Antibacterial Efficacy of Green Silver Nanoparticles Against Bacteria Isolated from Calf Diarrhoea

Zeedan, Gamil Sayed Gamil; Abdalhamed, Abeer M.; Ibrahim, Eman S.; El-Sadawy, Hanan A.

doi:10.3923/aje.2018.65.73

Cited by 8 publications

(6 citation statements)

References 19 publications

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“…Finally, the zone of growth inhibition was compared using standards provided elsewhere [26] to identify the resistant isolates. Isolates showing resistance to three or more different classes of antibiotics were defined as MDR [27].…”

Section: Antimicrobial Sensitivity Test (Ast)mentioning

confidence: 99%

Therapeutic effect of biosynthetic gold nanoparticles on multidrug-resistant Escherichia coli and Salmonella species isolated from ruminants

et al. 2021

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Background and Aim: Multidrug-resistant (MDR) pathogenic microorganisms have become a global problem in ruminants as a result of the intensive use of antibiotics, causing the development of resistance among gut microbiota. The antibiotic-resistant microorganisms can be transferred from diseased animals to humans. This study aimed to determine the prevalence of MDR Escherichia coli and Salmonella spp. isolated from cattle, buffaloes, sheep, and goats suffering from respiratory signs, diarrhea, and mastitis and to screen the antibiotic sensitivity of selected isolated bacteria. It also detected antibiotic-resistance genes by polymerase chain reaction (PCR), produced green gold nanoparticles (AuNPs) using plant extracts (Artemisia herba-alba and Morus alba), and evaluated the antimicrobial activities of these biosynthesized nanoparticles on selected pathogens (E. coli and Salmonella spp.). Materials and Methods: MDR E. coli and Salmonella spp. were investigated using fecal samples (n=408), nasal swabs (n=358), and milk samples (n=227) of cattle, buffaloes, sheep, and goats with or without clinical signs, including respiratory manifestations, pneumonia, diarrhea, and mastitis, from different governorates in Egypt. E. coli and Salmonella spp. were isolated and identified on selective media, which were confirmed by biochemical reactions and PCR. Antimicrobial susceptibility testing against 10 commonly used antibiotics was performed using the Kirby-Bauer disk diffusion method. Antibiotic resistance genes blaTEM, blaSHV, blaOXA, and blaCTX-M were detected by PCR. The antibacterial effect of the biosynthesized AuNPs was evaluated by MIC and well diffusion assay. The biosynthesized AuNPs were also characterized by ultraviolet-visible spectrophotometry and transmission electron microscopy (TEM). Results: Among all fecal samples, the prevalence of E. coli was 18.4% (183/993) and that of Salmonella spp. was 16.7% (66/408), as determined by cultural and molecular tests. All isolates of E. coli and Salmonella spp. were 100% resistant to ampicillin (AM) and amoxicillin and highly resistant to cefoxitin and AM-sulbactam. The total rate of resistance genes in E. coli was 61.2% (112/183), while that in Salmonella was 63.6% (42/66) for pathogens isolated from ruminants with respiratory manifestations, pneumonia, diarrhea, and mastitis. Among the resistance genes, blaTEM had the highest prevalence rate in E. coli (25.9%, 21/81) while blaSHV had the lowest (9.8%, 8/81) in fecal swabs. AuNPs were successfully synthesized using aqueous leaf extract of A. herba-alba and M. alba as bioreducing agents. TEM analysis showed particle size of 10-42 nm for A. herba-alba and M. alba AuNPs. The biosynthesized AuNPs showed antibacterial activity against MDR E. coli and Salmonella spp. Conclusion: Rapid and accurate diagnostic methods are the cornerstone for effective treatment to reduce the risk of antimicrobial-resistant pathogenic microorganisms. This is particularly important for overcoming the increasing rate of MDR in ruminants with respiratory manifestations, pneumonia, diarrhea, and mastitis. This can be complemented by the development of AuNPs synthesized in an environmentally friendly manner AuNPs using natural plant extracts for the treatment of antibiotic-resistant microorganisms.

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Section: Antimicrobial Sensitivity Test (Ast)mentioning

confidence: 99%

Therapeutic effect of biosynthetic gold nanoparticles on multidrug-resistant Escherichia coli and Salmonella species isolated from ruminants

et al. 2021

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“…Recent studies have reported the use of various plant species such as Sesbania grandiflora L. leaf extract, Moringa oleifera , Aloe barbadensis , Calotropis gigantea , Ageratina altissima L., Curcuma longa , and Vitex negundo in the synthesis of TiO2 NPs [ 16 , 17 ]. In recent years, the fear of pan drug resistance (resistance to all antibiotics) has increased, so we are trying to find a new antimicrobial therapy for clinical mastitis (CM) and subclinical mastitis (SCM) caused by MDR S. aureus using green synthesis of metal oxide nanoparticles [ 18 , 19 ].…”

