Antibacterial effect of autologous platelet gel enriched with growth factors and other active substances

Abstract

Platelet-rich plasma is a new inductive therapy which is being increasingly used for the treatment of the complications of bone healing, such as infection and nonunion. The activator for platelet-rich plasma is a mixture of thrombin and calcium chloride which produces a platelet-rich gel. We analysed the antibacterial effect of platelet-rich gel in vitro by using the platelet-rich plasma samples of 20 volunteers. In vitro laboratory susceptibility to platelet-rich gel was determined by the Kirby-Bauer disc-diffusion method. Baseline antimicrobial activity was assessed by measuring the zones of inhibition on agar plates coated with selected bacterial strains. Zones of inhibition produced by platelet-rich gel ranged between 6 mm and 24 mm (mean 9.83 mm) in diameter. Platelet-rich gel inhibited the growth of Staphylococcus aureus and was also active against Escherichia coli. There was no activity against Klebsiella pneumoniae, Enterococcus faecalis, and Pseudomonas aeruginosa. Moreover, platelet-rich gel seemed to induce the in vitro growth of Ps. aeruginosa, suggesting that it may cause an exacerbation of infections with this organism. We believe that a combination of the inductive and antimicrobial properties of platelet-rich gel can improve the treatment of infected delayed healing and nonunion.

Intramammary administration of platelet concentrate as an unconventional therapy in bovine mastitis: First clinical application

“…Moreover, as no statistically significant differences in the average values of LS at T30 were observed between PC-only-or antibiotic-only-treated quarters, it is possible to speculate that PC alone is able, in addition to its regenerative properties, to exert also an antibacterial effect. Bielecki et al (2007) described that human PC in vitro inhibits the growth of Staphylococcus aureus and is also active against Escherichia coli. The α-granules of platelets do not only contain growth factors and antimicrobial peptides, but also catecholamines, serotonin, osteonectin, von Willebrand factor, and other substances that are released in high concentrations after platelet aggregation, and many of these factors have antibacterial action.…”

Buffered Platelet-Rich Plasma Enhances Mesenchymal Stem Cell Proliferation and Chondrogenic Differentiation

“…28 Interestingly, PRP has also been shown to have antimicrobial effects in vitro. 6 When combined with the potential for simple autologous sourcing, these properties give PRP great potential in regenerative medicine and as an adjuvant in tissue engineering.…”

Bio Technological Intervention with Platelet Rich Plasma for Assisted Regeneration of Sole

“…This is very significant as it indicates a very strong antimicrobial properties of PRP [31,32]. It has the potential to eliminate role of antibiotics in wound care, thus having a huge positive impact on antimicrobial resistance issues.…”

“…Previous studies of PRP have demonstrated antimicrobial activity against Escherichia coli, Staphylococcus aureus [31,32], including methicillin-resistant Staphylococcus aureus [31], Candida albicans [31] and Cryptococcus neoformans [33]. This suggests its role as antimicrobial and has the potential to eradicate the infective pathogens from the site and boost the healing process.…”

“…Platelets in PRP also play a role in host defence mechanism at the wound site by producing signalling proteins that attract macrophages [31]; PRP also may contain a small number of leukocytes [32] that synthesize interleukins as part of a non-specific immune response. Previous studies of PRP have demonstrated antimicrobial activity against Escherichia coli, Staphylococcus aureus [31,32], including methicillin-resistant Staphylococcus aureus [31], Candida albicans [31] and Cryptococcus neoformans [33].…”