Antibacterial, antifungal and wound healing potentials of extract and fractions of Nauclea diderrichii root bark

Abstract

Research Article IJTCM (2017), 2:1 Antibacterial, antifungal and wound healing potentials of extract and fractions of Nauclea diderrichii root bark This study was aimed to evaluate antimicrobial and wound healing effects of root bark extract and fractions of Nauclea diderrichii Merrill (Rubiaceae). Dried root barks of N. diderrichii was extracted using methanol and dichloromethane (1:1) to obtain methanol-dichloromethane extract (MDE). The MDE was fractionated using chromatographic techniques to obtain n-hexane (HF), ethylacetate (EF) and methanol (MF) fractions. The antimicrobial effect was tested against selected organisms using agar diffusion and agar dilution technique to determine the inhibition zone diameters (IZD) and minimum inhibitory concentrations (MICs). The excision wound model was used to assess the wound healing properties of MDE (5, 10 and 20%) and compared it with that of bacitracin and neomycin powder (BNP, standard) in rats. Phytochemical screening and acute toxicity test were performed using standard procedures. Results showed that extract and fractions exhibited antimicrobial effect with IZD (mm, 40 mg/ml) against tested organisms. The EF exhibited the