Antibacterial isoeugenol coating on stainless steel and polyethylene surfaces prevents biofilm growth

Nielsen, Christina Krogsgård; Subbiahdoss, Guruprakash; Zeng, Gang; Salmi, Zakaria; Kjems, Jørgen; Mygind, Tina; Snabe, Torben

doi:10.1111/jam.13634

Cited by 21 publications

(16 citation statements)

References 18 publications

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“…However, the maximum cell count after 48 h for S. aureus was about 2-Log lower than that of P. fluorescens . This result is in agreement with previous studies, which showed that S. aureus biofilms are usually less dense than P. fluorescens ones (Rossoni and Gaylarde, 2000; Nielsen et al, 2018). Nevertheless, S. aureus biofilms could cause cross-contamination via surfaces in the food field.…”

Section: Discussionsupporting

confidence: 94%

Inactivation of Foodborne Bacteria Biofilms by Aqueous and Gaseous Ozone

2018

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In this study, the efficacy of treatments with ozone in water and gaseous ozone against attached cells and microbial biofilms of three foodborne species, Pseudomonas fluorescens, Staphylococcus aureus, and Listeria monocytogenes, was investigated. Biofilms formed on AISI 304 stainless steel coupons from a mixture of three strains (one reference and two wild strains) of each microbial species were subjected to three types of treatment for increasing times: (i) ozonized water (0.5 ppm) by immersion in static condition, (ii) ozonized water under flow conditions, and (iii) gaseous ozone at different concentrations (0.1–20 ppm). The Excel add-in GinaFit tool allowed to estimate the survival curves of attached cells and microbial biofilms, highlighting that, regardless of the treatment, the antimicrobial effect occurred in the first minutes of treatment, while by increasing contact times probably the residual biofilm population acquired greater resistance to ozonation. Treatment with aqueous ozone under static conditions resulted in an estimated viability reduction of 1.61–2.14 Log CFU/cm2 after 20 min, while reduction values were higher (3.26–5.23 Log CFU/cm2) for biofilms treated in dynamic conditions. S. aureus was the most sensitive species to aqueous ozone under dynamic conditions. With regard to the use of gaseous ozone, at low concentrations (up to 0.2 ppm), estimated inactivations of 2.01–2.46 Log CFU/cm2 were obtained after 60 min, while at the highest concentrations a complete inactivation (<10 CFU/cm2) of the biofilms of L. monocytogenes and the reduction of 5.51 and 4.72 Log CFU/cm2 of P. fluorescens and S. aureus respectively after 60 and 20 min were achieved. Considering the results, ozone in water form might be used in daily sanitation protocols at the end of the day or during process downtime, while gaseous ozone might be used for the treatment of confined spaces for longer times (e.g., overnight) and in the absence of personnel, to allow an eco-friendly control of microbial biofilms and consequently reduce the risk of cross-contamination in the food industry.

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Section: Discussionsupporting

confidence: 94%

Inactivation of Foodborne Bacteria Biofilms by Aqueous and Gaseous Ozone

2018

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“…It is worth mentioning that attachment efficiency can be influenced by the characteristics of host surfaces, such as physicochemical interaction forces like hydrophobicity and electrostatic interactions (Gutierrez‐Pacheco et al., 2018; Hayat et al., 2018). A simple isoeugenol coating inhibits the biofilm formation of S. aureus , L. monocytogenes , and P. fluorescens through changing the hydrophobic properties of the surface (Nielsen et al., 2018). Similarly, Sc.…”

Section: Bacterial Biofilmmentioning

confidence: 99%

Antivirulence properties and related mechanisms of spice essential oils: A comprehensive review

Zhang

Gan

Zhang

et al. 2020

Comp Rev Food Sci Food Safe

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In recent decades, reduced antimicrobial effectiveness, increased bacterial infection, and newly emerged microbial resistance have become global public issues, leading to an urgent need to find effective strategies to counteract these problems. Strategies targeting bacterial virulence factors rather than bacterial survival have attracted increasing interest, since the modulation of virulence factors may prevent the development of drug resistance in bacteria. Spices are promising natural sources of antivirulence compounds owing to their wide availability, diverse antivirulence phytochemical constituents, and generally favorable safety profiles. Essential oils are the predominant and most important antivirulence components of spices. This review addresses the recent efforts of using spice essential oils to inhibit main bacterial virulence traits, including the quorum sensing system, biofilm formation, motility, and toxin production, with an intensive discussion of related mechanisms. We hope that this review can provide a better understanding of the antivirulence properties of spice essential oils, which have the potential to be used as antibiotic alternatives by targeting bacterial virulence. K E Y W O R D Sbiofilm, mechanisms, motility, quorum sensing, spice essential oils, toxin 1018

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“…Infections associated with biofilm formation on the surface of implants, medical devices, catheters or food plates represent a major problem in surgical procedures, infection treatment, and recuperation. They constitute a serious risk for patients in the hospital environment [129,130]. Thus, limiting the survival of biofilm-embedded pathogens grants an opening to reduce the number of microbial infections [131].…”

Section: The Employment Of Atomic Force Microscopy In the Investigation Of Microbial Biofilmsmentioning

confidence: 99%

Physics Comes to the Aid of Medicine—Clinically-Relevant Microorganisms through the Eyes of Atomic Force Microscope

et al. 2020

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Despite the hope that was raised with the implementation of antibiotics to the treatment of infections in medical practice, the initial enthusiasm has substantially faded due to increasing drug resistance in pathogenic microorganisms. Therefore, there is a need for novel analytical and diagnostic methods in order to extend our knowledge regarding the mode of action of the conventional and novel antimicrobial agents from a perspective of single microbial cells as well as their communities growing in infected sites, i.e., biofilms. In recent years, atomic force microscopy (AFM) has been mostly used to study different aspects of the pathophysiology of noninfectious conditions with attempts to characterize morphological and rheological properties of tissues, individual mammalian cells as well as their organelles and extracellular matrix, and cells’ mechanical changes upon exposure to different stimuli. At the same time, an ever-growing number of studies have demonstrated AFM as a valuable approach in studying microorganisms in regard to changes in their morphology and nanomechanical properties, e.g., stiffness in response to antimicrobial treatment or interaction with a substrate as well as the mechanisms behind their virulence. This review summarizes recent developments and the authors’ point of view on AFM-based evaluation of microorganisms’ response to applied antimicrobial treatment within a group of selected bacteria, fungi, and viruses. The AFM potential in development of modern diagnostic and therapeutic methods for combating of infections caused by drug-resistant bacterial strains is also discussed.

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Antibacterial isoeugenol coating on stainless steel and polyethylene surfaces prevents biofilm growth

Cited by 21 publications

References 18 publications

Inactivation of Foodborne Bacteria Biofilms by Aqueous and Gaseous Ozone

Inactivation of Foodborne Bacteria Biofilms by Aqueous and Gaseous Ozone

Antivirulence properties and related mechanisms of spice essential oils: A comprehensive review

Physics Comes to the Aid of Medicine—Clinically-Relevant Microorganisms through the Eyes of Atomic Force Microscope

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