Antibacterial efficacy of a fucoidan fraction (Fu-F2) extracted from Sargassum polycystum

Palanisamy, Subramanian; Vinosha, Manoharan; Rajasekar, Periyannan; Anjali, Ravichandran; Ganesan, S.; Marudhupandi, Thangapandi; Selvam, S.; Prabhu, Nagesh; You, SangGuan

doi:10.1016/j.ijbiomac.2018.12.070

Cited by 65 publications

(45 citation statements)

References 44 publications

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“…In present work, significant nucleic acid release of ETEC K88 was observed after being exposed to depolymerized sulfated galactans. This result is consistent with earlier reports, in which the sulfated polysaccharides from Laminaria japonica and Sargassum polycystum also display a destructive effect on the membrane permeability for Gram-negative and -positive bacteria, including E. coli, S. aureus, and Pseudomonas aeruginosa [6,21]. However, at longer times, the amount of 260 nm absorbing material tended to decrease with time.…”

Section: Discussionsupporting

confidence: 93%

“…In addition to molecular weight, charge density, and sulfate content, the monosaccharide unit plays an important role in the biological actions of polysaccharides [21]. In present research, no anti-ETEC K88 activity was observed for dextran sulfate even at 10.0 mg/mL.…”

Section: Discussioncontrasting

confidence: 57%

“…Sulfated polysaccharides usually exhibit polyanionic property due to their abundant contents of sulfate group and uronic acid [6]. Binding and reacting with the glycoprotein-receptors on the bacterial cell surface are suggested as the antibacterial mechanisms of polysaccharides [21]. Therefore, the charge property of sulfated galactans may affect its interaction with bacteria.…”

Section: Discussionmentioning

confidence: 99%

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Inhibitory Effect of Depolymerized Sulfated Galactans from Marine Red Algae on the Growth and Adhesion of Diarrheagenic Escherichia coli

Liu

et al. 2019

Marine Drugs

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Active polysaccharides as safe and natural polymers against bacterial diarrhea have been reconsidered as an alternative to antibiotics. This work investigated the inhibiting effect of depolymerized sulfated galactans from Eucheuma serra and Gracilaria verrucosa on the growth and adhesion of diarrheagenic enterotoxigenic Escherichia coli (ETEC) K88. Results showed that the sulfated polysaccharides with molecular weight distribution ≤20.0 kDa exhibited antibacterial activity against ETEC K88. A structure–activity study revealed that the anti-ETEC K88 activity of sulfated polysaccharides is strictly determined by their molecular weight distribution, sulfate group content, and monosaccharide composition. In addition, the promoted nucleic acid release and the fluorescence quenching of membrane proteins were observed after the treatment with selected polysaccharides. Scanning electron microscopy further confirmed that the depolymerized sulfated galactans can effectively inhibit ETEC K88 adhesion. In conclusion, depolymerized sulfated galactans exhibited an inhibitory effect on the growth and adhesion of ETEC K88.

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Section: Discussionsupporting

confidence: 93%

Section: Discussioncontrasting

confidence: 57%

Section: Discussionmentioning

confidence: 99%

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Inhibitory Effect of Depolymerized Sulfated Galactans from Marine Red Algae on the Growth and Adhesion of Diarrheagenic Escherichia coli

Liu

et al. 2019

Marine Drugs

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“…This method is routinely applied in laboratory medicine to determine the susceptibility profile of clinical isolates to conventional antibiotics. Moreover, it is also successfully used for the screening and investigation of plant extracts, quantum dots, and nanoparticles [64][65][66][67][68][69][70].…”

Section: Standard Methods Used For the Determination Of Antimicrobialmentioning

confidence: 99%

Methods Used for the Eradication of Staphylococcal Biofilms

et al. 2019

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Staphylococcus aureus is considered one of the leading pathogens responsible for community and healthcare-associated infections. Among them, infections caused by methicillin-resistant strains (MRSA) are connected with ineffective or prolonged treatment. The therapy of staphylococcal infections faces many difficulties, not only because of the bacteria’s resistance to antibiotics and the multiplicity of virulence factors it produces, but also due to its ability to form a biofilm. The present review focuses on several approaches used for the assessment of staphylococcal biofilm eradication. The methods described here are successfully applied in research on the prevention of biofilm-associated infections, as well as in their management. They include not only the evaluation of the antimicrobial activity of novel compounds, but also the methods for biomaterial functionalization. Moreover, the advantages and limitations of different dyes and techniques used for biofilm characterization are discussed. Therefore, this review may be helpful for those scientists who work on the development of new antistaphylococcal compounds.

