Antibacterial, anti-inflammatory and probiotic potential of Enterococcus hirae isolated from the rumen of Bos primigenius

Abstract

In the present study bacterial strains were isolated from the rumen fluids of Bos primigenius and investigated their in vitro probiotic properties with potent antibacterial activity and anti-inflammatory effects. 9 g positive bacterial isolates were obtained and three isolates could able to tolerate gastric conditions, high bile salt concentrations and exhibited significant bactericidal effect against the enteric pathogens Vibrio cholera, Enterococcus faecalis, Enterobacter aerogens, Pseudomonas aeruginosa, Escherichia coli and Salmonella typhi. Moreover it showed above 70% cell surface hydrophobicity, significant low-invasion ability and potential adherence capacity in Caco-2 cells when compared with the control. The proinflammatory cytokines (TNF-α) was greatly reduced in rumen bacteria treatment and ARBS-1 modulate the immune response by activating the IL-4 secretion in parallel to TNF-α suppression. The 16s rRNA gene sequence of the active isolates were identified as Enterococcus hirae (ARBS-1), Pediococcus acidilactici (ARBS-4) and Bacillus licheniformis (ARBS-7). This study revealed the probiotic bactericidal properties of E. hirae obtained from the rumen of B. primigenius with potential antibacterial and anti-inflammatory effects. Future studies with the strains may yield some novel probiotic product for livestock's.

Functional and health promoting inherent attributes ofEnterococcus hiraeF2 as a novel probiotic isolated from the digestive tract of the freshwater fishCatla catla

“…Results of this study validated that E. hirae F2 was surviving bile salts from 0.2% to 1.0% concentration, which is 4–5 times higher than the bile salt concentration of human intestine. However, previous studies (Gupta & Tiwari, 2015; Selvaraj et al, 2014) on E. hirae also indicated similar kind of results of bile salt tolerance. Further confirmation related to bile salt tolerance of E. hirae F2 was confirmed by the presence of BSH enzyme.…”

Influence of the intestinal microbiota on disease susceptibility in kittens with experimentally-induced carriage of atypical enteropathogenic Escherichia coli

“…While the specific mechanism of this observation was not established by this study, others have shown that the lipoteichoic acid of E. hirae is able to protect against loss of epithelial tight junction function induced by TNF-α (Miyauchi et al, 2008). Additionally, E. hirae can limit the secretion of inflammatory mediators induced by LPS (Arokiyaraj et al, 2014; Miyauchi et al, 2008) and exerts bactericidal effects against numerous pathogens including E. coli and E. faecalis (Miyauchi et al, 2008).…”

Characterization of probiotic potential of Bacillus species isolated from a traditional brine pickle

“…Adenocarcinoma cell line HT-29 was obtained from NCCS (National Centre for Cell Sciences), Pune, India and maintained in Dulbecco's modified Eagle's medium (DMEM, Himedia), with 10% fetal bovine serum (Himedia), 1X antibiotic-antimycotic solution (Himedia). Adhesion studies on HT-29 cells were performed as described by Arokiyaraj et al (2014). After incubation, the bacterial suspensions were removed carefully; stained with Giemsa stain for 20 min at room temperature followed by methanol fixation, and visualized under inverted microscope.…”