Antibacterial and fungicidal activities of ethanol extracts of 38 species of plants

Abstract

Galenic preparations are broadly used against microorganisms pathogenic to humans, thought their poteintial in this aspect is not studied completely. In our in vitro experiment we studied the influence of alcohol tinctures from 38 species of plants on 15 species of bacteria and one species of fungus. Zones of growth inhibition of colonies measuring over 8 mm were observed during the use of ethanol extracts of Maclura pomifera against eight species of microorganisms (Escherichia сoli, Proteus mirabilis, Serratia marcescens, Yersinia enterocolitica, Salmonella typhimurium, Rhodococcus equi, Campylobacter jejuni and Corynebacterium xerosis), Ginkgo biloba – against eight species (Enterococcus faecalis, S. marcescens, Y. enterocolitica, Klebsiella pneumoniae, Listeria іnnocua, L. monocytogenes, Р. аeruginosa and C. jejuni), Genista tinctoria – against seven species (E. coli, Enterobacter aerogenes, Proteus mirabilis, K. pneumoniae, S. typhimurium, Р. аeruginosa and Rh. equi), Phellodendron amurense – against seven species (E. faecalis, S. marcescens, S. typhimurium, Rh. equi, C. jejunі, C. xerosis and Candida albicans), Berberis vulgaris – against seven species (P. mirabilis, S. marcescens, K. pneumoniae, S. typhimurium, C. jejuni, Р. аeruginosa and C. xerosis), Vitex negundo – against six species (E. faecalis, E. coli, P. mirabilis, K. pneumoniae, S. typhimurium and Rh. equi), Koelreuteria paniculata – against six species (E. faecalis, P. mirabilis, S. marcescens, S. typhimurium, C. jejunі and E. coli), Magnolia kobus – against six species (E. faecalis, E. coli, P. mirabilis, S. marcescens, S. typhimurium, C. jejunі and C. xerosis), Liriodendron tulipifera – against six species (K. pneumoniae, Listeria іnnocua, Р. аeruginosa, C. jejuni, Rh. equi and C. albicans), Clematis flammula – against six species (E. faecalis, P. mirabilis, L. monocytogenes, Р. аeruginosa, C. jejuni and C. xerosis), Wisteria sinensis – against five species (E. coli, S. typhimurium, L. monocytogenes, Rh. equi and C. albicans), Chimonanthus praecox – against five species (E. faecalis, S. marcescens, L. monocytogenes, C. jejuni and Rh. equi), Colchicum autumnale – against five species (S. marcescens, K. pneumoniae, L. ivanovi, L. monocytogenes and Р. аeruginosa). As a result of the study, these plants were found to be the most promising for further study of in vivo antibacterial activity. In the search of antibacterial and antifungal activities, the following plants were observed to be less promising: Ailanthus altissima, Aristolochia manshuriensis, Artemisia absinthium, Callicarpa bodinieri, Campsis radicans, Catalpa duclouxii, Celastrus scandens, Dictamnus alba, Eucommia ulmoides, Geranium sanguineum, Laburnum anagyroides, Nepeta racemosa, Parthenocissus tricuspidata, Polygonatum multiflorum, Prunus dulcis, P. laurocerasus, Ptelea trifoliata, Pteridium aquilinum, Quercus castaneifolia, Q. petraea iberica, Salvia officinalis, Securigera varia, Styphnolobium japonicum, Tamarix elongata and Vitex agnus-castus.

Hematological parameters and protein metabolism in the blood of pregnant rats under the effect of vanadium citrate

“…Similar effects were observed for ethyl extract of the leaves of L. angustifolia: it notably inhibited growth of colonies of Salmonella typhimurium and Klebsiella pneumonia, weakly affected Escherichia coli, Proteus mirabilis and fungus of Candida albicans and inhibited no growth of cells of bacteria of Listeria monocytogenes and Corinebacterium xerosis (Zazharskyi et al, 2019). We seen broader range of antibacterial activity in in vitro experiments exhibited by ethyl extract from V. angus-castus that notably inhibited growth of Corinebacterium xerosis, Serratia marcescens, Salmonella typhimurium, Proteus mirabilis; weakly affected growth of colonies of Rhodococcus equi, Pseudomonas aeruginosa, Yersinia enterocolitica, Klebsiella pneumonia, Enterococcus faecalis, Escherichia coli, and took no inhibitory effect on growth of colonies of Enterobacter aerogenes, Listeria ivanovi, L. innocua, L. monocytogenes, Campylobacter jejuni and fungus of Candida albicans (Zazharskyi et al, 2020). Also, in our previous studies, we determined that aqueous tincture of V. angus-castus in in vitro experiment had weak lethal effect on larvae of parasitic intestinal nematodes of Strongyloides papillosus (Wedl, 1856), though mortality of nematodes of Haemonchus contortus (Rudolphi, 1803) in aqueous tincture of this plants was no different from the control (Boyko et al, 2020).…”

Ефективність Використання Про Біотичних Мікроорганізмів Bacillus Spp. Для Санітарних Обробок Поверхонь

“…Серія «Ветеринарна медицина», випуск 3 (54), 2021 of saline solutions reduces the spread of the pathogen L. monocytogenes (Shmychkova et al, 2021). The growth of pathogenic microorganisms is influenced by the antibacterial and fungicidal action of ethanol plant extracts and herbal infusions (Zazharskyi et al, 2019;Zazharskyi et al, 2020).…”

Bactericidal efficiency of preparation based on essential oils used in aerosol disinfection in the presence of poultry

“…The most significant parameter of disinfection efficiency is the reduction of microbial contamination of the air in poultry farms (Diadychkyna, 2011;Buckmaster, 2012;Addie et al, 2015). Disinfectants used in premises have a different focus and mechanism of action on microbial cells (Jiang et al, 2018;Maertens et al, 2018;Paliy et al, 2018;Zazharskyi et al, 2020). Currently, preparations of ammonium compounds and colloidal silver are widely used for disinfection (Park et al, 2009;Ding et al, 2019;Diaz et al, 2019).…”