Antibacterial and Antioxidant Studies of Green Synthesized Silver Nanoparticles using Azadirachta indica (Neem) Leaf Extract

Regmi, Bishow; Binadi, Tirtha Raj; Jha, Sarb Narayan; Chaudhary, Rajib Kumar; Poudel, Bhoj Raj; Gautam, Surendra Kumar

doi:10.3126/ijasbt.v9i3.39069

Cited by 5 publications

(1 citation statement)

References 17 publications

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“…In the present study, the calculated crystallite size was found to be 9.6 nm was calculated by using the above equation, as shown in Table 3. The calculated crystallite value of thus obtained differed from the studies conducted previously 34,35 .…”

Section: Table 2: Antibiotic Sensitivity Test Of Microbes Isolated Fr...contrasting

confidence: 76%

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2023

IJPSR

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The aim of this study was to evaluate the antimicrobial and antibiofilm activity of silver nanoparticles of Azadirachta indica (AISNPs) against the pathogens isolated from effluent. Green synthesis approach was opted for the synthesis of the synthesis of AISNPs by using the ethanolic extract of Azadirachta indica which was added to 1mM solution of AgNO 3 . These synthesized nanoparticles were molecularly characterized by UV-Visible spectrophotometer, TEM, Zeta Sizer, Zeta Potential, XRD and FTIR respectively. Antimicrobial activity of the AISNPs was carried by well diffusion method on Muller Hinton Agar against biofilm-producing multi-drug resistant effluent pathogens, which were identified as E. coli, P. aeruginosa, S. aureus and K. pneumoniae. The zone of inhibition of AISNPs observed in case of E. coli was 16.08 mm, 18.65 mm against S. aureus, 10.46 mm against P. aeruginosa and 15.34 mm against K. pneumoniae. Antibiofilm activity of AISNPs demonstrated the decline of biofilm producing activity by 76% against P. aeruginosa, 74% against S. aureus, 60% against K. pneumoniae and 59% against E. coli respectively, indicating the strong antibiofilm properties of the AISNPs. It can be concluded from the present study that green synthesized AISNPs can be used as an alternate approach to combat the multiple drugresistant biofilm producing pathogens isolated from effluent. INTRODUCTION:Environment is a multifactorial component, comprising of the natural resources like air, soil and water as essential ingredients and each of these units are directly associated with humans and all the living population found on this planet. Amongst these environmental components utilized by the humans, water is one of the most vital sources.

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Section: Table 2: Antibiotic Sensitivity Test Of Microbes Isolated Fr...contrasting

confidence: 76%

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2023

IJPSR

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Silver nanoparticles reinforced polyethersulfone composite for sustainable application

Owoyemi,

Adewuyi,

Oladele

et al. 2024

Discov Polym

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Polyethersulfone composites reinforced with biologically synthesized silver nanoparticles (AgNPs) were fabricated via compression molding at 30 GPa and 250 °C, with nanoparticle concentrations ranging from 0.5 to 3.0 wt.%. Neem leaf extract served as the bioreducing agent in AgNP synthesis. Characterization using transmission electron microscopy, scanning electron microscopy with energy dispersive X-ray spectroscopy, and X-ray diffraction confirmed the formation of spherical AgNPs with an average size of approximately 21 nm and a face-centered cubic structure. Mechanical testing revealed significant property improvements with the addition of AgNPs compared to the control. The 2 wt.% AgNP composite demonstrated optimal properties, including a 120% increase in tensile strength, a 246% increase in flexural strength, a 43.18% increase in hardness, and a 127% increase in impact resistance. The 2.5 wt.% AgNP composite exhibited optimum hardness and a 247% increase in flexural modulus, while the 3.0 wt.% AgNP composite had the highest tensile modulus with a 105% increase. These enhanced mechanical properties make the 2 wt.% AgNP composite suitable for demanding sustainable engineering applications, such as automotive systems, with the potential to reduce vehicle weight, improve fuel efficiency, and lower emissions. Additionally, it holds promise in renewable energy systems for cleaner energy generation and in water purification systems for use in filters or membranes, highlighting the potential of bio-synthesized AgNPs in advanced materials development.

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