Antibacterial efficacy of synthesized silver nanoparticles of Microbacterium proteolyticum LA2(R) and Streptomyces rochei LA2(O) against biofilm forming meningitis causing microbes

Abstract

Actinobacteria obtained from the least explored Indian regions were studied for their ability to suppress meningitis-causing bacteria in nanoparticle form. Drug-resistant bacteria and long-term treatment with different medications make meningitis control complicated. Thus, new meningitis drugs are required to combat MDR bacteria. In this study, secondary metabolites isolated from actinomycetes strains, Microbacterium proteolyticum LA2(R) and Streptomyces rochei LA2(O), were employed to synthesize silver nanoparticles (AgNPs) at 37 °C for seven days incubation. UV–Vis spectroscopy, TEM, FTIR, and HPLC studies were used for the confirmation of the synthesis of AgNPs. Furthermore, these NPs demonstrated antibacterial and antibiofilm activities against meningitis-causing bacteria. The average size of LA2(R) and LA2(O) isolated secondary metabolites mediated AgNPs was observed to be 27 ± 1and 29 ± 2 nm by TEM analysis. FTIR study of RAgNPs and OAgNPs revealed that presence of peaks with positions of 1637.17 cm1 and 1636.10 cm1 for C=O amide group appearances in the amide I linkage. These NPs were effective against bacterial pathogens such as S. pneumoniae, H. influenzae, and N. meningitidis and confirmed by their MICs, i.e., 109.4, 120.60, and 138.80 μg/ml of RAgNPs and 105.80, 114.40 and 129.06 μg/ml of OAgNPs, respectively. Additionally, the production of biofilms is impeded by these nanoparticles on S. pneumoniae, H. influenzae, and N. meningitidis by 73.14%, 71.89% and 64.81%, respectively. These findings confirm the potential role of synthesized AgNPs against biofilm forming meningitis causing Multidrug resistance (MDR) microbes.

Surfactin-Conjugated Silver Nanoparticles as an Antibacterial and Antibiofilm Agent against Pseudomonas aeruginosa

“…The antibacterial and antibiofilm activities of these nanoparticles might be due to the interaction of conjugated biomolecules with the bacterial cells and biofilm components. 12 Moreover, silver ions may cause alteration in the permeability of the cell wall/membrane, and also interact with intracellular molecules such as DNA, enzymes, and proteins. 13 The interaction of nanoparticles with extracellular and intracellular components mainly depends upon the surface moiety and/or molecules present on their surfaces.…”

“…Moreover, the synthesized nanoparticles also showed potential antibiofilm activity against all of the tested bacterial strains. The antibacterial and antibiofilm activities of these nanoparticles might be due to the interaction of conjugated biomolecules with the bacterial cells and biofilm components . Moreover, silver ions may cause alteration in the permeability of the cell wall/membrane, and also interact with intracellular molecules such as DNA, enzymes, and proteins …”

Myco-Synthesis of Silver Nanoparticles and Their Bioactive Role against Pathogenic Microbes

“…Many antibiotics have poor membrane transport; these drug-loaded NPs vehicles can penetrate within host cells via endocytosis, making their intracellular penetration easier. Moreover, there is various evidence that exposing microbes to these NPs may improve their tolerance towards antibiotics [11,12]. Hence, silver nanoparticles are one of the promising approaches for the management of pathogenic microbes by preventing bacterial cells' efflux pumps, restoring the bactericidal activity of traditional antibiotics, and decreasing the ability of microorganisms to produce biofilms [6,13,14].…”

Multitargeted Virtual Screening and Molecular Simulation of Natural Product-like Compounds against GSK3β, NMDA-Receptor, and BACE-1 for the Management of Alzheimer’s Disease

“…Natural products and their derivatives were found to have better therapeutic potentials against several metabolic and infectious disorders such as diabetes, oxidative stress, hyperlipidemia, ulcer, neurodegenerative disorders, cancer and microbial infections [20][21][22][23][24][25][26][27][28][29][30][31][32][33][34][35][36][37][38]. Earlier reports explored the therapeutic roles and mechanism of action of polyphenols against age-related neurological disorders [39,40].…”