Antibacterial effect against foodborne bacteria of plants used in traditional medicine in central Mexico: Studies in vitro and in raw beef

Presence of antibiotic‐resistant Salmonella strains on raw carrots was evaluated, as was the antibacterial activity of roselle calyx extracts against any identified resistant Salmonella strains. One hundred samples of carrots were collected from markets in Pachuca, Mexico. Salmonella was isolated from the carrot samples by culture procedure. Susceptibilities to sixteen antibiotics were determined for the isolated Salmonella strains by standard test. Antibacterial activity versus the isolated strains was measured for roselle extracts produced with water, methanol, acetone and ethyl acetate. Finally, the antibacterial effect of roselle extracts, sodium hypochlorite, acetic acid and colloidal silver against Salmonella strains isolated from carrots was evaluated on contaminated carrots. Thirteen Salmonella strains were isolated from 11% of the carrot samples. All strains exhibited resistance to at least two of the tested antibiotics. Roselle extracts caused a greater reduction in concentration of Salmonella than the sodium hypochlorite, acetic acid and colloidal silver on contaminated carrots. Practical Applications Roselle Hibiscus sabdariffa calyx extracts exhibited antimicrobial activity on culture media and on carrots against multidrug‐resistant Salmonella strains. The information presented shows that water extract of roselle calyx may be a potentially useful addition to disinfection procedures of raw carrots in field, carrot processor plants, restaurants and home.

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“…Extracts were obtained following a previously described method (Cruz‐Gálve et al . ; Morales‐Cabrera et al . ).…”

Section: Methodsmentioning

confidence: 98%

Antimicrobial Activity of Roselle Hibiscus Sabdariffa Calyx Extracts on Culture Media and Carrots Against Multidrug‐Resistant Salmonella Strains Isolated from Raw Carrots

Gutiérrez‐Alcántara

Gómez‐Aldapa

Román‐Gutiérrez

et al. 2016

Journal of Food Safety

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“…Roselle calyx extracts were produced following a previously described method (Cruz‐Gálvez et al . ; Morales‐Cabrera et al . ).…”

Section: Methodsmentioning

confidence: 98%

“…Roselle calyx extracts were produced following a previously described method (Cruz-G alvez et al 2013;Morales-Cabrera et al 2013). Briefly, dried calyces (100 g) were weighed, placed in sterile glass flasks and 900 ml methanol, acetone or ethyl acetate added to each flask.…”

Section: Extract Productionmentioning

confidence: 99%

Antibacterial effect of roselle extracts (Hibiscus sabadariffa ), sodium hypochlorite and acetic acid against multidrug-resistant Salmonella strains isolated from tomatoes

Gutiérrez‐Alcántara

Rangel‐Vargas

Gómez‐Aldapa

et al. 2016

Lett Appl Microbiol

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The presence of multidrug-resistant Salmonella in vegetables is a significant public health concern. Multidrug-resistant Salmonella strains were isolated from raw tomatoes purchased in public markets in Mexico and challenged with roselle Hibiscus sabdariffa calyx extracts, sodium hypochlorite and acetic acid. On tomatoes, the extracts caused a greater reduction in the concentration of antibiotic-resistant Salmonella strains than sodium hypochlorite and acetic acid. Roselle calyx extracts are a potentially useful addition to disinfection procedures of raw tomatoes in the field, processing plants, restaurants and homes.

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“…The filtered extracts were concentrated in a rotary evaporator (V-800, Vacuum Controller, BÜCHI, Switzerland). The acetone was completely removed from the rotaevaporated concentrate by placing it in an air recirculation oven (Ambi-Hi-Low Chamber, Lab-Line, Jefferson, MO, USA) at 45 • C for 24 h [22,31].…”

Section: Preparation Of Hibiscus Sabdariffa Extractmentioning

confidence: 99%

Hibiscus Acid and Chromatographic Fractions from Hibiscus Sabdariffa Calyces: Antimicrobial Activity against Multidrug-Resistant Pathogenic Bacteria

et al. 2019

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The anti-microbial properties of acetone extracts from Hibiscus sabdariffa calyces, fractions isolated by silica gel chromatography and hibiscus acid purified from some of these fractions and additionally identified by nuclear magnetic resonance spectroscopy, mid-infrared spectroscopy and X-ray diffraction, were studied against both multidrug-resistant Salmonella strains and pathogenic Escherichia coli bacteria. Gel diffusion was used to determine the anti-microbial effects. The mode of action of hibiscus acid was determined by crystal violet assay. Hibiscus acid and 17 of the 25 chromatographic fractions obtained, displayed an anti-microbial effect against all bacterial strains tested. Hibiscus acid showed a greater anti-microbial effect than the acetone extract against most of the bacteria strains, while chromatographic fractions IX–XIV exerted the greatest anti-microbial effect against all bacteria. The minimum inhibitory concentration of the acetone extract was 7 mg/mL, and the minimum bactericidal concentration was 10 mg/mL, while the corresponding values for hibiscus acid were 4–7 and 7 mg/mL, respectively. The results of the crystal violet assay indicate that hibiscus acid alters membrane permeability. Hibiscus acid is a potential alternative to control multidrug-resistant bacteria. Due to its ready availability and easy extraction from H. sabdariffa, hibiscus acid is potentially useful in the food industries.

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Antibacterial effect against foodborne bacteria of plants used in traditional medicine in central Mexico: Studies in vitro and in raw beef

Cited by 22 publications

References 28 publications

Antimicrobial Activity of Roselle Hibiscus Sabdariffa Calyx Extracts on Culture Media and Carrots Against Multidrug‐Resistant Salmonella Strains Isolated from Raw Carrots

Antimicrobial Activity of Roselle Hibiscus Sabdariffa Calyx Extracts on Culture Media and Carrots Against Multidrug‐Resistant Salmonella Strains Isolated from Raw Carrots

Antibacterial effect of roselle extracts (Hibiscus sabadariffa ), sodium hypochlorite and acetic acid against multidrug-resistant Salmonella strains isolated from tomatoes

Hibiscus Acid and Chromatographic Fractions from Hibiscus Sabdariffa Calyces: Antimicrobial Activity against Multidrug-Resistant Pathogenic Bacteria

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