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“…Fucoidan, fucose containing polysaccharides (FPs) belongs to a heterogeneous polysaccharide group obtained mainly from the extracellular medium with cellulose, hemicellulose, and alginate in brown seaweeds and primarily segregated by Kylin in 1993 [1,2]. In recent years FPs has acquired much curiosity in functional food and pharmaceutical industries because of its abundant bioactive properties such as anti-cancer/anti-tumor [3,4], anti-viral, anti-oxidant, anti-coagulant, anti-diabetic, anti-obesity, anti-bacterial [5] and therapeutic potential against injuries [6,7]. Different studies exhibited that the structure of FPs varies from each other due to the position of glycosidic bonds, branches of the chain, relative molecular weight and sulfate group substitutions, which are caused by extraction methods, type of species, region of growth, time of harvesting and other environmental and geographical factors [8,9].…”

Section: Introductionmentioning

confidence: 99%

Characterization of L-fucose isomerase from Paenibacillus rhizosphaerae to produce L-fuculose from hydrolyzed fucoidan and commercial fucose

Iqbal¹,

Riaz²,

Mahmood³

et al. 2019

Preprint

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Background L-fuculose is an expensive and rare sugar used against different kinds of diseases such as HIV, anti-cancer, anti-viral, Hepatitis-B, human lysosomal disease (fucosidosis), and cardio-protective drugs. The enzymatic way of converting L-fucose into L-fuculose would be an effective method with great industrial applications. The purpose of this research is to introduce a high production of L-fuculose from cheap and natural sources (fucoidan) and commercially source (Sigma-Aldrich) by a recombinant enzyme L-fucose isomerase from Paenibacillus rhizosphaerae (Pa-LFI).Results Fucose containing polysaccharide (FPs) called fucoidan was extracted, hydrolyzed and characterized by U. pinnatifida for enzymatic production of L-fuculose. The FPs provide 35.9% of fucose along with few other monosaccharides. Pa-LFI was characterized and purified with a single band at 65 kDa. It showed an activity of 104.5 U mg -1 and exhibited as a hexamer with native molecular mass 396 kDa. The maximum activity for recombinant Pa-LFI was detected at pH 6.5 and 50 °C in 1 mM of Mn 2+ . The melting temperature observed 75 °C and half-life at 50 °C was 12.6 h. The isomerizing activity of Pa-LFI with aldose substrate (L-fucose) was higher exposing K m , k cat and k cat / K m 86.2 mM, 32831 min -1 and 335 min -1 mM -1 respectively. The conversion ratio of L-fuculose from 100 g L -1 of FPs and commercial fucose after the equilibrium state was about 6% (5.6 g L -1 ) and 30% (30.2 g L -1 ) respectively.Conclusion Pa-LFI catalyzed the reaction to convert L-fucose into L-fuculose. The enzyme will be helpful in the production of L-fuculose with an efficient and simple method without producing any by-product.

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Antibacterial efficacy of a fucoidan fraction (Fu-F2) extracted from Sargassum polycystum

Cited by 65 publications

References 44 publications

Inhibitory Effect of Depolymerized Sulfated Galactans from Marine Red Algae on the Growth and Adhesion of Diarrheagenic Escherichia coli

Inhibitory Effect of Depolymerized Sulfated Galactans from Marine Red Algae on the Growth and Adhesion of Diarrheagenic Escherichia coli

Methods Used for the Eradication of Staphylococcal Biofilms

Characterization of L-fucose isomerase from Paenibacillus rhizosphaerae to produce L-fuculose from hydrolyzed fucoidan and commercial fucose

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