Antibacterial and Antioxidant Activity of Mycelial Extracts of Different Pleurotus Species

Abstract

Mushrooms could be used as a potential means of producing natural antioxidants and antimicrobials. To obtain fungal biomass in submerged culture is an easy and rapid method. For this reason, biomasses of Pleurotus species which grown on liquid media were used to prepare hot water extracts and their antioxidant and antibacterial properties were determined. The highest total phenolic content was determined in P. ostreatus extract (9.14 mg.g-1 dry weight of extract) whereas Pleurotus sajor-caju gave highest reading of total flavonoid content (3.10 mg.g-1 dry weight of extract). In the scavenging effect of DPPH radical test, P. sajor-caju showed the highest activity potential (69.67%). Mycelia extracts from Pleurotus species showed the antibacterial activity against the Gram negative and Gram positive bacteria (plant and human pathogens). Based on the results obtained, each extract from the five species Pleurotus (P. florida, P. citrinopileatus, P. sajor-caju, P. ostreatus and P. eryngii) showed antioxidant and antibacterial properties, and could be used in the formulation of nutraceuticals. Furthermore, the results presented in this work demonstrated that extracts were capable of inhibiting the in vitro growth of Helicobacter pylori.

Determination of Bioactive Properties of Alcohol Extracts of Types of Pleurotus sajor-caju and Pleurotus ostreatus

“…Fungi themselves and extracts have antimicrobial effect. It has been reported to have higher activity, especially against Gram-positive bacteria (Reis, 2017;Ozdal et al 2019). In our study, Pleurotus and Lentiudes extracts were found their antibacterial effect on the bacterial species (P. aeuriginosa, S. aureus, E. coli, B. cereus, B. thuringiensis C. diphteria, K. oxycota, S. marcescens and E. faecalis).…”

“…ostreatus and P. sajor-caju were grown at 25 ℃ for 7 days on Potato Dextrose Agar (PDA). Submerged fermentation was achieved in 250 mL Erlenmeyer flasks, containing 100 mL of liquid medium (Ozdal et al 2019). Each flask was inoculated with one 10 mm agar plugs.…”

“…Fungi with high natural and nutritious properties are rich in antioxidant and antimicrobial substances. Both the fruiting bodies and mycelia are high important structures due to biological and pharmacological activities (Vamanu 2012;Ozdal et al 2019).…”

Antimicrobial activity of the edible mushroom Pleurotus eryngii (DC.) Quél grown in liquid medium

“…Although the bacteria tested were resistant in this study, the inhibition of P. aeruginosa using biomass extracted from P. eryngii, P. sajor-caju, P citrinopileatus, P. ostreatus and P. florida in hot water has been mentioned in the literature, and presented an IH which was equivalent to 8.8, 9.0, 8.1, 9.4 and 9.7 mm, respectively (Özdal, Gülmez, Gür-Özdal, & Algur, 2019). Similarly, Fasoranti, Ogidi, and Oyetayo (2018) verified the sensitivity of P. aeruginosa (IH= 14 and 15 mm), S. aureus (IH= 13.7 and 15.7 mm) and E. coli (IH= 15.7 and 14 mm), in relation to the mushrooms P. pulmonarius and P. ostreatus.…”

Biochemical Evaluation of Agaricus and Pleurotus Strains in Batch Cultures for Production Optimization of Valuable Metabolites

“…Similarly, Liu and Wang [ 63 ] used a mixture of glucose and corn extract as a source of glucose and wheat bran and yeast extract as a source of nitrogen in a 30 L bioreactor with an agitated rate of 160 rpm, and a biomass value of 11.30 g/L was then recorded. In experiments with five species of Pleurotus grown for 15 days in a medium containing 20 g/L glucose, 2 g/L peptone and 3 g/L yeast extract, P. eryngii produced 6.1 g/L of biomass, whereas P. ostreatus produced 11.2 g/L [ 64 ]. Kim et al [ 27 ] cultured P. ostreatus in conditions similar to the present study and observed smaller values of produced biomass on the 5th, 10th and 15th culture days—1.07, 4.6 and 7.35 g/L, respectively.…”