Antibacterial and modifying-antibiotic activities of the essential oils of Ocimum gratissimum L. and Plectranthus amboinicus L.

Aguiar, José Júnior; Sousa, Cicera P.B.; Araruna, Mariana K.A.; Silva, Maria K.N.; Portelo, Aline C.; Lopes, Jeferson C.; Carvalho, Victoria R.A.; Figueredo, Fernando Gomes; Bitu, Vanessa C.N.; Coutinho, Henrique Douglas Melo; Miranda, Thiago Adolfo Sobreira; Matias, Edinardo Fágner Ferreira

doi:10.1016/j.eujim.2014.10.005

Cited by 47 publications

(35 citation statements)

References 20 publications

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“…A caracterização química do OEOg identificou oito componentes, sendo o eugenol (51,84%) e o 1,8-cineol (23,81%) os constituintes majoritários, resultados que estão de acordo com valores descritos na literatura (Sousa et al, 2004, Aguiar et al 2015.…”

Section: Discussionunclassified

Atividade antifúngica e possível mecanismo de ação do óleo essencial de folhas de Ocimum gratissimum (Linn.) sobre espécies de Candida

Oliveira

Batista

Fernandes

et al. 2016

Rev. bras. plantas med.

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RESUMO:A espécie Ocimum gratissimum (Linn.), popularmente conhecida como alfavacacravo, é muito utilizada na medicina popular. A planta apresenta inúmeros compostos, sendo o eugenol o constituinte majoritário do seu óleo essencial e o provável responsável pela sua atividade antimicrobiana. O objetivo deste estudo foi determinar o potencial antifúngico e o possível mecanismo de ação do óleo essencial extraído das folhas de O. gratissimum L. (OEOg) sobre cepas-padrão de Candida. Para avaliação da atividade antimicrobiana foi determinada a Concentração Inibitória Mínima (CIM), o efeito do tempo de exposição, o efeito modulador na atividade de antifúngicos (ATF) de uso clínico e a ação do OEOg nas fases de crescimento exponencial e estacionário de leveduras do gênero Candida. O mecanismo de ação do OEOg foi verificado por captação do cristal violeta e avaliação da morfologia microbiana pela técnica de microcultivo. Também foi avaliada a toxicidade do OEOg sobre hemácias humanas. O OEOg apresentou boa atividade antifúngica sobre cepas de Candida, sendo capaz de reduzir a taxa de crescimento das cepas de Candida a partir de quatro horas de exposição, além de ter modulado positivamente a atividade do cetoconazol para C. tropicalis ATCC 13803 e reduzir o número de células viáveis em todas as fases de crescimento microbiano. O OEOg foi capaz de promover o aumento discreto da captação do cristal violeta e provocou alterações na micromorfologia das células de Candida spp., sugerindo que seu alvo de ação seja o envoltório celular. Observou-se baixa toxicidade do OEOg sobre hemácias humanas. Os resultados encontrados mostraram que o OEOg possui boa atividade sobre o gênero Candida, com mecanismo de ação mediado possivelmente pela ocorrência de danos no envoltório celular, além de ter sido observada baixa toxicidade, indicando do OEOg é promissor no desenvolvimento e elaboração de um novo fármaco com potencial atividade para o tratamento de doenças fúngicas. Palavras-chave:Ocimum, óleos essenciais, atividade antimicrobiana, farmacologia. ABSTRACT: Antifungal activity and potential action mechanisms of essential oil ofOcimum gratissimum (Linn.) leaves against Candida species. Ocimum gratissimum (Linn.) is a medicinal plant popularly known as "wild basil" widely used in traditional medicine. The plant has numerous compounds, and eugenol is the major constituent of its essential oil and likely responsible for its antimicrobial activity. The aim of this study was to determine the antifungal activity and the potential mechanism of action of the essential oil extracted from the leaves of O. gratissimum L. (OEOg) against standard strains of Candida. The following experiments were performed: determination of Minimum Inhibitory Concentration (MIC), determination of the effect of exposure time to OEOg; evaluation of the modulating effect of OEOg in antifungal (ATF) activity for clinical use; determination of the effects of the OEOg on different growth phases of Candida spp; determination of crystal violet (CV) uptake and the microcultu...

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Section: Discussionunclassified

Atividade antifúngica e possível mecanismo de ação do óleo essencial de folhas de Ocimum gratissimum (Linn.) sobre espécies de Candida

Oliveira

Batista

Fernandes

et al. 2016

Rev. bras. plantas med.

