Antibacterial and anticancer activity of extracellular synthesized silver nanoparticles from marine Streptomyces rochei MHM13

Abd-Elnaby, Hanan; Abo-Elala, Gehan M.; Abdel-Raouf, Usama M.; Hamed, Moaz M.

doi:10.1016/j.ejar.2016.05.004

Cited by 151 publications

(68 citation statements)

References 27 publications

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“…The diffractogram contained weak diffraction peaks, which could be attributed to the bio-organic molecules on the surface of the silver nanoparticles [ 58 ]. In agreement with our results, Abd-Elnaby et al [ 59 ] reported the extracellular synthesis of silver nanoparticles from marine Streptomyces rochei MHM13 with the particle size range of 22–85 nm.…”

Section: Resultssupporting

confidence: 93%

Bactericidal and In-Vitro Cytotoxic Efficacy of Silver Nanoparticles (Ag-NPs) Fabricated by Endophytic Actinomycetes and Their Use as Coating for the Textile Fabrics

et al. 2020

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An endophytic strain of Streptomyces antimycoticus L-1 was isolated from healthy medicinal plant leaves of Mentha longifolia L. and used for the green synthesis of silver nanoparticles (Ag-NPs), through the use of secreted enzymes and proteins. UV–vis spectroscopy, Fourier-transform infrared (FT-IR), transmission electron microscopy (TEM), X-ray diffraction (XRD), and dynamic light scattering (DLS) analyses of the Ag-NPs were carried out. The XRD, TEM, and FT-IR analysis results demonstrated the successful biosynthesis of crystalline, spherical Ag-NPs with a particle size of 13–40 nm. Further, the stability of the Ag-NPs was assessed by detecting the surface Plasmon resonance (SPR) at 415 nm for one month or by measuring the NPs surface charge (−19.2 mV) by zeta potential analysis (ζ). The green-synthesized Ag-NPs exhibited broad-spectrum antibacterial activity at different concentrations (6.25–100 ppm) against the pathogens Staphylococcus aureus, Bacillus subtilis Pseudomonas aeruginosa, Escherichia coli, and Salmonella typhimurium with a clear inhibition zone ranging from (9.5 ± 0.4) nm to (21.7 ± 1.0) mm. Furthermore, the green-synthesized Ag-NPs displayed high efficacy against the Caco-2 cancerous cell line (the half maximal inhibitory concentration (IC50) = 5.7 ± 0.2 ppm). With respect to antibacterial and in-vitro cytotoxicity analyses, the Ag-NPs concentration of 100 ppm was selected as a safe dose for loading onto cotton fabrics. The scanning electron microscopy connected with energy-dispersive X-ray spectroscopy (SEM-EDX) for the nano-finished fabrics showed the distribution of Ag-NPs as 2% of the total fabric elements. Moreover, the nano-finished fabrics exhibited more activity against pathogenic Gram-positive and Gram-negative bacteria, even after 10 washing cycles, indicating the stability of the treated fabrics.

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Section: Resultssupporting

confidence: 93%

Bactericidal and In-Vitro Cytotoxic Efficacy of Silver Nanoparticles (Ag-NPs) Fabricated by Endophytic Actinomycetes and Their Use as Coating for the Textile Fabrics

et al. 2020

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“…Abd-Elnaby et al (2016) reported an excellent antibacterial activity against Gram-negative and Gram-positive bacterial pathogens by microbiologically synthesized AgNPs. Mostafa et al (2015) report activity of chemically synthesized silver nanoparticles against P. aeruginosa , S. aureus , Streptococcus pyogenes , and S. typhi.…”

Section: Discussionmentioning

confidence: 99%

Efficiency of Biosynthesized Silver and Zinc Nanoparticles Against Multi-Drug Resistant Pathogens

Punjabi

Mehta

Chavan³

et al. 2018

Front. Microbiol.

