Antibacterial and cytotoxic activity assessment of Channa striatus (Haruan) extract

Abstract

<jats:p>Background and Aim: Channa striatus extract, a freshwater snakehead fish known as Haruan, is popular in Southeast Asia for consumption and as a traditional therapeutic remedy for wound healing. C. striatus is also used in osteoarthritic for its anti-inflammatory. The aim of this study was to determine the presence of antibacterial properties of C. striatus extract against oral bacteria and to investigate the cytotoxic activity against Vero cells. Materials and Methods: The authors prepared C. striatus extract in chloroform-methanol solvents. Next, the authors took subgingival microbiological samples from 16 cats that had periodontal disease. The authors determined the antibacterial properties of C. striatus extract against the isolated bacteria using the disk diffusion method and a broth microdilution-based resazurin microtiter assay. Finally, the authors used the Vero cell line to evaluate the cytotoxic activity, and they assessed the cell availability using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Results: The results showed weak antibacterial activity of C. striatus extract against Pseudomonas spp. and Escherichia coli. In addition, the authors found that minimum inhibition concentration values ranged between 400 and 500 mg/mL, and minimum bactericidal concentration values ranged between 650 and 550 mg/mL. However, the cytotoxic results were promising, showing that C. striatus extract increased the cell viability and growth when it was at a higher concentration. The extract also promotes growth and cell proliferation. Conclusion: These findings suggest that C. striatus extract promoted cell proliferation in vitro and could be a plausible therapeutic wound healing alternative for periodontal disease in cats.</jats:p>

Antimicrobial activity, phytochemical characterization and gas chromatography-mass spectrometry analysis of Aspilia pluriseta Schweinf. extracts

“…To evaluate the general antimicrobial activity of A. pluriseta crude and solvent fractions extracts at various specified concentrations, we used the modified disc diffusion method [ 57 , 58 , 59 , 60 , 61 ]. A fresh microbial inoculum was made by suspending activated colonies in physiological saline.…”

“…The MIC and MMC of A. pluriseta solvent fractions were analyzed as previously described [ 36 , 57 , 60 , 61 , 63 ]. Briefly, 50 μL of varying fraction concentrations (3.9–500 μg/mL petroleum ether, dichloromethane, and methanol; 1.95–250 μg/mL ethyl acetate) were added into 100 μL of nutrient broth held in a sterile 96-well plate followed by addition of 50 μL test organisms adjusted to 0.5 McFarland standard.…”

In vitro cytotoxicity of Aspilia pluriseta Schweinf. extract fractions

“…The last column of microtiter well plate containing medium without plant solvent extract fractions, but with 1% DMSO, was included as the negative control. The percentage cell viability (%) was calculated at each concentration using the formula provided below [ 1 , 20 , 23 ]. …”

HaruanExtract (Channa striatus) as an Effective Mediator in Promoting Wound Healing

“…In recent years, extensive work has been conducted to analyse the antibacterial effects of the mucus of fish species [93][94][95][96][97], including the Channa species. Several research studies [48,[98][99][100][101][102][103][104] were performed over the years to evaluate the antibacterial and antifungal activities of Haruan. Most of the studies [99][100][101][102][103][104] revealed that Haruan displays some antimicrobial activities, except two studies [98,99] which detected negligible inhibitory effects against Staphylococcus aureus and Escherichia coli strains respectively.…”