Antibacterial effects of Schisandra chinensis extract on Staphylococcus aureus and its application in food

Abstract

Schisandra chinensis (Turcz.) Baill is a member of the Magnoliaceae and is used in traditional medicine. Its extracts have antibacterial activity against foodborne pathogens. In present study, the antibacterial properties of Sc. chinensis were tested on S.aureus. An in vitro antibacterial assay showed that Sc. chinensis extracts (SCE) inhibited the growth of test bacteria at a MBC of 18mg mL−1. SCE demonstrated strong antibacterial activity in barley soup system. Treated by SCE, the growth curves of S.aureus exhibited extended lag phases and abbreviated log phases, S. aureus presented with wrinkled and withered surfaces and surrounded by soluble substances probably consisting of leaked intracellular materials. Further, S. aureus released the periplasmic enzyme (AKPase) and increased activities of membrane‐bound Na+/K+/ Ca++‐ATPase. Therefore, SCE can damage S. aureus cell membranes and walls. Moreover, SCE decreased dehydrogenase and total ATPase activities, and intracellular protein contents, even interacted with DNA by groove binding. Practical applications In a barley soup model food system, SCE possessed a high level of antibacterial activity and significantly reduced total viable bacterial counts. This study laid the theoretical foundation for the application of Schisandra chinensis extract in preventing the growth of foodborne pathogens.

Chromatographic and Biological Screening of Chosen Species of Schisandraceae Family: Schisandra chinensis, S. rubriflora, S. sphenanthera, S. henryi and Kadsura japonica

“…(against Gram‐negative Escherichia coli ) [28] and Song et al. (against Gram‐positive Staphylococcus aureus [29] . The S. chinensis fruit activity against S. aureus was also confirmed in our laboratory (not published results).…”

“…[3] Small zones of inhibition at the bottom of the plate were found also for leaf extracts of S. chinensis and S. henryi, as well as for the male and female leaf extracts of S. rubriflora. High antibacterial properties of the S. chinensis fruit extract were also found by Cui et al (against Gram-negative Escherichia coli) [28] and Song [29] The S. chinensis fruit activity against S. aureus was also confirmed in our laboratory (not published results).…”

Natural Extracts as Inhibitors of Microorganisms and Lipid Oxidation in Emulsion Sausage during Storage

“…Schisandra chinensis has antimicrobial activity, and its extracts can inhibit L. monocytogenes , E. coli , and Salmonella enteritidis ( Choi et al, 2013 ). E. coli is inhibited by the release of cellular contents, resulting in destruction of the bacterial cells, following incubation with Schisandra chinensis extracts ( Song et al, 2018 ). Furthermore, Ecklonia cava , a seaweed, is also known to have antimicrobial activity against foodborne pathogens, such as Salmonella spp.…”

Nucleic acid strand displacement for indirect determination of foodborne bacteria by capillary electrophoresis and its application in antagonism and bacteriostasis studies

“…Some traditional Chinese medicines have good abilities of bacteriostasis for bacteria, including foodborne bacteria [23]. C. chinensis, S. chinensis, and honeysuckle are frequently applied in the treatment of the infection of bacteria [24][25][26]. In order to study the antibacterial effects of the medicines on different bacteria, the bacteria were inhibited by the three medicines and then determined by the developed method.…”