Antibacterial Effectiveness of Selected Moroccan Essential Oils Against the Highly Virulent Jp2 Clone of Aggregatibacter Actinomycetemcomitans

Abstract

Objective: Aggregatibacter actinomycetemcomitans (Aa) serotype b JP2 clone is a highly virulent strain, considered as a major etiologic agent in aggressive periodontitis in patients of African descent, such as Moroccan adolescents. Antibiotics have been and continue to be the only effective treatment of periodontal infections caused by this periodontal bacterium. However, today there is enough scientific evidence on the existence of an increased resistance of oral bacteria to antibiotics. Therefore, the search for new natural agents, that are safe and effective, such "essential oils," has become a necessity. The present study was conducted to evaluate the in vitro antibacterial activities of three selected essential oils from Moroccan aromatic medicinal plants (Origanum compactum, Thymus vulgaris and Cymbopogon martinii) against clinical Moroccan isolate of Aa JP2 strain.Methods: Antibacterial activity of essential oils was investigated using agar well diffusion method, then measured using broth microdilution method.Results: All the selected essential oils exhibited significant antibacterial activity on the highly pathogenic JP2 strain of Aa. Essential oil of Origanum compactum was found to be the most effective with a minimum inhibitory concentration (MIC) value of 0.03% (v/v) and a minimum bactericidal concentration value (MBC) of 0.07%. Conclusion:The present findings indicate the possibility of exploiting these essential oils as potential antimicrobial agents in treatment of aggressive periodontitis associated to this pathogen.

Histological Examination of Lemongrass Resorbable Dressing on Gingival Healing after Gingivectomy in Rats

“…The results showed the superior properties of the essential oil and suggested the use of the oil with other antibiotics against resistant bacteria. [42][43][44] The mechanism of action suggested is that lemongrass oil and its components induced intracellular leakage, as well as ultrastructural changes in bacterial cells, as it was shown by electron micrographs that a hole occurring on the surface of bacterial cell treated with citral, a main component of lemongrass, at a concentration of 4 μL/mL. 45 Another beneficial effect of lemongrass essential oil is its anti-inflammatory, as well as antifungal, properties.…”

Phytochemical Screening of Artocarpus Hirsutus and Its Antimicrobial Potential

“…Nystatin antibiotic was used as a control. Then, the fungal plates were incubated at 37°C for 24 hrs [18].…”

Exploring the Bioactive Potential of Moroccan Lemongrass (Cymbopogon citratus): Investigations on molecular weight distribution, antioxidant and antimicrobial potentials

“…In Morocco, investigations concerning the chemical composition and biological properties of C. citratus are fragmentary [43][44][45]. Only one study had addressed the chemical C. citratus essential oils composition [44].…”

“…Only one study had addressed the chemical C. citratus essential oils composition [44]. Similarly, one study has tested the EOs inhibition effect against Aggregatibacter actinomycetemcomitans virulent strains of [43]. Therefore, more investigations are needed to determine the antioxidant activities and phenolic and flavonoid contents of varieties of C. citratus that are grown in Morocco so far from its native area (Asia).…”