Antibacterial effect of biosynthesized silver nanoparticles in Pacific white shrimp Litopenaeus vannamei (Boone) infected with necrotizing hepatopancreatitis bacterium (NHP B)

Abstract

ABSTRACT. The shrimp necrotizing hepatopancreatitis is caused by a pleomorphic Gram-negative Rickettsialike bacteria known as NHP-B. It is well known that silver is an effective bactericidal, but experiments with aquatic organisms are scarce, even more at nanoparticle level. The objective of this study was to determine the antibacterial effect of silver nanoparticles (AgNP) against NHP-B in the Pacific white shrimp Litopenaeus vannamei. For this, AgNO3 was used as source of silver, and extracts of dried leaves of green tea Camellia sinensis and neem tree Azadirachta indica as reducing agents. Different lots of infected shrimp received 0,5 and 35 μg of AgNP by forced feeding. The differences between the number of bacterial nodules in shrimp hepatopancreas and shrimp mortality relative to the amount of AgNP demonstrated to be effective against this pathogen.

Antibacterial activity of silver nanoparticles against antibiotic‐resistant Aeromonas veronii infections in Nile tilapia, Oreochromis niloticus (L.), in vitro and in vivo assay

“…Similarly, the use of AgNPs via either immersion or I/P (Intraperitoneal injection) injection in the early stages of A. salmonicida infection in rainbow trout slowed disease progression and reduced mortality (Shaalan et al, 2018). Oral administration of AgNPs for 24 days in Litopenaeus vannamei showed therapeutic benefits against rickettsial infections and increased survival to 100% (Acedo‐Valdez et al, 2017). AgNPs might thus be a suitable alternative to antibiotics for treating bacterial infections affecting fish and shellfish (Das et al, 2020; El‐Adawy et al, 2020; Mahanty et al, 2013).…”

Evaluation of a new Argovit as an antiviral agent included in feed to protect the shrimpLitopenaeus vannameiagainst White Spot Syndrome Virus infection

“…Several studies have evaluated the effectiveness of AgNPs in aquatic diseases control because to their antimicrobial potential against bacteria, virus and parasites (Barakat, El-Sayed & Gohar, 2016;Morales-Covarrubias et al, 2016;Acedo-Valdez et al, 2017;Pimentel-Acosta et al, 2019). However, this is the first work that examine the efficacy of Argovit-4 (AgNPsbased formulation) as a potential antiviral additive in feed to protect the health of shrimp L. vannamei against WSSV.…”