Antibacterial effect of residual bacitracin during culturing from root canal

Grossman, Louis I.; Parris, Leonard; Cobe, Herbert M.

doi:10.1016/0030-4220(57)90169-x

Cited by 9 publications

(2 citation statements)

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“…DISCUSSION From the data, two facts may be deduced, namely, that a residual antimicrobial activity was present and that the activity decreased as the canal surface was dried Vol. 42,No. 2 with absorbent points.…”

Section: Resultsmentioning

confidence: 96%

Residual Antimicrobial Activity of Camphorated Chlorphenol

Grossman

1963

J Dent Res

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Grossman' has pointed out the need for inactivating penicillin by means of penicillinase when taking a sample for culturing from a root canal, in order to rule out the possibility of obtaining a false negative culture. It is conceivable that both microorganisms and the antibiotic could be transferred from the root canal to the culture medium and that the antibiotic could destroy the few microorganisms inoculating the culture medium.Grossman, Parris, and Cobe2 have reported on the possible antibacterial effect of residual bacitracin, a component of the polyantibiotic PBSC (penicillin, bacitracin, streptomycin, and caprylate sodium in a silicone fluid vehicle). While the other components of the polyantibiotic can be inactivated, there is no known inactivator for bacitracin. It has been found that, of the 88 cases studied, only 2 showed a residual antibacterial effect from bacitracin.In discussing the addition of an inactivating agent to the culture medium with the object of neutralizing any antimicrobial activity that might be carried over from the medicament, Grossman' has stated that "no neutralizing agent had been added to the culture medium in the control cases in which the older medicaments such as monochlorphenol, beechwood creosote, etc., had been used." It has been assumed for a number of years, and it has also been stated in dental literature,3 4 that the non-antibiotic root canal medicaments do not need to be inactivated either because they exert no antimicrobial effect after being sealed in the root canal for 2 or 3 days or because they are so diluted by the culture medium that they no longer exert an antimicrobial effect. On the basis of the present studv, we found that this is an erroneous assumption. MATERIALS AND METHODSStudy A.-Agar plates were inoculated with mouth organisms according to the procedure described by Grossman and Appleton,5 as follows: 25 ml. of tap water were put into a sterile Erlenmeyer flask, and 3 ml. of sterile nutrient broth were added to preserve the viability of the organisms. About 10 ml. of this solution were taken into the mouth and swished forcibly back and forth around the teeth. This was continued for 1 minute, and the resultant bacterial suspension was returned to the flask. The flask was shaken by hand to distribute the organisms evenly before each inoculation. Ten milliliters of nutrient agar (pH 7.6) were liquefied, cooled to 450 C., and 1 ml. of

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Section: Resultsmentioning

confidence: 96%

Residual Antimicrobial Activity of Camphorated Chlorphenol

Grossman

1963

J Dent Res

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“…Buchbinder and Bartels [65] in 1951, along with Bender and Seltzer [66] in 1954 also registered their concern regarding the occurrence of false negative cultures that resulted with the transference of the medicaments (primarily streptomycin and chloramphenicol) into the culture media, and cautioned that the bacterial culture method of evaluation should be held in abeyance until some agent appears that promises to neutralize the effect of the medicaments in a medium. In particular, bacitracin was identified by Grossman as the main culprit in this process [67] and he recommended an alteration in the sampling technique that consisted of a least the use of three paper points to remove the paste prior to the final sampling of the root canal.…”

Section: Transitional Perspectivesmentioning

confidence: 99%

Historical and Contemporary Perspectives on the Microbiological Aspects of Endodontics

Gutmann

Manjarrés

2018

Dentistry Journal

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The microbiota of the oral cavity plays a significant role in pulpal and periapical diseases. Historically, 100 years ago little was known on microbiota, but after a century of investigations, only now can many of the intimate secrets of microbial growth, expansion, persistence, communal activities, and virulence be revealed. However, with the capabilities of the microbiota for mutation, quorum sensing, and information transference, researchers are hard-pressed to keep up with both the changes and challenges that an amazingly wide range of bacterial species pose for both the scientist and clinician. Fortunately, the development and expansion of a vast array of molecular biological investigative techniques have enabled dentistry and its associated medical fields to attempt to keep pace with the wide and fascinating world of oral microbiology.

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A clinical study of the use of antibiotic sensitivity testing in endodontics

Goldman

Pearson

1962

Oral Surgery, Oral Medicine, Oral Pathology

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Antibacterial effect of residual bacitracin during culturing from root canal

Cited by 9 publications

References 5 publications

Residual Antimicrobial Activity of Camphorated Chlorphenol

Residual Antimicrobial Activity of Camphorated Chlorphenol

Historical and Contemporary Perspectives on the Microbiological Aspects of Endodontics

A clinical study of the use of antibiotic sensitivity testing in endodontics

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