Antibacterial and Cytotoxic Aspects of Longchain Fatty Acids as Cell Surface Events: Selected Topics

Fatty acid modifying enzyme (FAME) is an extracellular enzyme that inactivates staphylocidal lipids by catalysing the esterification of these lipids to cholesterol. In-vitro expression of FAME began at the start of the stationary phase. This expression of FAME was very similar to other staphylococcal extracellular proteins controlled by the global regulators Agr and Sar. A Stuphylococcus uureus strain ISP546 (Agr-) produced c. 80% less FAME than an isogenic Agr' strain ISP479C. Similar results were obtained with the isogenic Agr+/Agr-strain pair RN6390 and RN6911. A S. uureus strain R (Sar-) produced c. 86% less FAME than an isogenic Sar' strain RN6390. However, lipase assays on the same culture filtrates from the Sar+/Sar-strains did not demonstrate any affect on lipase production by the sur mutation.

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“…1). Similar results were obtained with a wild-type FAME-producing strain of S. aureus 182 [21] (data not shown).…”

Section: Genetic Regulation Of Fame Expressionsupporting

confidence: 87%

“…uureus strain 182 was a kind gift from Dr F. A. Kapral and has been characterised previously [21]. S. aureus strains ISP479C (Agr'), ISP546 (Agr-), RN6390 (Agr') and RN6911 (Agr-) were used to determine the role of the accessory gene regulator (Agr) in FAME production.…”

Section: Bacterial Strainsmentioning

confidence: 99%

Genetic regulation of fatty acid modifying enzyme from Staphylococcus aureus

Chamberlain

Imanoel²

1996

Journal of Medical Microbiology

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“…Palmitoleic acid-supplemented cells were the most virulent and these observations were statistically significant up to 0.1% level. Khuller et al (1982) and Mitchison et al (1961).…”

Section: Virulence Experimentsmentioning

confidence: 96%

“…All the animals, dead as well as sacrificed, were dissected and their tissues (lungs, spleens, livers) preserved for evaluation of the infection. On post-mortem examination, a score of 0 to 4 was given to the different organs as reported previously (Khuller et al, 1982), To examine the virulence, the total score for each animal was divided by its survival time in days to give an index, the 'root index' of virulence, described in detail by Mitchison et al, 1961, …”

Section: Virulence Experimentsmentioning

confidence: 99%

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LIPID COMPOSITION AND VIRULENCE OF MYCOBACTERIUM TUBERCULOSIS H₃₇Rv

Khuller

Taneja

Kaur

et al. 1982

Aust J Exp Biol Med

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Summary.The total lipids increased with decrease in total phospholipids in Mycobacterium tuberculosis H37RV grown at 30° as compared to 37°, Oleic and elaidic acid supplemented to the growth medium resulted in a higher total lipid and phospholipid content, but the content was lower in cells grown in the presence of palmitoleic acid. Cellular content of mannophosphoinositides decreased, whereas that of phosphatidylethanolamine and cardiolipin increased at the lower temperature, A decrease in mannophosphoinositides and an increase in cardiolipin were noted with all fatty acid supplements; however, phosphatidylethanolamine behaved differently. The unsaturated to saturated fatty acids ratio increased at low temperature with oleic and elaidic acid supplements, but it decreased with palmitoleic acid. Cells from media supplemented with palmitoleic acid were found to be most virulent to guinea-pigs. Possible correlation of altered lipid composition and virulence of M. tubercutosis H37RV has been discussed.

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