Antibacterial, antioxidant, and topical anti-inflammatory activities ofBergia ammannioides: A wound-healing plant

Abstract

Context: Despite the traditional use of Bergia ammannioides Henye ex Roth. (Elatinaceae) for the treatment of wounds in India, there is a scarcity of scientific data supporting this use. Objective: The objective of this study is to assess wound-healing potentiality of the plant, to study pharmacological activities that may contribute in eliminating wound complications, and to investigate the biologically active fractions. Material and methods: The ethanolic extract (EtOH) of the aerial parts was fractionated to obtain n-hexane (HxFr), chloroform (ClFr), ethyl acetate (EtFr), and n-butanol (BuOH) fractions. EtOH and its fractions were formulated in strength of 5 and 10% w/w ointment and tested for wound-healing activity using the excision model. The topical anti-inflammatory, in vitro antioxidant, and antibacterial activities were evaluated. HxFr and EtFr were chemically investigated to isolate their constituents. Results: Application of EtOH, HxFr, and EtFr (10% w/w ointments) leads to 71.77, 85.62, and 81.29% healing of the wounds with an increase in the collagen content. HxFr had the strongest anti-inflammatory (64.5% potency relative to Voltaren Õ ) and antibacterial activity (MIC ¼ 104 mg/ml against Staphylococcus aureus), while EtFr showed the strongest antioxidant activity against DPPH, ABTS + , and super oxide radical with an IC 50 value of 10.25 ± 0.01, 66.09 ± 0.76, and 167.33 ± 0.91 mg/ml, respectively. b-Sitosterol, lupeol, cyclolaudenol, and cycloartenol were isolated from HxFr. Quercetin, ellagic acid, kaempferol-3-O-a-L-rhamnoside, and quercetin-3-O-a-L-rhamnoside were isolated from EtFr. Discussion and conclusion: Our study presents scientific evidence for the efficacy of B. ammannioides in enhancing wound healing, and the first isolation of cyclolaudenol and cycloartenol from Bergia.

Gastro-Protective and Anti-Oxidant Potential of Althaea officinalis and Solanum nigrum on Pyloric Ligation/Indomethacin-Induced Ulceration in Rats

“…Free radical scavenging drugs, either from synthetic or herbal sources, have an important role in decreasing, de-activating, and eliminating reactive oxygen species (ROS). Accordingly, these drugs exert a significant protection for tissues from oxidative damage as well as enhancement of wound healing [63]. Many previous investigations documented the major role of oxidative stress in the pathogenesis of many diseases, including gastric ulcers [64].…”

Nano formulated proanthocyanidins as an effective wound healing component

“…A delay in the development of these mechanisms leads to failure or prolong wound healing process [3][4][5][6][7][8]. There are various topical and systemic agents used for the cure of wound and antimicrobial agents which are effective in preventing infections in surgical and traumatic wounds [9][10][11][12]. However, these topical agents delays re-epithelialisation and might pose a risk of oversensitivity and resistance [13][14][15].…”

Antioxidant, Cytotoxic and Antibacterial Activity and Total Phenol Contents of the Roots and the Shoots of Euphorbia macrostegia and Euphorbia microsciadia

“…Co-TLC analyses of the extracts along with common phytochemicals 1, 2, 3 and 4 were performed using silica gel (Figure 6, (1)) and AgNO3 -silica gel stationary phases (Figure 6, (2), ( 3)). In literature, compound 1 and 4 showed antioxidant and cytotoxic activity, while compound 3 showed antibacterial activity (20)(21)(22)(23)(24). Compounds 1 and 2 are present in almost all extracts in different ratios (Figure 6 (1), ( 3)), while the presence of compound 3 was confirmed in the MeOH extracts of the shoots of E. microsciadia, the roots of E. macrostegia, 80% MeOH extracts of the roots of E. microsciadia and the shoots of E. macrostegia and all the DCM extracts.…”