Antibacterial, Antibiofilm, Antiswarming, and Antioxidant Activities of Flavonoids Isolated from Allium colchicifolium Leaves

Majnooni, Mohammad Bagher; Ghanadian, Syed Mustafa; Mojarrab, Mahdi; Bahrami, Gholamreza; Mansouri, Kamran; Mirzaei, Arezoo; Farzaei, Mohammad Hossain

doi:10.1155/2023/5521661

Cited by 4 publications

(1 citation statement)

References 71 publications

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“…Mekanisme ketiga dengan menghambat fungsi membran sel dengan membentuk senyawa protein ekstraseluler sehingga menimbulkan kerusakan pada membran sel. 23,28 Penelitian Majnooni et al, 29 menunjukkan hasil sesuai pernyataan di atas bahwa flavonoid memiliki aktivitas antibakteri dan antibiofilm terhadap S. aureus dan Proteus mirabilis.…”

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Daya antibakteri pasta gigi yang mengandung ekstrak biji kopi robusta (Coffea canephora) terhadap Treponema denticola: eksperimental laboratoris

Perdana,

Praharani,

Sari

2024

Padjadjaran J Dent Res Students

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Section: Pembahasanunclassified

Daya antibakteri pasta gigi yang mengandung ekstrak biji kopi robusta (Coffea canephora) terhadap Treponema denticola: eksperimental laboratoris

Perdana,

Praharani,

Sari

2024

Padjadjaran J Dent Res Students

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Flavonoids Isolated From Delphinium semibarbatum Flowering Aerial Parts With Their Antibacterial, Antibiofilm, and Antiswarming Activity Against Proteus mirabilis and Staphylococcus aureus

Lotfaliani,

Ayatollahi,

Kobarfard

et al. 2024

Journal of Food Biochemistry

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Proteus mirabilis and Staphylococcus aureus are pathogens associated with CAUTIs. Understanding their significance is crucial for effective prevention and treatment. In this study, the Delphinium semibarbatum flavonoid‐rich fraction (DSF) was analyzed, and its antibacterial, antibiofilm, and antiswarming properties against these two bacterial species were evaluated. In the phytochemical analysis, four flavonoids, including kaempferol, 4′‐O‐methyl quercetin, quercetin, and kaempferol‐3‐O‐beta‐D‐glucopyranoside (K3G), were isolated. The minimum inhibitory concentration (MIC50) of DSF on P. mirabilis and S. aureus was 1000 μg/mL. Similarly, kaempferol, K3G, and 4′‐O‐methyl quercetin exhibited inhibitory effects of 20%, 13%, and 12%, respectively, at a concentration of 1000 μg/mL compared to the P. mirabilis control. However, the MIC50 of kaempferol and 4′‐O‐methyl quercetin for S. aureus were 125 μg/mL and 62.25 μg/mL, respectively. K3G exhibited an inhibitory effect of 37% at a concentration of 1000 μg/mL. DSF reduced biofilm at 1 mg/mL by 78% and 74% for P. mirabilis and S. aureus, respectively. Quercetin was the most effective in inhibiting P. mirabilis biofilm formation, with a 96% inhibition rate, followed by kaempferol and K3G, with 67% and 29%, respectively, at the same concentration. The best bioactive compound against biofilm inhibition of S. aureus was quercetin at 250 μg/mL, exhibiting 90% inhibition, followed by K3G, 4′‐O‐methyl quercetin, and kaempferol with 81%, 45%, and 33%, respectively, at a concentration of 125 μg/mL. In the swarming inhibition assay, DSF showed 78% inhibition at concentrations of 5 μg/mL. Similarly, all bioactive compounds showed 45% antiswarming activity at this concentration. The docking results of the isolated flavonoids on two target proteins, SarA and MrpH, showed that the presence of the methoxy group in the structure of 4′‐O‐methyl quercetin reduced its interaction with these two proteins. In the ADMET prediction, isolated flavonoids showed good predicted properties suitable for treating these two microbial species in urinary infection diseases.

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The effect of soaking heat-polymerized acrylic resin denture base in avocado seed extract (Persea americana Mill.) on the inhibition of denture-plaque microorganisms biofilm growth

Angela,

Wahyuni,

Halim

2024

F1000Res

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Background Heat polymerized acrylic (HPA) resins are known to have high porosity that contributes to increased surface roughness and microcrack formation in stress areas. This facilitates the attachment and growth of polymicrobial biofilms contributing to increased antimicrobial resistance. Many research had been carried out on avocado seeds, but no research that studies the effect of avocado seeds on denture-plaque microorganism biofilm on HPA resin has been found. Methods This study used 144 samples (n=144), namely HPA resin discs covered with mono-species and polymicrobial biofilms consisting of Candida albicans, Candida glabrata, Actinomyces odontolyticus, Streptococcus gordonii, and Staphylococcus aureus. The discs were soaked for 8 hours in the 5%, 10%, 15%, 20% avocado seed extract, positive control (alkaline peroxide), and negative control (aquadest). Each disc was shaken with a vortex mixer for 1 minute, and 100 μL was added into 96-well microplates with three times repetition and incubated for 24 hours. The inhibition values were determined from the percentage inhibition value formula which required absorption values from a microplate reader (595 nm). Results In this research, it was found that the MBIC50 of avocado seed extract against the mono-species of C. albicans (5%), C. glabrata (5%), A. odontolyticus (15%), S. gordonii (15%), S. aureus (10%), while against the biofilm was 20%. There was a significant effect of soaking HPA resin in avocado seed extract of 5%, 10%, 15%, 20% on the inhibition of mono-species and polymicrobial biofilms of denture-plaque microorganisms with a value of p<0.001 (p<0.05). Conclusion The MBIC50 of avocado seed extract in polymicrobial biofilm group was higher than that in the mono-species biofilm groups. Although alkaline peroxide showed higher inhibition value than that of the MBIC50 in polymicrobial biofilm group, 20% avocado seed extract was effective in inhibiting polymicrobial biofilm because it was able to inhibit more than 50% polymicrobial biofilm.

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Antibacterial, Antibiofilm, Antiswarming, and Antioxidant Activities of Flavonoids Isolated from Allium colchicifolium Leaves

Cited by 4 publications

References 71 publications

Daya antibakteri pasta gigi yang mengandung ekstrak biji kopi robusta (Coffea canephora) terhadap Treponema denticola: eksperimental laboratoris

Daya antibakteri pasta gigi yang mengandung ekstrak biji kopi robusta (Coffea canephora) terhadap Treponema denticola: eksperimental laboratoris

Flavonoids Isolated From Delphinium semibarbatum Flowering Aerial Parts With Their Antibacterial, Antibiofilm, and Antiswarming Activity Against Proteus mirabilis and Staphylococcus aureus

The effect of soaking heat-polymerized acrylic resin denture base in avocado seed extract (Persea americana Mill.) on the inhibition of denture-plaque microorganisms biofilm growth

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