South Sulawesi, Indonesia

Sugrani, Andis; Natsir, Hasnah; Djide, M. Natsir; Ahmad, Ahyar

doi:10.7897/2230-8407.100115

Cited by 3 publications

(1 citation statement)

References 5 publications

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“…The antibacterial activity of the collected proteins and peptides were tested using an agar diffusion method (Sugrani et al, 2019a) on the bacteria S. aureus and E. coli. Medium Muller Hinton Agar was sterilized using an autoclave set to 121°C a pressure of 15 psi.…”

Section: Antibacterial Assaymentioning

confidence: 99%

Two novel antimicrobial and anticancer peptides prediction from Vibrio sp. strain ES25

Sugrani¹,

Ahmad²,

Natsir³

et al. 2020

J App Pharm Sci

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Proteins from microbes are a large source of potential raw material for novel pharmaceuticals. Endophytic bacteria associated with algae associated have been shown to be particularly promising candidates. In a previous study, a crude protein extract was isolated from an endophytic Vibrio sp. strain ES25. The extract was found to have anti-microbial activity. Despite these promising results, further work was needed to characterize both the protein and the bacteria that produce it. In this study, we sought to increase the activity of the previously discovered protein through pepsin digestion as well as provide sequence data to characterize the bacterial isolate. Here, we show promising evidence that the hydrolysate produced (QA1d) was found to have a promising antimicrobial as well as anticancer properties. Through the DNA sequencing, we also provide better taxonomic information on the isolate. Finally using Liquid Chromatography-Mass Spectroscopy/Mass Spectroscopy (LC-MS/MS) and in silico prediction, we identify to novel peptides produced from the bacterial protein that were predicted to have multifunctional activity. These results shown here indicate these peptides are promising candidates for anticancer drug development or antibacterial agents for the food industry.

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Section: Antibacterial Assaymentioning

confidence: 99%

Two novel antimicrobial and anticancer peptides prediction from Vibrio sp. strain ES25

Sugrani¹,

Ahmad²,

Natsir³

et al. 2020

J App Pharm Sci

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Toxicological evaluation and antibacterial activity of crude protein extract from endophytic bacteria associated with Algae Eucheuma spinosum

Sugrani

Ahmad

Djide

et al. 2019

J. Phys.: Conf. Ser.

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This study evaluated the toxicological and antibacterial activity of crude extract of endophytic bacteria’s protein associated with the red algae Eucheuma spinosum which was produced at various incubation times. The external and internal proteins of the potential bacteria were collected on the incubation times which were 18, 24, 30, 36, 42, 48, and 54 hours. The evaluation toxicological of protein used Brine Shrimp Lethality Test (BSLT) method and the antibacterial activity of protein towards E. coli and S. aureus were measured using the diffusion method. Seven bacteria were successful to be isolated from these algae (ES01, ES11, ES21, ES22, ES23, ES24, and ES25), the Isolate ES25 was a genus of Vibrio and its protein had the potential to be produced. External protein levels were higher than internal, so it continued with the next phase. The extracted protein of (PE42) was active towards S. aureus (12.0 mm) while the extracted protein of (PE36) is active toward E. coli (9.0 mm) and also highly toxic to the larvae of Artemia salina Leach (1.596 μg/mL). The external protein of endophytic bacteria ES25 (Vibrio) associated with red alga Eucheuma spinosum was potentially produced in the time of incubation above 36 hours to get the highest toxicity and active antibacterial.

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Antibacterial assay of crude extracts from marine sponge Haliclona fascigera in Badi Island of Spermonde Archipelago against shrimp pathogenic bacteria

Latifah

Tahir

Soekamto

2021

IOP Conf. Ser.: Earth Environ. Sci.

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The marine Sponge Haliclona fascigera, belonging to the genus Haliclona, Family Haliclonidae, Order Haplosclerida, is a source of potentially valuable marine natural products, including diverse bioactive secondary metabolites. These bioactive secondary metabolites could be used as alternative antibiotics for managing pathogenic bacteria in aquaculture. The study aimed to determine the in vitro antibacterial activity of n-hexane, ethyl acetate, and acetone crude extracts against three shrimp pathogenic bacteria and to carry out phytochemical screening. The three crude extracts were produced from H. fascigera collected around Badi Island, Spermonde Archipelago, Indonesia. The crude extracts were screened for their activity against three shrimp pathogenic bacteria: Vibrio harveyi (M-120), Vibrio alginolyticus, and Vibrio parahaemolyticus (T-170). Antibacterial activity assays used the agar diffusion method; the paper discs were impregnated with extract concentrations of 2μg/25μL. Phytochemical screening was carried out using standard protocols to provide supporting data. N-hexane and acetone crude extracts of H. fascigera were able to inhibit Vibrio parahaemolyticus with inhibition zone diameters of 8.07mm and 7.62mm, respectively. The inhibition zone of Ciprofloxacin (positive control) was 10.45 mm. The phytochemical analysis indicated that steroid, terpenoid, and alkaloid compounds were present in H. fascigera. Further studies are needed to reveal the compounds causing the observed antimicrobial effect.

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Antibacterial and Anticancer Activity of Protein Sponges Collected From the Waters of Kapoposang Island of South Sulawesi, Indonesia

Cited by 3 publications

References 5 publications

Two novel antimicrobial and anticancer peptides prediction from Vibrio sp. strain ES25

Two novel antimicrobial and anticancer peptides prediction from Vibrio sp. strain ES25

Toxicological evaluation and antibacterial activity of crude protein extract from endophytic bacteria associated with Algae Eucheuma spinosum

Antibacterial assay of crude extracts from marine sponge Haliclona fascigera in Badi Island of Spermonde Archipelago against shrimp pathogenic bacteria

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