Antibacterial and Antioxidant Activity of Biosynthesized Silver Nanoparticles From Ulmus Wallichiana Planch. Leaf Extract

Abstract

Eco-friendly synthesis of metal nanoparticles using plant extract plays a vital role in nanotechnology. This study aimed to produce silver nanoparticles (SNPs) in an eco-friendly manner using leaf extract of Ulmus wallichiana Planch. When the leaf extract was treated with AgNO 3 , the colour changed from yellowish to dark brown, indicated the formation of SNPs. The obtaining SNPs were predominantly spherical, crystalline and a mean size of 54 nm. FTIR spectrum evidenced that plant's biomolecules acted as reducing agent. The antibacterial activity of SNPs was assessed against Gram positive (Staphyllococcus aureus) and Gram negative (Escherichia coli, Pseudomonas aeruginosa and Klebsiella pneumoniae) bacteria and highest activity was found to be exerted at 1 mg/mL SNPs against P. aeruginosa (17.63 mm). They also showed profound antioxidant activity against DPPH. Ulmus mediated synthesis of SNPs is eco-friendly, non-toxic and cost effective with good antibacterial and antioxidant effect and can be used in biomedical applications. Rezumat Sinteza ecologică a nanoparticulelor metalice folosind extracte de plante joacă un rol vital în industria nanotehnologiilor. Acest studiu a urmărit biosinteza nanoparticulelor de argint (SNP) într-un mod ecologic, folosind extractul foliar de Ulmus wallichiana Planch. După tratarea extractului cu AgNO 3 , culoarea s-a modificat de la galben la maro închis, indicând formarea nanoparticulelor. SNP-urile obținute au fost predominant sferice, cristaline și au prezentat dimensiunea medie de 54 nm. Spectrul FTIR a evidențiat faptul că biomoleculele din extract au acționat ca agent reducător. Activitatea antibacteriană a nanoparticulelor a fost evaluată asupra bacteriilor Gram pozitive (Staphyllococcus aureus) și Gram negative (Escherichia coli, Pseudomonas aeruginosa și Klebsiella pneumoniae), iar cea mai mare activitate s-a înregistrat pentru doza de 1 mg/mL SNP asupra P. aeruginosa (17,63 mm). De asemenea, au prezentat o activitate antioxidantă pronunțată în testul DPPH. Sinteza mediată de Ulmus a nanoparticulelor de argint este ecologică, non-toxică și rentabilă, cu efect antibacterian și antioxidant, având potențiale aplicații biomedicale.

Synthesis and Antioxidant Activity of Silver Nanoparticles Using the Odontonema strictum Leaf Extract

“…In fact, the capping of silver with phytochemicals of plant extract is assumed to be responsible for antioxidant activity exhibited by AgNPs. Many studies showed that AgNPs capped by aqueous leaf extract exhibited higher antioxidant potential than the leaf extracts [ 38 , 39 ].…”

Complexities underlying the breeding and deployment of Dutch elm disease resistant elms

“…1c, d). The diversity of products and services derived from elms e.g., ecosystem protection, ornamental and landscape value, shade, timber and wood materials, biomass, nanomaterials (Heybroek 1993;Richens 1983;Martín-Sampedro et al 2019;Mehmood 2019;Jiménez-López et al 2020) are good reasons to promote the recovery of the elms. Current restoration initiatives use knowledge, techniques and plant materials developed by several different breeding programs and research groups (Smalley and Guries 1993;Mittempergher and Santini 2004;Pecori et al 2017).…”