2016
DOI: 10.1021/jacs.5b11575
|View full text |Cite
|
Sign up to set email alerts
|

Antibacterial Flavonoids from Medicinal Plants Covalently Inactivate Type III Protein Secretion Substrates

Abstract: Traditional Chinese Medicines (TCMs) have been historically used to treat bacterial infections. However, the molecules responsible for these anti-infective properties and their potential mechanisms of action have remained elusive. Using a high-throughput assay for type III protein secretion in Salmonella enterica serovar Typhimurium, we discovered that several TCMs can attenuate this key virulence pathway without affecting bacterial growth. Amongst the active TCMs, we discovered that baicalein, a specific flav… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1

Citation Types

4
64
0
2

Year Published

2017
2017
2024
2024

Publication Types

Select...
8

Relationship

4
4

Authors

Journals

citations
Cited by 101 publications
(70 citation statements)
references
References 49 publications
4
64
0
2
Order By: Relevance
“…and Yersinia spp. Therefore, these systems are quickly emerging as prime targets for the development of new-generation anti-infective drugs (Charro and Mota, 2015; Gu et al, 2015; Tsou et al, 2016). The type III secretion machine (or injectisome) is a complex multi-protein assembly composed of an envelope-embedded structure known as the needle complex (NC) (Kubori et al, 1998), an inner membrane export apparatus (Wagner et al, 2010), and a cytoplasmic platform that energizes the secretion process and selects and sorts substrates for their orderly delivery to the secretion machine (Lara-Tejero et al, 2011) (Fig.…”
Section: Introductionmentioning
confidence: 99%
“…and Yersinia spp. Therefore, these systems are quickly emerging as prime targets for the development of new-generation anti-infective drugs (Charro and Mota, 2015; Gu et al, 2015; Tsou et al, 2016). The type III secretion machine (or injectisome) is a complex multi-protein assembly composed of an envelope-embedded structure known as the needle complex (NC) (Kubori et al, 1998), an inner membrane export apparatus (Wagner et al, 2010), and a cytoplasmic platform that energizes the secretion process and selects and sorts substrates for their orderly delivery to the secretion machine (Lara-Tejero et al, 2011) (Fig.…”
Section: Introductionmentioning
confidence: 99%
“…These machines play an essential role in the pathogenic or symbiotic relationships between the bacteria that encode them and their respective hosts. Consequently, type III secretion systems are rapidly emerging as targets for the development of novel therapeutic and prevention strategies with the potential to combat several important infectious diseases (3)(4)(5). The components of type III secretion machines are highly conserved across bacterial species although the effector proteins that they deliver are customized for the specific bacteria that harbor them (1,6,7).…”
mentioning
confidence: 99%
“…As epigallocatechin gallate (EGCG) is the major polyphenolic components among the tea derived catechins, we compared the activities of EGCG and ECG with previously reported T3SS inhibitors baicalein 12 and INP0007 19 on endogenously secreted SPI-1 T3SS effectors from the growth media of S. Typhimurium (Fig. 1C).…”
Section: Resultsmentioning
confidence: 99%
“…12 Based on these initial findings, we explored additional plant metabolites from other medicinal and dietary sources with proposed anti-infective activities towards Gram-negative bacterial pathogens. Here we show that epigallocatechin-3-gallate (EGCG), a major metabolite from green tea, 17 a previously reported inhibitor of α-synuclein amyloid formation 13, 14 and hepatitis C viral entry 15, 16 , also effectively inhibits S. Typhimurium T3SS and invasion of host cells.…”
Section: Introductionmentioning
confidence: 99%