Antibacterial and non-cytotoxic ultra-thin polyethylenimine film

Hernández‐Montelongo, Jacobo; Lucchesi, Eliane Gama; Nascimento, V. F.; França, Carla Giometti; González, Ismael; Macedo, W. A. A.; Machado, Daisy; Lancellotti, Marcelo; Moraes, Ângela Maria; Beppu, Marisa Masumi; Cotta, M. A.

doi:10.1016/j.msec.2016.10.064

Cited by 22 publications

(13 citation statements)

References 39 publications

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“…For PEI functionalized surfaces it is known that cytotoxicity can exist depending on the molecular weight and concentration (Brunot et al, 2007). However, in studies with surfaces derived from low molecular weight PEI, as used here (25 kDa), no cytotoxic effects were reported for fibroblasts up to 7 days (Hernandez-Montelongo et al, 2017). PEI modification could further improve proliferation and function of MG-63s (Liu et al, 2009), as indicated here by an increased Ca 2+ slope.…”

Section: Moderately Positive ζ-Potential (+1 To +10 Mv)mentioning

confidence: 59%

Enhancement of Intracellular Calcium Ion Mobilization by Moderately but Not Highly Positive Material Surface Charges

Gruening

Neuber

Nestler

et al. 2020

Front. Bioeng. Biotechnol.

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Electrostatic forces at the cell interface affect the nature of cell adhesion and function; but there is still limited knowledge about the impact of positive or negative surface charges on cell-material interactions in regenerative medicine. Titanium surfaces with a variety of zeta potentials between −90 mV and +50 mV were generated by functionalizing them with amino polymers, extracellular matrix proteins/peptide motifs and polyelectrolyte multilayers. A significant enhancement of intracellular calcium mobilization was achieved on surfaces with a moderately positive (+1 to +10 mV) compared with a negative zeta potential (−90 to −3 mV). Dramatic losses of cell activity (membrane integrity, viability, proliferation, calcium mobilization) were observed on surfaces with a highly positive zeta potential (+50 mV). This systematic study indicates that cells do not prefer positive charges in general, merely moderately positive ones. The cell behavior of MG-63s could be correlated with the materials' zeta potential; but not with water contact angle or surface free energy. Our findings present new insights and provide an essential knowledge for future applications in dental and orthopedic surgery.

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Section: Moderately Positive ζ-Potential (+1 To +10 Mv)mentioning

confidence: 59%

Enhancement of Intracellular Calcium Ion Mobilization by Moderately but Not Highly Positive Material Surface Charges

Gruening

Neuber

Nestler

et al. 2020

Front. Bioeng. Biotechnol.

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“…This could confirm that the positive amino acid residues of PEI/Tet-124 are effectively oriented to the top surface, interacting with the cell surface as well. However, cell viability first decreased at 24 h and then increased at 72 h for PEI/Tet-124, which can be considered a temporary inhibition of proliferation due to adaptation mechanisms of the cells to this surface [24]. For the case of PEI/Tet-124-Br, the sample presented the lower antibacterial effect but the higher cell viability, possibly because the positive amino acid residues are not completely oriented to the surface top, thus exhibiting diminished interaction with both S. epidermidis bacteria and fibroblast cells.…”

Section: Resultsmentioning

confidence: 99%

“…Moreover, their surfaces were more heterogeneous with some randomly distributed peaks, which could be associated with local accumulation of peptides. PEI is used to promote uniform depositions of subsequent molecules, but a single film obtained by the dip coating technique is not completely homogenous; its surface can present some irregularities at the nanoscale level [24]. As peptides tend to aggregate and organize into large fibrils [25], those irregularities could present a higher charge that favors the aggregation of Tet peptides.…”

Section: Resultsmentioning

confidence: 99%

Electrostatic immobilization of antimicrobial peptides on polyethylenimine and their antibacterial effect against Staphylococcus epidermidis

Hernández‐Montelongo

Ureña²,

Machado

et al. 2018

Colloids and Surfaces B: Biointerfaces

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Staphylococcus epidermidis is a gram-positive bacterium, and one of the most prevalent causes of nosocomial infections due to its strong ability to form biofilms on catheters and surgical implants. Here we explore the antimicrobial properties of Tet-124 peptides, which are part of the innate defense against different multicellular organisms in nature. Two different Tet-124 peptides were immobilized on a polyethylenimine (PEI) film to determine their impact on the antimicrobial properties: KLWWMIRRW (Tet-124), which contains only natural amino acids, and KLWWMIRRWG-(F-Br)-G (F-Br = 4-Bromophenylalanine), a modified Tet-124 sequence with the addition of an unnatural amino acid. The immobilization was obtained as a result of the electrostatic interaction between PEI amino groups and the C-terminal carboxylic groups of tryptophan and glycine amino acids of Tet-124 and Tet-124-Br peptides, respectively. The process was monitored and studied by water contact angle, Atomic Force Microscopy (AFM), X-ray Photoelectron Spectroscopy (XPS) and Quartz Crystal Microbalance with Dissipation (QCM-D) measurements. The antibacterial effect of our samples against S. epidermis was evaluated by the spread plate counting method, and cytotoxicity was tested using fibroblast cultures. Our results indicate the feasibility to immobilize electrostatically both Tet-124 peptides for biomedical applications.

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“…Previous studies showed that multilayer thin films of PEI/heparin on NiTi alloy demonstrate better biocompatibility compared with NiTi alloy itself [88,89]. In addition, PEI demonstrated some antibacterial properties against S. aureus and P. aeruginosa after 24 h and its non-cytotoxicity against fibroblasts after 7 days [90]. However, other studies have shown that PEI is cytotoxic; some authors say that this cytotoxicity is molecular weight-dependent, with lower molecular weight preparations demonstrating lower cytotoxicity [91,92].…”

Section: Synthetic Polymer Coatingsmentioning

confidence: 99%

Amine-Rich Coatings to Potentially Promote Cell Adhesion, Proliferation and Differentiation, and Reduce Microbial Colonization: Strategies for Generation and Characterization

Martocq

Douglas

2021

Coatings

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Biomaterial surface modification represents an important approach to obtain a better integration of the material in surrounding tissues. Different techniques are focused on improving cell support as well as avoiding efficiently the development of infections, such as by modifying the biomaterial surface with amine groups (–NH2). Previous studies showed that –NH2 groups could promote cell adhesion and proliferation. Moreover, these chemical functionalities may be used to facilitate the attachment of molecules such as proteins or to endow antimicrobial properties. This mini-review gives an overview of different techniques which have been used to obtain amine-rich coatings such as plasma methods and adsorption of biomolecules. In fact, different plasma treatment methods are commonly used with ammonia gas or by polymerization of precursors such as allylamine, as well as coatings of proteins (for example, collagen) or polymers containing –NH2 groups (for example, polyethyleneimine). Moreover, this mini-review will present the methods used to characterize such coatings and, in particular, quantify the –NH2 groups present on the surface by using dyes or chemical derivatization methods.

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Antibacterial and non-cytotoxic ultra-thin polyethylenimine film

Cited by 22 publications

References 39 publications

Enhancement of Intracellular Calcium Ion Mobilization by Moderately but Not Highly Positive Material Surface Charges

Enhancement of Intracellular Calcium Ion Mobilization by Moderately but Not Highly Positive Material Surface Charges

Electrostatic immobilization of antimicrobial peptides on polyethylenimine and their antibacterial effect against Staphylococcus epidermidis

Amine-Rich Coatings to Potentially Promote Cell Adhesion, Proliferation and Differentiation, and Reduce Microbial Colonization: Strategies for Generation and Characterization

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