Antibacterial and Antitumoral Activities of the Spider Acylpolyamine Mygalin Silver Nanoparticles

Courrol, Lilia Coronato; Espinoza-Culupú, Abraham; Silva, Pedro Ismael da; Gonçalves, Karina de Oliveira; Silva, Flávia Rodrigues de Oliveira; Borges, Monamaris Marques

doi:10.1007/s12668-020-00738-w

Cited by 5 publications

(5 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These last three biological activities have been demonstrated in in vitro and in vivo tests, with the antibacterial activity reported by Pereira [20] and Espinoza-Culupú, who showed the molecular mechanism of action in bacteria and their siderophoric activity [19]. In the case of anticancer activity, silver nanoparticles conjugated with mygalin [55] showed intense cytotoxic activity against cancer cell lines, thus confirming the in silico predictions.…”

Section: Discussionsupporting

confidence: 79%

See 1 more Smart Citation

Acylpolyamine Mygalin as a TLR4 Antagonist Based on Molecular Docking and In Vitro Analyses

et al. 2020

Self Cite

View full text Add to dashboard Cite

Toll-like receptors (TLRs) are transmembrane proteins that are key regulators of innate and adaptive immune responses, particularly TLR4, and they have been identified as potential drug targets for the treatment of disease. Several low-molecular-weight compounds are being considered as new drug targets for various applications, including as immune modulators. Mygalin, a 417 Da synthetic bis-acylpolyamine, is an analog of spermidine that has microbicidal activity. In this study, we investigated the effect of mygalin on the innate immune response based on a virtual screening (VS) and molecular docking analysis. Bone marrow-derived macrophages and the cell lines J774A.1 and RAW 264.7 stimulated with lipopolysaccharide (LPS) were used to confirm the data obtained in silico. Virtual screening and molecular docking suggested that mygalin binds to TLR4 via the protein myeloid differentiation factor 2 (MD-2) and LPS. Macrophages stimulated by mygalin plus LPS showed suppressed gene expression of tumor necrosis factor (TNF-α), interleukine 6 (IL-6), cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS), as well as inhibition of signaling protein p65 of the nuclear factor κB (NF-κB), resulting in decreased production of nitric oxide (NO) and TNF-α. These results indicate that mygalin has anti-inflammatory potential, being an attractive option to be explored. In addition, we reinforce the importance of virtual screening analysis to assist in the discovery of new drugs.

show abstract

Section: Discussionsupporting

confidence: 79%

“…Our data confirm that mygalin can interact with LPS (Figure 9). "iii 55,000 LPS-FITC-Myg 450 µM "' 30,000 "' 25,000…”

Section: Binding Of Mygalin To Lps-fitcmentioning

confidence: 99%

Acylpolyamine Mygalin as a TLR4 Antagonist Based on Molecular Docking and In Vitro Analyses

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…Ag NPs can act as anticancer agents [156,157]. The biocompatible Ag NPs are found to be effective against liver (Hep G2), lung (L-132), pancreas (MIA-Pa-Ca-2), breast (MDA-MB-231), oral (KB cells), prostate (PC-3), and cervical (HeLa) cancer cell lines, which confirms its potent anticancer action [54,158,159].…”

Section: Applications Of Silver Nanoparticlesmentioning

confidence: 71%

Current Research on Silver Nanoparticles: Synthesis, Characterization, and Applications

Dawadi

Katuwal

Gupta

et al. 2021

Journal of Nanomaterials

210

102

View full text Add to dashboard Cite

Over the past couple of decades, nanomaterials have advanced the research in materials; biomedical, biological, and chemical sciences; etc., owing to their peculiar properties at the nanoregime compared to their bulk composition. Applications of nanoparticles in the fields like medicine and agriculture have been boosted due to the development of different methodologies developed to synthesize specific shapes and sizes. Silver nanoparticles have tunable physical and chemical properties, so it has been studied widely to improve its applicability. The antimicrobial properties of Ag NPs are finding their application in enhancing the activity of drugs (like Amphotericin B, Nystatin, Fluconazole) and composite scaffolds for controlled release of drugs and targeted delivery of drugs due to their low toxicity and biocompatibility. Similarly, their surface plasmon resonance property makes Ag NPs a top-notch material for developing (bio)sensors, for instance, in surface-enhanced Raman spectroscopy, for detecting biomarkers, diseases, pollutants, and higher catalytic activity in photochemical reactions. Besides these, highly conducting Ag NPs are used in wearable and flexible sensors to generate electrocardiographs. Physicochemical or biological approaches are used to prepare Ag NPs; however, each method has its pros and cons. The prohibitive cost and use of hazardous chemicals hinder the application of physicochemical synthesis. Likewise, biological synthesis is not always reproducible for extensive use but can be a suitable candidate for therapeutic activities like cancer therapy. Excess use of Ag NPs is cytotoxic, and their unregulated discharge in the environment may have effects on both aquatic and terrestrial biota. The research in Ag NPs has always been driven by the need to develop a technology with potential benefits and minimal risk to environmental and human health. In this review, we have attempted to provide an insight into the application of Ag NPs in various sectors along with the recent synthetic and characterization techniques used for Ag NPs.

show abstract

“…Recently, the antimicrobial and antitumoral activities of seven mygalin silver nanoparticles (MygAgNPs) were evaluated by [29]. The MygAgNPs were synthetized by photoreduction method, forming spherical particles with sizes from 10 to 60 nm tested against E. coli, revealing a reasonable enhance in the antimicrobial activity (MICs between 19 and 58 nM according to mygalin concentration used to nanoparticle synthesis) when compared to the peptide native (MIC = 1 mM) form [29].…”

Section: Acanthoscurria Gomesianamentioning

confidence: 99%

“…The nanoparticle named as MygAgNP1 activity was evaluated against MCF-7 cells and normal NHI/3T3 murine fibroblast (ATCC ® CRL-1658) in doses from 2.5 to 80 µL of nanoparticles. Authors highlighted the 5 µL dose, which caused death of approximately 52% of the tumoral cells, but only 13% of the NHI/3T3 cells [29].…”

Section: Acanthoscurria Gomesianamentioning

confidence: 99%

Brazilian Theraphosidae: a toxicological point of view

Macedo

Costa

Souza

et al. 2021

J. Venom. Anim. Toxins incl. Trop. Dis

View full text Add to dashboard Cite

Antibacterial and Antitumoral Activities of the Spider Acylpolyamine Mygalin Silver Nanoparticles

Cited by 5 publications

References 29 publications

Acylpolyamine Mygalin as a TLR4 Antagonist Based on Molecular Docking and In Vitro Analyses

Acylpolyamine Mygalin as a TLR4 Antagonist Based on Molecular Docking and In Vitro Analyses

Current Research on Silver Nanoparticles: Synthesis, Characterization, and Applications

Brazilian Theraphosidae: a toxicological point of view

Contact Info

Product

Resources

About