100

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Biosynthesis of metallic nanoparticles has acquired particular attention due to its economic feasibility, low toxicity, and simplicity of the process. In this study, extracellular synthesis of silver and zinc nanoparticle was carried out by Pseudomonas hibiscicola isolated from the effluent of an electroplating industry in Mumbai. Characterization studies revealed synthesis of 40 and 60 nm nanoparticles of silver (AgNP) and zinc (ZnNP), respectively, with distinct morphology as observed in TEM and its crystalline nature confirmed by XRD. DLS, zeta potential, NTA, and FTIR studies further characterized nanoparticles giving data about its size, stability, and functional groups. Considering the toxicity of nanoparticles the evaluation of antimicrobial activity was studied in the range of non-toxic concentration for normal cell lines. Silver nanoparticles were found to be the most effective antimicrobial against all tested strains and drug-resistant clinical isolates of MRSA, VRE, ESBL, MDR, Pseudomonas aeruginosa with MIC in the range of 1.25–5 mg/ml. Zinc nanoparticles were found to be specifically active against Gram-positive bacteria like Staphylococcus aureus including its drug-resistant variant MRSA. Both AgNP and ZnNP were found to be effective against Mycobacterium tuberculosis and its MDR strain with MIC of 1.25 mg/ml. The synergistic action of nanoparticles assessed in combination with a common antibiotic gentamicin (590 μg/mg) used for the treatment of various bacterial infections by Checker board assay. Silver nanoparticles profoundly exhibited synergistic antimicrobial activity against drug-resistant strains of MRSA, ESBL, VRE, and MDR P. aeruginosa while ZnNP were found to give synergism with gentamicin only against MRSA. The MRSA, ESBL, and P. aeruginosa strains exhibited MIC of 2.5 mg/ml except VRE which was 10 mg/ml for both AgNPs and ZnNPs. These results prove the great antimicrobial potential of AgNP and ZnNP against drug-resistant strains of community and hospital-acquired infections and opens a new arena of antimicrobials for treatment, supplementary prophylaxis, and prevention therapy.

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“…The absorption peak at 3432 cm -1 is assigned to N-H stretching vibration [34], whereas the band at 2925 cm -1 is associated with the C-H stretching vibration-asymmetric [35,36]. The peak at 1385 cm -1 is corresponding to C-H symmetric deformation vibration [37] while the one at 1033 cm -1 is assigned to C-O stretching [38]. The slight shift and reduced intensity of the cinnamon mediated AgNPs spectrum are attributed to the involvement of the cinnamon functional groups in the AgNPs bioreduction process.…”

Section: Physicochemical Characterizationmentioning

confidence: 99%

Synthesis and characterization of nontoxic silver nano-particles with preferential bactericidal activity

2019

Biointerface Res Appl Chem

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The massive prophylactically or remedially application of antibiotics without proper medical indications lead to severe problems of bacterial resistance. Nanoparticles (NPs) are increasingly applied to target bacterial infections as an antibiotic alternative. Green synthesis of silver nanoparticles (AgNPs), with controlled morphology, within cinnamon extract using microwave irradiation was carried out. The influence of the pH on the synthesized AgNPs was assessed. Physicochemical characterizations were followed through UV, FTIR, FESEM, HRTEM, XPS and XRD analyses. Bactericidal activity and in-vitro cytotoxicity of the achieved AgNPs were investigated. Results proved the successful synthesis of spherical AgNPs with an average size of 15 nm. The AgNPs safety was verified through cytotoxicity test against skin fibroblast normal cells. The obtained AgNPs exhibited promising bactericidal activities against the screening of several Gram+ve and Gram-ve bacteria. Therefore, it can be successfully applied in the biomedical fields for hard and soft tissue remedies.

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Antibacterial and anticancer activity of extracellular synthesized silver nanoparticles from marine Streptomyces rochei MHM13

Cited by 151 publications

References 27 publications

Bactericidal and In-Vitro Cytotoxic Efficacy of Silver Nanoparticles (Ag-NPs) Fabricated by Endophytic Actinomycetes and Their Use as Coating for the Textile Fabrics

Bactericidal and In-Vitro Cytotoxic Efficacy of Silver Nanoparticles (Ag-NPs) Fabricated by Endophytic Actinomycetes and Their Use as Coating for the Textile Fabrics

Efficiency of Biosynthesized Silver and Zinc Nanoparticles Against Multi-Drug Resistant Pathogens

Synthesis and characterization of nontoxic silver nano-particles with preferential bactericidal activity

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