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“…This increasing interest is due to the scarcity of therapeutic agents available to treat chronic diseases, increasing of bacterial resistance and the harmful side effects of chemical compounds [39,40]. Natural plant products containing polyphenolic compounds such as phenolic acids, tannins, anthocyanins, and flavonoids were demonstrated to have potential health benefits for the treatment of metabolic disorders such as diabetes mellitus, hypercholesterolemia, overweight, and obesity [41].…”

Section: Discussionmentioning

confidence: 99%

“…The working solutions of different concentrations (1,2,3,5,7,10,20,30,40,50,80, and 100 μg/ml) were prepared by serial dilution of the stock solution with methanol. DPPH was freshly prepared at a concentration of 0.002% w/v.…”

Section: Dpph Assaymentioning

confidence: 99%

Investigation of the antiobesity and antioxidant properties of wild Plumbago europaea and Plumbago auriculata from North Palestine

Jaradat

Zaid

Hussein

2016

Chem. Biol. Technol. Agric.

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Background: Historically, plants have proven their value as a source of phytochemicals with therapeutic potentials and recently they play an important role in the discovery of novel drugs. Objectives:The current study aimed to investigate new antiobesity drugs from Plumbago europaea and Plumbago auriculata through the inhibition of the adsorption of dietary lipids. In vitro porcine pancreatic lipase inhibitory tests were conducted with weight reduction property as well as this study aimed to explore the antioxidant potential and to estimate total phenolic, flavonoid, and tannin contents in both of the studied species.Methods: Antioxidant capacity was evaluated using ferric reducing antioxidant power and DPPH assays, and porcine pancreatic lipase inhibitory tests were conducted using the UV spectrophotometric method, while total flavonoid, phenol, and tannin contents were estimated using standard phytochemical analytical methods.Results: Antioxidant potential and total flavonoid, phenol, and tannin contents of P. europaea were significantly higher than those of P. auriculata, and both of the studied species have potential antiobesity activity in comparison with orlistat. Conclusion:Results of the present study show that both of the studied Plumbago species consist of bioactive compounds that can act as lipase inhibitors and therefore can be useful for the development of functional foods against obesity. It can also be used as a source of lead compounds for designing new antiobesity drugs. Further isolation, identification, and characterization of phyto-active compounds responsible for antiobesity action are required to evaluate the full therapeutic potentials of these plants.

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“…Plectranthus amboinicus is a widespread vegetable in Asian cuisine and a long-standing herb in folk medicine for curing of common diseases such as fever, heat cough, inflammation throat, hoarseness, insect bite, treatment of diabetic foot ulcers [8,9]. Studies showed that the P. amboinicus had antibacterial activity in high organisms [10][11][12], therefore essential oils from the plant and their preparations have been increasingly utilized in manufacture of functional foods, pharmaceuticals and cosmetics. P. amboinicus oil was commonly obtained from the leaves and stems by extraction.…”

Section: Introductionmentioning

confidence: 99%

Extraction Process of Essential Oil from Plectranthus amboinicus Using Microwave-Assisted Hydrodistillation and Evaluation of It’s Antibacterial Activity

et al. 2018

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Plectranthus amboinicus oil has many applications in pharmaceutical, medicine and cosmetic industries. Recently, new methods of extracting essential oil used have been increasingly developed to replace traditional methods. In this study, maximization of essential oils yield from P. amboinicus was studied by the combination of microwave assisted hydro-distillation (MAHD) and response surface methodology (RSM). We found that that the maximum essential oil yield was 0.1374 % with 94.38 % reliability and influencing factors such as microwave power for this process was 515 W, raw material to water ratio 1.64:1, extraction time at 100.8 min. ANOVA analysis for quadratic model also gives favourable outcome including the high determination coefficient (R2 = 0.94), significant F-value and p-value of coefficients. Under laboratory condition, the obtained yield (0.1374 %) approximated the yield predicted by the quadratic model, suggesting the reasonable soundness of the employed model and RSM when it comes to optimizing the parameters of extraction. In addition, considerable antibacterial activities of extracted essential oil against four bacteria cultures, in particular, S. aureus was recognized.

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Antibacterial and modifying-antibiotic activities of the essential oils of Ocimum gratissimum L. and Plectranthus amboinicus L.

Cited by 47 publications

References 20 publications

Atividade antifúngica e possível mecanismo de ação do óleo essencial de folhas de Ocimum gratissimum (Linn.) sobre espécies de Candida

Atividade antifúngica e possível mecanismo de ação do óleo essencial de folhas de Ocimum gratissimum (Linn.) sobre espécies de Candida

Investigation of the antiobesity and antioxidant properties of wild Plumbago europaea and Plumbago auriculata from North Palestine

Extraction Process of Essential Oil from Plectranthus amboinicus Using Microwave-Assisted Hydrodistillation and Evaluation of It’s Antibacterial Activity

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