Antibacterial and immunoregulatory activity of an antimicrobial peptide hepcidin in loach (Misgurnus anguillicaudatus)

Jin, Zeyu; Shen, Manli; Wang, Liuen; Wang, Cui; Gao, Miaomiao; Yu, Guoying; Chang, Zhongjie; Zhang, Xiaowen

doi:10.1016/j.ijbiomac.2023.124833

Cited by 7 publications

(3 citation statements)

References 74 publications

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“…Nevertheless, as occurred in our study with gilthead seabream cells, Nkl peptides failed to induce the IFN pathway in other fish species [15,34], even when they showed protective capacity upon viral infection [12,66,67]. In contrast, our study showed that the IFN pathway was likewise unaltered by the administration of Hamp and Dic peptides to European sea bass HK cells, as also occurred when administering Hamp and Dic encoding plasmids in European sea bass [11], Hamp plasmids in loach, or Hamp peptide in medaka (Oryzas latipes) [29,63]. Similar to what happened with Nkl, Hamp and Dic greatly down-regulated the IFN markers in gilthead seabream cells, revealing a completely different pattern of expression in both species.…”

Section: Discussioncontrasting

confidence: 43%

“…Otherwise, the potential relationship between cell-mediated cytotoxicity (CMC) and AMPs has been proposed in mammals [25], but not in teleosts, even considering the evidence that related Nkl and fish CMC [26][27][28]. Finally, cross-regulation amongst AMPs has been described in European sea bass [11] and loach (Misgurrus anguillicadatus) [8,29]. Taking all this into account, AMPs are shown to play an important role in fish immunity responses though further and deeper characterization of their functions is required.…”

Section: Introductionmentioning

confidence: 99%

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Synthetic Antimicrobial Peptides Fail to Induce Leucocyte Innate Immune Functions but Elicit Opposing Transcriptomic Profiles in European Sea Bass and Gilthead Seabream

Cervera,

Chaves-Pozo,

Cuesta

2024

Marine Drugs

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Antimicrobial peptides (AMPs) are promising molecules in diverse fields, including aquaculture. AMPs possess lytic effects on a wide range of pathogens, resulting in a potential replacement for traditional antimicrobials in aquaculture. In addition, they also have modulatory effects on host immune responses. Thus, the objective of this work was to evaluate the immunomodulatory capability of three known synthetic AMPs derived from European sea bass, NK-lysin (Nkl), hepcidin (Hamp), and dicentracin (Dic), in head-kidney cell suspensions from European sea bass and gilthead seabream. The tested peptides were neither cytotoxic for European sea bass nor gilthead seabream cells and failed to modulate the respiratory burst and phagocytosis activities. However, they modified the pattern of transcription of immune-related genes differently in both species. Peptides were able to promote the expression of marker genes for anti-inflammatory (il10), antiviral (mx, irf3), cell-mediated cytotoxicity (nccrp1, gzmb), and antibody responses (ighm) in European sea bass, with the Nkl peptide being the most effective. Contrary to this, the effects of those peptides on gilthead seabream mainly resulted in the suppression of immune responses. To conclude, European sea bass-derived peptides can be postulated as potential tools for immunostimulation in European sea bass fish farms, but more efforts are required for their universal use in other species.

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Section: Discussioncontrasting

confidence: 43%

Section: Introductionmentioning

confidence: 99%

Synthetic Antimicrobial Peptides Fail to Induce Leucocyte Innate Immune Functions but Elicit Opposing Transcriptomic Profiles in European Sea Bass and Gilthead Seabream

Cervera,

Chaves-Pozo,

Cuesta

2024

Marine Drugs

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“…17,18 Antimicrobial peptides (AMPs), a class of short and positively charged peptides, 19 have been extensively explored as potential alternatives to traditional antibiotics, due to their broad-spectrum antibacterial activity, 20 high specificity, 21 and capacity to modulate host immunity. 22 Typically, amphiphilic AMPs facilitate the formation of transmembrane ion channels on the bacterial cell membranes, disrupting the integrity of the membrane, 12 inducing cytoplasmic leakage, and leading to cell death. 23 Such a unique and physical action is less likely to promote bacterial resistance.…”

Section: ■ Introductionmentioning

confidence: 99%

Double-Network Hydrogel with Strengthened Mechanical Property for Controllable Release of Antibacterial Peptide

Tan,

Hou,

Zhang

et al. 2024

Biomacromolecules

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Developing double-network (DN) hydrogels with high mechanical properties and antibacterial efficacy to combat multidrug-resistant bacterial infections and serve as scaffolds for cell culture still remains an ongoing challenge. In this study, an ionresponsive antibacterial peptide (AMP) (C 16 -WIIIKKK, termed as IK7) was synergistically combined with a photoresponsive gelatin methacryloyl (GelMA) polymer to fabricate a biocompatible DN hydrogel. The GelMA-IK7 DN hydrogel showed enhanced mechanical properties in contrast to the individual IK7 and GelMA hydrogels and demonstrated substantial antibacterial efficacy. Further investigations revealed that the DN hydrogel effectively inhibited bacterial growth by the controlled and sustained release of the IK7 peptide. In addition, the formation of the DN hydrogel was also found to protect AMP IK7 from rapid degradation by proteinase K. Our findings suggested that the developed GelMA-IK7 DN hydrogel holds great potential for next-generation antibacterial hydrogels for three-dimensional cell culture and tissue regeneration.

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Advancements of fish-derived peptides for mucormycosis: a novel strategy to treat diabetic compilation

Velumani,

Arasu,

Issac

et al. 2023

Mol Biol Rep

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Antibacterial and immunoregulatory activity of an antimicrobial peptide hepcidin in loach (Misgurnus anguillicaudatus)

Cited by 7 publications

References 74 publications

Synthetic Antimicrobial Peptides Fail to Induce Leucocyte Innate Immune Functions but Elicit Opposing Transcriptomic Profiles in European Sea Bass and Gilthead Seabream

Synthetic Antimicrobial Peptides Fail to Induce Leucocyte Innate Immune Functions but Elicit Opposing Transcriptomic Profiles in European Sea Bass and Gilthead Seabream

Double-Network Hydrogel with Strengthened Mechanical Property for Controllable Release of Antibacterial Peptide

Advancements of fish-derived peptides for mucormycosis: a novel strategy to treat diabetic compilation

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