Antibacterial Collagen‐Based Nanocomposite Dressings for Promoting Infected Wound Healing

Abstract

Pathogenic bacterial infection is the most frequent wound complication, which has become a major clinical and healthcare challenge in wound management worldwide, leading to impaired healing processes, the risk of amputation, and even death. Here, collagen-based nanocomposite dressings (APZC) with broad-spectrum antibacterial activity are developed to promote the infected full-thickness wound healing. Short rod-like shaped ZnO NPs are synthesized and then coated with polydopamine (PDA) to obtain PDA coated ZnO NPs (PDA@ZnO NPs). Afterward, PDA@ZnO NPs are conjugated on the backbone of a collagen chain, and the obtained collagen-PDA@ZnO NPs conjugate is crosslinked by dialdehyde sodium alginate to fabricate APZC dressings. PDA@ZnO NPs show well dispersibility and are uniformly incorporated into the collagen matrix. APZC dressings have interconnected microporous structure and great physicochemical properties, besides good blood coagulation performance and well cytocompatibility. APZC dressings demonstrate long-lasting and excellently broad-spectrum antimicrobial activity, which can relieve the inflammatory reaction by killing pathogenic bacteria and induce the generation of blood vessels and the orderly deposition of collagen in the wound site, thus promoting infected full-thickness wound healing without obvious scar formation. Overall, the functionalized collagen-based nanocomposite dressings have great potential in the clinical treatment against bacteria-associated wound infection.

Dissolving Hyaluronic Acid-Based Microneedles to Transdermally Deliver Eugenol Combined with Photothermal Therapy for Acne Vulgaris Treatment

“…The cytotoxicity of microneedles was measured by the slightly modified CCK-8 assay . A high-glucose medium, 100 IU/mL penicillin–streptomycin solution, and fetal bovine serum were mixed at a volume ratio of 100:10:1 to prepare a complete culture medium.…”

“…The cytotoxicity of microneedles was measured by the slightly modified CCK-8 assay. 38 A high-glucose medium, 100 IU/mL penicillin−streptomycin solution, and fetal bovine serum were mixed at a volume ratio of 100:10:1 to prepare a complete culture medium. Microneedles were sterilized under UV irradiation before adding into a complete medium to obtain the sample culture medium.…”

Antioxidant, hemostatic, and injectable hydrogels with photothermal antibacterial activity to accelerate full-thickness wound regeneration

“…27,28 However, the majority of the existing PDA-based hydrogels require complex modification or synthesis, which might hinder them from further application. 29–31 Therefore, it is imperative to develop PDA-based injectable hydrogels through facile preparation. 32…”

Hydrophobic Tetracycline Immobilized in Fibrous Hyaluronan Regulates Adhesive Collagen‐Based Hydrogel Stability for Infected Wound Healing

“…[ 8 ] Dopamine‐coated zinc oxide nanofibers have also been introduced into the collagen matrix and cross‐linked with sodium dialdehyde alginate to stabilize the collagen structure and achieve durable and excellent broad‐spectrum antibacterial activity. [ 9 ] As the main components of the small intestinal submucosa (SIS) membrane are types I and III collagen, collagen‐binding peptides have been used to construct chimeric peptides with antibacterial osteogenic ability in SIS, with the aim of providing some antimicrobial activity in bone regeneration. [ 10 ] However, the long‐term safety of metal ions and the high cost of using antimicrobial peptides limit their clinical use.…”