Antibacterial effects of the lectin from pomegranate sarcotesta (PgTeL) against
            <i>Listeria monocytogenes</i>

Silva, P.M.; Silva, J.N.O.; Silva, B.R.; Ferreira, Gustavo Ramos Salles; Gaião, Wyndly Daniel Cardoso; Recio, Miguel Villoria; Gonçalves, Gabriel Gazzoni Araújo; Rodrigues, Cláudio Gabriel; Medeiros, Paloma Lys de; Brayner, Fábio André; Alves, Luíz Carlos; Larsen, Mona; Ingmer, Hanne; Napoleão, Thiago Henrique; Paiva, Patrícia Maria Guedes

doi:10.1111/jam.14978

Cited by 13 publications

(2 citation statements)

References 33 publications

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“…In the present study, the MIC of moringin against L. monocytogenes was 400 μM (0.124 mg/ml; Table 2 ). Compared to that of phenyllactic acid (1.25 mg/ml; Ning et al, 2017 ) and the lectin from pomegranate sarcotesta (0.0125 mg/ml; Silva et al, 2021 ), moringin showed a moderate inhibitory effect against L. monocytogenes . Moringin is one of the isothiocyanates.…”

Section: Discussionmentioning

confidence: 92%

Multi-Target Antibacterial Mechanism of Moringin From Moringa oleifera Seeds Against Listeria monocytogenes

Wen

et al. 2022

Front. Microbiol.

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Moringin [4-(α-L-rhamnosyloxy) benzyl isothiocyanate] is an isothiocyanate from Moringa oleifera seeds. It is the bioactivated form of the glucosinolate precursor glucomoringin with various health benefits. However, few studies have examined the antibacterial activity of moringin. This study aimed to investigate the antimicrobial activity and mechanism of moringin against Listeria monocytogenes. The minimum inhibitory concentration (MIC), and growth curves were used to evaluate the bacteriostatic effect of moringin against L. monocytogenes. Transcriptome analysis by RNA sequencing was performed to elucidate the underlying mechanism of moringin against L. monocytogenes. The transcriptome results were validated. The results showed that moringin inhibited the growth of L. monocytogenes with a MIC of 400 μM. RNA sequencing results showed that the differences in the expression of genes related to the cell wall and membrane biosynthesis, phosphotransferase system (PTS), oxidative stress, energy metabolism, and DNA binding were significantly affected. As with the transcriptome results, the results of the mechanism verification found that moringin damaged the integrity of the cell wall and cell membrane, stimulated oxidative stress, interfered with energy metabolism and DNA replication, and finally led to the death of L. monocytogenes. The present study provides evidence that moringin exhibits strong antimicrobial activity against L. monocytogenes and insight into its potential mechanism.

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Section: Discussionmentioning

confidence: 92%

Multi-Target Antibacterial Mechanism of Moringin From Moringa oleifera Seeds Against Listeria monocytogenes

Wen

et al. 2022

Front. Microbiol.

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“…Indeed, agglutination assays showed that Lm reacts to different plant lectins in a strain-specific manner (Facinelli et al, 1998;Slifkin & Doyle, 1990). However, lectins of Canavalia ensiformis and Punica granatum have antibiofilm activities against Lm and other bacteria (Jin et al, 2019;Silva et al, 2021). This suggests that lectins of some plant species may limit adhesion to their surface.…”

Section: Lm Attachment To Plantsmentioning

confidence: 99%

Plants as a realized niche for Listeria monocytogenes

et al. 2021

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Listeria monocytogenes is a human pathogen. It is the causative agent of listeriosis, the leading cause of bacterial-linked foodborne mortality in Europe and elsewhere. Outbreaks of listeriosis have been associated with the consumption of fresh produce including vegetables and fruits. In this review we summarize current data providing direct or indirect evidence that plants can serve as habitat for L. monocytogenes, enabling this human pathogen to survive and grow. The current knowledge of the mechanisms involved in the interaction of this bacterium with plants is addressed, and whether this foodborne pathogen elicits an immune response in plants is discussed.

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