Section: Introductionmentioning

confidence: 99%

Detection of Methicillin-Resistant Staphylococcus aureus in Clinical and Subclinical Mastitis in Ruminants and Studying the Effect of Novel Green Synthetized Nanoparticles as One of the Alternative Treatments

Abdalhamed

Zeedan

Arafa

et al. 2022

Veterinary Medicine International

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Mastitis is an important disease in dairy animals worldwide. Methicillin-resistant Staphylococcus aureus (MRSA) is one of the most common causes of clinical and subclinical intramammary infections. In the current study, we isolated bacteria from 150 mastitic milk samples. Multiplex PCR was used to detect the methicillin resistance genes in S. aureus to detect the occurrence of MRSA isolates. Green synthesized titanium dioxide nanoparticles (TiO2 NPs) using aqueous leaf extracts of Artemisia herb Alba (A. herb Alba TiO2 NPs). The antibacterial efficacy of these nanoparticles was evaluated (in vitro and in vivo) against collected MRSA isolates using the disc diffusion method and SPF rats. Out of 150 mastitic milk samples, the frequency of S. aureus was 38 (25.3%), that of E. coli was 45 (30%), that of Klebsiella spp. Was 7 (4.7%), and that of Streptococcus spp. Was 11 (7.3%). Among 38 positive isolates of S. aureus, MRSA was 16 (42.1%) by antimicrobial sensitivity testing (AST) and 14 (38.8%) by multiplex PCR. The MRSA isolates were shown to have 100% resistance to penicillin and methicillin, 87.5% resistance to gentamicin, 50% resistance to cefoxitin and amoxicillin, and 75% resistance to ampicillin and ampicillin/sublactam with low resistance against erythromycin, ciprofloxacin, tetracycline, and levofloxacin by AST, respectively. A. herb Alba TiO2 NP formation was observed by changing the colour from white to dark green. The UV spectrum revealed absorbance peaks at 240–250 nm, and their sizes ranged from 42–66 nm and 11 to 45 nm by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). ‏A. herb Alba TiO2 NP suspensions were evaluated against MRSA, with the highest zone of inhibition (43 ± 0.45 mm) at a concentration of 40 μg/ml. Hematological parameters and histological examination after oral administration of 20 mg/kg of A. herb Alba TiO2 NPs indicated that A. herb Alba TiO2 NPs can be used as a new antimicrobial against resistant bacteria (MRSA) with consideration of the dose and methods of synthesis of plant-based compounds.

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“…Yew et al (2016) reported that the different sizes of AuNPs successfully inhibited the growth of varying MDR bacteria, including MRSA. Zamek-Gliszczynski et al (2018) mentioned that AgNPs and capsaicin have antibacterial characteristics and could effectively be used to inhibit the growth of MDR-extended-spectrum betalactamases (ESBL) producing E. coli of bovine origin (Thu et al, 2017;Zeedan et al, 2018). The AgNPS has a promising effect on AMR bacteria.…”

Section: Applications Of Nanoparticles For the Prevention And Treatme...mentioning

confidence: 99%

Strategies for Prevention and Control of Multidrug-resistant Bacteria in Ruminants

Zeedan¹,

Abdalhamed²,

Ghazy³

2023

WVJ

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Antibiotics are no longer effective in treating bacterial infections due to antimicrobial drug resistance. Therefore, various alternative strategies have been developed to combat multidrug-resistant (MDR) bacteria. The current review article aimed to shed light on strategies to prevent and control MDR bacteria in ruminants. Due to the development of new resistant bacteria, there is a need for effective treatments and prevention protocols in livestock and humans. With growing antibiotic-resistant organisms, a few antimicrobial medicines will be available to treat the infection when no new drugs are developed. This highlights the importance of looking for other strategies for combating antibiotic-resistant bacteria. In this regard, alternative strategies have been proposed to minimize antimicrobial drug overuse in ruminants. These alternative procedures include alternatives for growth promotion (such as in-feed enzymes, probiotics, prebiotics, synbiotics, and antimicrobial peptides), alternatives for disease prevention (such as vaccines, immune modulators, chicken egg yolk antibodies, farm management, and biosecurity), and alternatives for disease treatment such as plant extracts and phage-therapy to antibiotics. These alternative methods should be safe‎ and efficient without inducing microbial resistance.

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Antibacterial Efficacy of Green Silver Nanoparticles Against Bacteria Isolated from Calf Diarrhoea

Cited by 8 publications

References 19 publications

Therapeutic effect of biosynthetic gold nanoparticles on multidrug-resistant Escherichia coli and Salmonella species isolated from ruminants

Therapeutic effect of biosynthetic gold nanoparticles on multidrug-resistant Escherichia coli and Salmonella species isolated from ruminants

Detection of Methicillin-Resistant Staphylococcus aureus in Clinical and Subclinical Mastitis in Ruminants and Studying the Effect of Novel Green Synthetized Nanoparticles as One of the Alternative Treatments

Strategies for Prevention and Control of Multidrug-resistant Bacteria in Ruminants

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