Antibacterial and osteogenic properties of silver‐containing hydroxyapatite coatings produced using a sol gel process

Abstract

Since bacterial infection is a rising complication following the wide use of implant, there is considerable attention on the effect of implant surface properties on bacterial adhesion. In this study, the effect of silver (Ag) doped hydroxyapatite (HA) coatings on initial antibacterial adhesion and osteoblast cell proliferation and differentiation was investigated. Using a sol-gel process, HA coatings doped with 1 wt % AgNO(3) (AgHA1.0) and 1.5 wt % Ag (AgHA1.5) were prepared. Coated surfaces were characterized using X-ray diffraction (XRD) and contact angles measurements. The initial bacteria adhesion was evaluated using a RP12 strain of Staphylococcus epidermidis (ATCC 35984) and the Cowan I strain of Staphylococcus aureus, whereas osteoblast proliferation and differentiation were evaluated using human embryonic palatal mesenchyme cells (HEPM), an osteoblast precursor cell line. In this study, XRD analysis of all surfaces indicated peaks corresponding to HA. Contact angles for AgHA surfaces were observed to be significantly lower when compared to HA surfaces. In vitro initial bacterial adhesion study indicated a significantly reduced number of S. epidermidis and S. aureus on AgHA surfaces when compared to HA surface. The use of HEPM cells indicated no significant difference in double-stranded DNA (dsDNA) production between all surfaces. Additionally, no differences in alkaline phosphatase specific activity were observed between HA and AgHA1.0 surfaces. Overall, it was concluded that AgHA1.0 has the similar biological activity as HA, with respect to bone cell proliferation and differentiation. In addition, the AgHA1.0 was also concluded to have the ability to minimize the initial bacteria adhesion. (c) 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2007.

Antimicrobial dental implant functionalization strategies —A systematic review

“…Pathogens found at infected oral implant sites, including S. mutans 90) , S. aureus 93,94) , S. oralis 43) , P. gingivalis and A. actinomycetemcomitans 84) were killed or significantly reduced upon contact with silver-implanted surfaces. All tested silver surfaces, such as hydroxyapatite (HA)-silver-surfaces 42,44,[46][47][48][49][50][51]91,161) or plasma sprayed silver-implanted HA 45,95) shared good antimicrobial activity.…”

An Excursion into the Intriguing World of Polymeric Tl(I) and Ag(I) Cyanoximates

“…In the United States alone more than 4.4 million people have at least one internal fixation device and more than 1.3 million people have an artificial joint [55]. Bacterial infection induced by implant placement is a significant rising complication and is associated with considerable morbidity and costs [56]. Clinical practice has shown that systemic antibiotics are unable to provide effective treatment for implant-associated infections.…”

Advances in Segmental Endoprosthetic Reconstruction for Extremity Tumors: A Review of Contemporary Designs and Techniques

“…Also, surgical techniques and device technology changed rapidly, often within the time frame of clinical studies, making the results diffi cult to interpret. [11][12][13][14][15][16] In addition, studies combined data from oncology, trauma, and adult reconstruction and included data from multiple anatomic locations. 2,3,9 These issues added to the complexity and inaccuracy of early outcome data, thereby making interpretation diffi cult.…”

“…These compounds have demonstrated no reduction in the bone ingrowth potential of standard hydroxyapatite. 12 Furthermore, nanotechnology has facilitated the manufacturing of more osteoconductive surface preparations that encourage more robust osseous ingrowth after implantation. 75 Similar to the proposed modifi cations to tumor endoprostheses already mentioned, porous prostheses with the capability to leach high local concentrations of antibiotics are also in design phase.…”