Antibacterial Effects of Lactoferrin and a Pepsin-Generated Lactoferrin Peptide Against Helicobacter pylori in vitro

Yamazaki, Natsuko; Yamauchi, Koji; Kawase, Kouzou; Hayasawa, Hirotoshi; Nakao, Koji; Imoto, Ichiro

doi:10.1007/bf02490180

Cited by 13 publications

(20 citation statements)

References 28 publications

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“…20 In contrast to bLF, lactoferricin B has bactericidal activity against H. pylori at a concentration of more than 0.1 mg/ml (3 ¥ 10 -5 mol/l), which is equivalent to 2.6 mg/ml bLF. 11 We also reported previously that bLF and its degraded products were recovered in gastric juice at a total concentration of 5.7 ¥ 10 -5 mol/l after administration of 2 g, i.e., 200 ml of 10 mg/ ml bLF (1.2 ¥ 10 -4 mol/l), and the gastric contents contained lactoferricin B at a concentration of 5.6 ¥ 10 -6 mol/l, which is five times less than the bactericidal concentration under these conditions. 20 Lactoferricin B, but not bLF, has the capacity to inhibit urease of H. pylori at a concentration of approximately 3 mg/ml (1 ¥ 10 -3 mol/l).…”

Section: Discussionmentioning

confidence: 97%

“…The estimated bLF concentration was slightly higher than the inhibitory concentration of bLF in vitro, i.e., 1.0 mg/ml. 11 There have been two reports about the effect of oral administration of recombinant human LF against H. pylori infection. Opekun et al administrated 250 mg or 1 g recombinant human LF to 12 subjects five times in a 24-h period and found no significant change in the UBT value.…”

Section: Discussionmentioning

confidence: 99%

“…20 Lactoferricin B, but not bLF, has the capacity to inhibit urease of H. pylori at a concentration of approximately 3 mg/ml (1 ¥ 10 -3 mol/l). 11 The inhibition, however, might not affect UBT results in the present study, because lactoferricin B in the stomach was assumed to be far below the inhibitory concentration to urease, and UBT was performed at least 4 h after administration of bLF.…”

Section: Discussionmentioning

confidence: 99%

“…9,10 Bovine lactoferrin (bLF) has been demonstrated to have antibacterial activity to H. pylori in vitro. 11 The administration of bLF to H. pylori-infected mice resulted in marked decrease of bacterial colonization, attachment, and inflammation irrespective of iron-free or partially iron-saturated preparations. 12,13 In humans with H. pylori infection, Di Mario et al showed that bLF combined in a triple eradication regimen (rabeprazole, clarithromycin, and tinidazole) significantly improved the eradication rate.…”

Section: Introductionmentioning

confidence: 99%

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Bovine lactoferrin is effective to suppress Helicobacter pylori colonization in the human stomach: A randomized, double-blind, placebo-controlled study

Okuda

Nakazawa

Yamauchi

et al. 2005

Journal of Infection and Chemotherapy

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Bovine lactoferrin is effective to suppress Helicobacter pylori colonization in the human stomach: A randomized, double-blind, placebo-controlled study

Okuda

Nakazawa

Yamauchi

et al. 2005

Journal of Infection and Chemotherapy

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“…pylori ATCC 43504 and 43629 were obtained from the American Type Culture Collection (Rockville, MD, USA). Ten different human clinical isolates of H. pylori isolated from gastric tissue of H. pylori-positive patients treated at Mie University Hospital (Mie, Japan), of which four isolates had been used in a previous study [32], were used. The bacteria were grown either on Trypticase Soy Agar (Becton Dickinson) supplemented with de®brinated horse blood (Nihon Biosupp.…”

Section: Bacteria and Growth Conditionsmentioning

confidence: 99%

Susceptibility of Helicobacter pylori and its urease activity to the peroxidase-hydrogen peroxide-thiocyanate antimicrobial system

Shin¹,

Yamauchi²,

Teraguchi³

et al. 2002

Journal of Medical Microbiology

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The susceptibility of Helicobacter pylori to the antimicrobial system involving lactoperoxidase, hydrogen peroxide and thiocyanate was investigated. The inhibitory effect of the system on the urease activity of H. pylori, which plays a role in its colonisation of the stomach, was also investigated. Twelve H. pylori strains examined, including 10 clinical isolates, were all inhibited by the peroxidase system in brain-heart infusion broth supplemented with fetal calf serum, but to different extents. The killing effect was observed within 3 h. Although bacterial viability recovered afterwards, there was still a clear difference between cultures incubated in the presence of the complete system and control cultures incubated in the absence of lactoperoxidase, after incubation for 24 h. The urease activity and viability of H. pylori were both inactivated by this system in phosphate buffer. These effects were dependent on the concentrations of both lactoperoxidase and hydrogen peroxide and were abolished by the addition of cysteine. Furthermore, these effects were observed when bovine lactoperoxidase was replaced by recombinant human lactoperoxidase or native or recombinant human myeloperoxidase. The peroxidase system found in saliva and milk may contribute to the host defence against H. pylori infection and inhibition of transmission via the oral route.

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The Innate Immune Glycoprotein Lactoferrin Represses the Helicobacter pylori cag Type IV Secretion System

Haley

Francis

et al. 2021

ChemBioChem

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Chronic infection with Helicobacter pylori increases risk of gastric diseases including gastric cancer. Despite development of a robust immune response, H. pylori persists in the gastric niche. Progression of gastric inflammation to serious disease outcomes is associated with infection with H. pylori strains which encode the cag Type IV Secretion System (cag T4SS). The cag T4SS is responsible for translocating the oncogenic protein CagA into host cells and inducing pro‐inflammatory and carcinogenic signaling cascades. Our previous work demonstrated that nutrient iron modulates the activity of the T4SS and biogenesis of T4SS pili. In response to H. pylori infection, the host produces a variety of antimicrobial molecules, including the iron‐binding glycoprotein, lactoferrin. Our work shows that apo‐lactoferrin exerts antimicrobial activity against H. pylori under iron‐limited conditions, while holo‐lactoferrin enhances bacterial growth. Culturing H. pylori in the presence of holo‐lactoferrin prior to co‐culture with gastric epithelial cells, results in repression of the cag T4SS activity. Concomitantly, a decrease in biogenesis of cag T4SS pili at the host‐pathogen interface was observed under these culture conditions by high‐resolution electron microscopy analyses. Taken together, these results indicate that acquisition of alternate sources of nutrient iron plays a role in regulating the pro‐inflammatory activity of a bacterial secretion system and present novel therapeutic targets for the treatment of H. pylori‐related disease.

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Antibacterial Effects of Lactoferrin and a Pepsin-Generated Lactoferrin Peptide Against Helicobacter pylori in vitro

Cited by 13 publications

References 28 publications

Bovine lactoferrin is effective to suppress Helicobacter pylori colonization in the human stomach: A randomized, double-blind, placebo-controlled study

Bovine lactoferrin is effective to suppress Helicobacter pylori colonization in the human stomach: A randomized, double-blind, placebo-controlled study

Susceptibility of Helicobacter pylori and its urease activity to the peroxidase-hydrogen peroxide-thiocyanate antimicrobial system

The Innate Immune Glycoprotein Lactoferrin Represses the Helicobacter pylori cag Type IV Secretion System

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