Antibacterial, Antifungal, Insecticidal and Phytotoxic activities of Abies pindrow leaves

Abstract

The current study was carried out to investigate the bioactivities of various extract fractions of Abies pindrow leaves. All fractions exhibited no anti-bacterial activity against all the tested bacterial strains. In case of antifungal study, n-hexane, ethyl acetate, chloroform, and residue fractions showed 20, 30 and 10% minimum inhibition at concentration of 20μg/mL, against Microsporum canis and Fusarium solani. The data obtained from insecticidal assay revealed that all the tested fractions shows no activity against the insects Tribolium castaneum, Rhyzopertha dominica and Callos bruchuanalis, except n-hexane extract fraction which display 20% bioactivity against Callos bruchuanalis. Chloroform fraction shows 20% activity against Tribolium castaneum, Rhyzopertha dominica and 40% against Callos bruchuanalis respectively. The water fraction which revealed 40% activity against Rhyzopertha dominica and 20% against Callos bruchuanalis respectively. The residue fraction shows 20% activity against Rhyzopertha dominica. Results of phytotoxicity revealed that the n-hexane, ethyl acetate, chloroform, water and residue fractions showed good phytotoxic activity at 1000μg/ml concentration. All the fractions showed moderate activity at 100 μg/ml concentrations except the n-hexane and residue fractions which showed 60 and 35% activity at 100 μg/ml concentrations respectively. At concentration of 10 μg/ml all the fractions showed low activity except chloroform fraction which shows no activity at 10μg/ml at all.