Antibacterial mechanism of kojic acid and tea polyphenols against 
            <i>Escherichia coli</i> O157:H7 through transcriptomic analysis

Lin, Yilin; Wang, Ruifei; Li, Xiaoqing; Addo, Keren Agyekumwaa; Fang, Meimei; Zhang, Shaodong; Yu, Yigang

doi:10.26599/fshw.2022.9250063

Cited by 6 publications

(2 citation statements)

References 70 publications

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“…With the development of high-throughput sequencing technologies, transcriptomic sequencing has played a significant role in studying the antimicrobial mechanisms of drugs [ 32 , 33 ]. Here, our transcriptional profiles revealed that the DEGs were primarily involved in bacterial energy metabolism, pyrimidine metabolism, DNA replication, and repair by comparing 5-FU treated groups with control group.…”

Section: Discussionmentioning

confidence: 99%

Deciphering the Antibacterial Mechanisms of 5-Fluorouracil in Escherichia coli through Biochemical and Transcriptomic Analyses

Zhang,

Song,

Yang

et al. 2024

Antibiotics

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The emergence of carbapenem-resistant Gram-negative pathogens presents a clinical challenge in infection treatment, prompting the repurposing of existing drugs as an essential strategy to address this crisis. Although the anticancer drug 5-fluorouracil (5-FU) has been recognized for its antibacterial properties, its mechanisms are not fully understood. Here, we found that the minimal inhibitory concentration (MIC) of 5-FU against Escherichia coli was 32–64 µg/mL, including strains carrying blaNDM-5, which confers resistance to carbapenems. We further elucidated the antibacterial mechanism of 5-FU against E. coli by using genetic and biochemical analyses. We revealed that the mutation of uracil phosphoribosyltransferase-encoding gene upp increased the MIC of 5-FU against E. coli by 32-fold, indicating the role of the upp gene in 5-FU resistance. Additionally, transcriptomic analysis of E. coli treated with 5-FU at 8 µg/mL and 32 µg/mL identified 602 and 1082 differentially expressed genes involved in carbon and nucleic acid metabolism, DNA replication, and repair pathways. The biochemical assays showed that 5-FU induced bacterial DNA damage, significantly increased intracellular ATP levels and the NAD+/NADH ratio, and promoted reactive oxygen species (ROS) production. These findings suggested that 5-FU may exert antibacterial effects on E. coli through multiple pathways, laying the groundwork for its further development as a therapeutic candidate against carbapenem-resistant bacterial infections.

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Section: Discussionmentioning

confidence: 99%

Deciphering the Antibacterial Mechanisms of 5-Fluorouracil in Escherichia coli through Biochemical and Transcriptomic Analyses

Zhang,

Song,

Yang

et al. 2024

Antibiotics

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“…However, there are also strains that are opportunistic or highly pathogenic to humans when consumed through food (e.g., strains belonging to serotype O157:H7, etc.) [ 3 , 4 ]. S. aureus is widely found in the environment and secretes virulence factors that can easily contaminate meat, egg products and milk [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

Synthesizing Carbon Quantum Dots via Hydrothermal Reaction to Produce Efficient Antibacterial and Antibiofilm Nanomaterials

Cui,

Fan,

Liu

et al. 2023

Foods

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This study aimed to synthesize antibacterial carbon quantum dots (SP-CDs) from polyethyleneimine and spermidine via hydrothermal reaction. It was revealed that SP-CDs, with small size (7.18 nm) and high positive charge (+31.15 mV), had good fluorescence properties and lots of amino groups on their surfaces. The inhibition effect of SP-CDs on Staphylococcus aureus was better than that towards Escherichia coli, and the SP-CDs also had an inhibitory effect on multi-drug-resistant E. coli. The mechanism of SP-CDs shows that the SP-CDs were adsorbed on the surface of the negatively charged cell membrane through electrostatic interaction. SP-CDs can cause changes in membrane permeability, resulting in a shift of the cell membrane from order to disorder and the decomposition of chemical components, followed by the leakage of cell contents, resulting in bacterial death. SP-CDs can also significantly inhibit biofilm formation, destroy mature biofilms and reduce the number of living cells. Moreover, SP-CDs had negligible antimicrobial resistance even after 18 generations of treatment. This study proves that SP-CDs effectively inhibit the proliferation of foodborne pathogens, providing new feasibility for the application of carbon-based nanomaterials in the food industry.

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Terminalia catappa leaf extract as a bio-reducing agent to synthesize Cu2O nanoparticles for methylene blue photodegradation

Nguyen,

Nguyen

et al. 2024

Discov Appl Sci

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The large tree species Terminalia catappa is a member of the Combretaceae family and is mainly found in tropical climates. They are commonly cultivated for shade because they have huge, dense foliage. Numerous polyphenols, including flavonoids, tannins, saponins, and phytosterols, are present in the leaves. In this study, the green chemical method was used to extract polyphenols from dried green almond leaves. They were employed in the synthesis of Cu2O nanoparticles as a reducing agent. FTIR and UV–Vis were used to describe the leaf extract of Terminalia catappa after the chlorophyll was removed. Copper salt was used to create Cu2O nanoparticles via a reduction process. The extract's potential for photocatalytic dye degradation has also been explored. The obtained Cu2O had a spherical shape with dimensions of 50–100 nm, and its band gap energy reached 1.945 eV to remove methylene blue from aqueous media under visible light irradiation conditions. At an initial MB concentration of 10 ppm, the decomposition efficiency reached 71.99% after only 2 h of exposure to simulated sunlight. The decomposition process occurred according to a pseudo-first-order kinetic model with a rate constant of 0.0084 min−1.

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Antibacterial mechanism of kojic acid and tea polyphenols against Escherichia coli O157:H7 through transcriptomic analysis

Cited by 6 publications

References 70 publications

Deciphering the Antibacterial Mechanisms of 5-Fluorouracil in Escherichia coli through Biochemical and Transcriptomic Analyses

Deciphering the Antibacterial Mechanisms of 5-Fluorouracil in Escherichia coli through Biochemical and Transcriptomic Analyses

Synthesizing Carbon Quantum Dots via Hydrothermal Reaction to Produce Efficient Antibacterial and Antibiofilm Nanomaterials

Terminalia catappa leaf extract as a bio-reducing agent to synthesize Cu2O nanoparticles for methylene blue photodegradation

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