This study aims to determine the antimicrobial and antioxidant activities of essential oil components and methanol extracts of Fumaria asepala Boiss. (Akşahtere) and Fumaria schleicheri Soy.-Will. subsp. microcarpa Hausskn. (Şahtere) species grown in Turkey. While essential oils were isolated by the hydrodistillation method, the analysis of these oils was determined by GC/MS device. According to the results obtained, the main component of the essential oil of F. asepala is Phytol (20.74%), followed by Thymol (20.42%), Benzyl Benzoate (15.89%), and Hexahydrofarnesyl acetone (12.92%); It was determined that the main component of the essential oil of F. schleicheri subsp. microcarpa is Benzyl Benzoate (29.07%), followed by Hexahydrofarnesyl acetone (19.72%), n-Hexadecanoic acid (11.40%) and Phytol (10.04%). The disk diffusion method was used to determine its antimicrobial effects. The above-ground parts of F. schleicheri subsp. microcarpa showed the best antimicrobial effect against K. pneumoniae-ATCC 700603 (25mm), while F. asepala showed the best antimicrobial effect against S. aureus-ATCC 25923 (24mm) and K. pneumoniae (24mm). To determine their antioxidant effects, total antioxidant level (TAS) and total oxidant level (TOS) were determined. It was determined that the TAS value of the methanol extract of F. schleicheri subsp. microcarpa at a concentration of 1 mg/ml was 2.8314 mmol, and the TOS value of F. asepala was 3.1610 mmol at the same concentration. It was determined that the total oxidant levels in both species were high.