Antibacterial and cytotoxic properties of boron-containing dental composite

Abstract

Secondary dental caries are one of the major reasons for restoration replacements. Incorporating antimicrobial properties into dental materials would limit the initiation and progression of dental caries. In the current study, dental composites having 1%, 5%, and 10% (w/w) sodium pentaborate pentahydrate were prepared and analyzed for their mechanical properties, degree of monomer conversion (DC) rate, antibacterial effects against Streptococcus mutans, and biocompatibility with human dental pulp stem cells (hDPSCs). Incorporation of boron into the composites significantly decreased flexural strength and DC in a dose-dependent manner, but the value for 1% boron-containing composite still remained within acceptable levels. Compressive strength and diametral tensile strength were not found to be different from those of controls. Although no inhibition zone was detected in an agar-well diffusion assay for any materials tested, significant bacterial growth inhibition was obtained in a direct contact test for boron-containing composites. Immunocytochemical and lineage-specific gene expression analysis revealed that composites with boron content increased the osteogenic and odontogenic capacity of hDPSCs. Boron-containing dental composites showed promising results for future clinical applications, displaying nontoxic, osteogenic, and odontogenic-inducing characteristics with remarkable antibacterial activity against S. mutans, and are hence potentially able to prevent secondary caries.

Effects of short-time exposure of surface pre-reacted glass-ionomer eluate on dental microcosm biofilm

“…Although it is well-known that the ions in an S-PRG eluate are involved in various mechanisms, the antibiofilm mechanism has not yet been determined. According to previous studies, BO 3 3− is known to exhibit antibacterial activity and quorum sensing inhibition 20,21 . Dembitsky et al 22 demonstrated the inhibitory effect www.nature.com/scientificreports/ of BO 3 3on quorum sensing in fungi; this indicates the role of S-PRG in inhibiting quorum sensing, which is a key component in biofilm formation.…”

Development of a novel functionally graded membrane containing boron‐modified bioactive glass nanoparticles for guided bone regeneration

“…In previous studies, positive effect of B on osteogenic differentiation of MC3T3‐E1 pre‐osteoblastic cell was shown (Gümüşderelioğlu et al, ; Hakki, Bozkurt, & Hakki, ). Additionally, it has been reported that B addition enhanced the osteogenic and odontogenic differentiation potential of dental stem cells (Demirci et al, ; Taşlı, Doğan, Demirci, & Şahin, ). In our previous study, positive effect of B‐modified bioactive glass nanoparticles (BG‐NPs) on odontogenic differentiation of hDPSCs was observed (Moonesi Rad et al, ; Moonesi Rad Atila et al, ; Moonesi Rad et al, )…”

In vitro performance of a nanobiocomposite scaffold containing boron-modified bioactive glass nanoparticles for dentin regeneration

“…In conclusion, positive effect of MTA mixed with 5% CaCl 2 or 2.5% Na 2 HPO 4 was detected for mineralization of hDPSCs. 87 Demirci et al 15 prepared dental composites including 1%, 5%, and 10% (w/w) sodium pentaborate pentahdrate. Ten days after incubation of hDPSCs seeded composites in odontogenic medium, elevated levels of calcium deposition in all boron-containing composites were observed.…”

“…9 Different studies confirmed positive effects of B on proliferation of human osteoblasts and mesenchymal stem cells, 10,11 osteogenic differentiation of pre-osteoblastic cells, 12,13 and osteogenic and odontogenic differentiation of dental stem cells. 14,15 In our previous study, modification of BG with B promoted proliferation and odontoblastic differentiation of human dental pulp stem cells (hDPSCs). 16 Proteins, polysaccharides, and synthetic polymers constitute organic part of the scaffolds.…”