Antibacterial eﬀects and microarray-based molecular mechanisms of trans-cinnamaldehyde against Porphyromonas gingivalis

Gan, Ning; Fang, Yingjing; Weng, Weimin; Jiao, Ting; Yu, Weiqiang

doi:10.1016/j.heliyon.2023.e23048

Cited by 2 publications

(2 citation statements)

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“…28,31,42 Recently, the microarray-based bioinformatics analysis discovered that CA suppressed the microbial activity of P. gingivalis by disruption of physiological metabolism. 43 A proteomics approach was performed to investigate the antibacterial mechanism of CA against E. coli, and the results suggested aldehyde toxicity, acid stress, oxidative stress, interference with carbohydrate metabolism, energy metabolism, and protein translation as the bactericidal mechanism. 42 Therefore, as a kind of oral bacterium, the mechanism of CA acting on S. mutans may be similar to the previous studies of oral pathogenic bacteria above, on the carbohydrate metabolism.…”

Section: Discussionmentioning

confidence: 99%

“…The antibacterial mechanisms of CA are mainly considered to be interfering with energy metabolism, anti-quorum sensing, changing membrane lipid composition, and inhibiting ATPase. ,, Recently, the microarray-based bioinformatics analysis discovered that CA suppressed the microbial activity of P. gingivalis by disruption of physiological metabolism . A proteomics approach was performed to investigate the antibacterial mechanism of CA against E.…”

Section: Discussionmentioning

confidence: 99%

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Trans-Cinnamaldehyde Inhibition of Pyruvate Dehydrogenase: Effects on Streptococcus mutans Carbohydrate Metabolism

Zhang,

Mu,

Wang

et al. 2024

J. Proteome Res.

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Dental caries is a chronic oral infectious disease, and Streptococcus mutans (S. mutans) plays an important role in the formation of dental caries. Trans-cinnamaldehyde (CA) exhibits broad-spectrum antibacterial activity; however, its target and mechanism of action of CA on S. mutans needs to be further explored. In this study, it was verified that CA could inhibit the growth and biofilm formation of S. mutans. Further proteomic analysis identified 33, 55, and 78 differentially expressed proteins (DEPs) in S. mutans treated with CA for 1, 2, and 4 h, respectively. Bioinformatics analysis showed that CA interfered with carbohydrate metabolism, glycolysis, pyruvate metabolism, and the TCA cycle, as well as amino acid metabolism of S. mutans. Protein interactions suggested that pyruvate dehydrogenase (PDH) plays an important role in the antibacterial effect of CA. Moreover, the upstream and downstream pathways related to PDH were verified by various assays, and the results proved that CA not only suppressed the glucose and sucrose consumption and inhibited glucosyltransferase (GTF) and lactate dehydrogenase (LDH) activities but also decreased the ATP production. Interestingly, the protein interaction, qRT-PCR, and molecular docking analysis showed that PDH might be the target of CA to fight S. mutans. In summary, the study shows that CA interferes with the carbohydrate metabolism of bacteria by inhibiting glycolysis and the tricarboxylic acid (TCA) cycle via binding to PDH, which verifies that PDH is a potential target for the development of new drugs against S. mutans.

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