Antibacterial mechanisms of Zataria multiflora Boiss. essential oil against Lactobacillus curvatus

Ziaee, Esmaeil; Razmjooei, Maryam; Shad, Ehsan; Eskandari, Mohammad Hadi

doi:10.1016/j.lwt.2017.08.089

Cited by 49 publications

(33 citation statements)

References 35 publications

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“…and clove buds (Syringa yunnanensis), on Salmonella dysenteriae and S. aureus (Xu et al 2016;Guo et al 2017). Regarding the effect of EOs on membrane integrity of beneficial bacteria, a study conducted by (Ziaee et al 2018) has shown that Z. multiflora EO affected the membrane integrity of L. curvatus as confirmed by the high losses of nucleic acids and proteins. In our study, the highest concentration of BOT produced only 2Á4 times more release of proteins from L. rhamnosus cells.…”

Section: Discussionmentioning

confidence: 99%

In vitromechanism of antibacterial action of a citrus essential oil on an enterotoxigenicEscherichia coliandLactobacillus rhamnosus

2020

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Aim: This study investigated the in vitro mechanism of action of a commercial citrus EO, Brazilian orange terpenes (BOT), on an enterotoxigenic Escherichia coli (ETEC) isolated from pig gut and on Lactobacillus rhamnosus. Methods and Results: Firstly, bacteria were exposed sequentially to BOT every 3 h (three times) at sub-minimal inhibitory concentrations and results showed that sequential exposure to BOT provoked a higher reduction of bacteria viability than a single exposure and the reduction of ETEC viability was higher compared to that of L. rhamnosus. Then, evaluation of the BOT effects on the cell membrane permeability and integrity, indicated that BOT increased the membrane permeability and caused disruptive effects on the integrity of bacterial cells as reflected by an increase of the relative electric conductivity and the release of essential cell constituents. Interestingly, BOT effects were more pronounced on the ETEC than on L. rhamnosus. This was ratified by scanning electron microscopy, which showed more noticeable morphological damages and disturbances on ETEC cells than on the L. rhamnosus cells. Limonene was detected as the major compound in BOT by polar/nonpolar GC-MS (78Á65%/79Á38%). Conclusions: Results revealed that the probable mechanism of the selective antibacterial action of the citrus EO, BOT, can be described as altering more remarkable the permeability and integrity of the cytoplasmic membrane as well as the external structure of ETEC cells than L. rhamnosus cells. Significance and Impact of the Study: This study provides information about the mechanism of antibacterial action displayed by a citrus EO, a by-product of the citrus processing industry, as a natural alternative to antibiotics used in pig production sector to combat pathogens such as ETECs.

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Section: Discussionmentioning

confidence: 99%

In vitromechanism of antibacterial action of a citrus essential oil on an enterotoxigenicEscherichia coliandLactobacillus rhamnosus

2020

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“…Volatile organic compounds (VOCs) are low molecular weight compounds that can vaporize and enter the gas phase at normal atmospheric temperatures and pressure . The extraction of volatile and aromatic essential oils from plants has been the main source of industrial production of fragrances, cosmetics, mosquito repellents, and other products . However, due to the lack of land resources and the increasing cost of manpower and machinery, the protection of the environment these seem to have become difficult .…”

Section: Introductionmentioning

confidence: 99%

“…and other products [8,9]. However, due to the lack of land resources and the increasing cost of manpower and machinery, the protection of the environment these seem to have become difficult [10].…”

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confidence: 99%

Changes of volatile organic compounds and bioactivity of Alternaria brassicae GL07 in different ages

et al. 2019

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Plant endophytes are rich in secondary metabolites and are widely used in medicine, chemical, food, agriculture, and other fields. Here, an endophytic fungus is isolated from Ginkgo biloba L. leaves and identified as Alternaria brassicae GL07 through genotypic characterizations. It can produce fruity scented volatiles. The analysis of volatile organic compounds (VOCs) was done by gas chromatography–mass spectrometry. A total of 32 components were identified; and at different culture times, the composition of VOCs was different. It had more components in the first two weeks, but a fewer components on the 21st day. More olefins, ketone, aldehyde, and alcohol were found in the growth period and more amines and esters were found in the decline period. Also, 2,5‐dihydroxyacetophenone, β‐ionone, and nonanal were found. They were the same ingredients in Ginkgo essential oils and some of them were isolated for the first time in the volatile constituents of endophytes. The antioxidant activity and whitening activities of all extracts were also evaluated. When cultured for 10 days, it had the strongest 2,2‐diphenyl‐2‐picrylhydrazyl radical (IC50, 0.56 g/L), hydroxyl radical scavenging ability (IC50, 0.47 g/L), reducing ability, and tyrosinase inhibition ability (IC50, 5.18 g/L), which may be due to a large amount of ketones and alcohols produced during the log phase. This demonstrates the potential of A. brassicae GL07 to produce bioactive compounds and to be used for perfume and cosmetic industries.

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“…It has been shown that herbal medicines, especially ZMB and its components, do not have any harmful effects on humans. The availability and pharmacokinetics data of carvacrol and thymol showed that there are no acute and short‐term in vivo effects on human and animal health . It is important to evaluate other components of the ZMB that have antimicrobial effects or could improve immune system functions.…”

Section: Discussionmentioning

confidence: 99%

“…The availability and pharmacokinetics data of carvacrol and thymol showed that there are no acute and short-term in vivo effects on human and animal health. 37,38 It is important to evaluate other components of the ZMB that have antimicrobial effects or could improve immune system functions. These materials could be used individually or in combination of other active ingredient of other plants or with special new antibiotic to overcome the resistant isolate of A. baumannii or other bacteria.…”

Section: Discussionmentioning

confidence: 99%

In vivo antibacterial activity of Zataria multiflora Boiss extract and its components, carvacrol, and thymol, against colistin‐resistant Acinetobacter baumannii in a pneumonic BALB/c mouse model

Hassannejad

Bahador

Rudbari

et al. 2019

J of Cellular Biochemistry

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Background Acinetobacter baumannii has emerged as a major cause of nosocomial infections. Various resistance mechanisms of A. baumannii against antibiotics have transformed it into a successful nosocomial pathogen. Because of the limited number of available antibiotics, we used a medicinal plant with an antibacterial effect. Zataria multiflora Boiss (ZMB) extract and its components were used for the treatment of pneumonic mice infected with A. baumannii. The biological effects of this extract and the regulation of the outer membrane protein A (ompA) gene were used in a mouse model. Methods A pneumonic mouse model was prepared using clinical and standard strains (1.5 × 108 colony‐forming units/mL) of A. baumannii. BALB/c mice groups were treated with a ZMB extract, carvacrol, thymol, and sensitive antibiotics. The lung tissues of the treated mice were cultured for 5 days and each day, bacterial clearance and the ompA gene expression were assessed by quantitative real‐time polymerase chain reaction. Results In the lung tissue culture of pneumonic mice infected with standard or clinical isolate, no colony was detected when treated with the ZMB extract after 2 and 3 days (P < 0.01), respectively. In the carvacrol‐treated group, bacterial clearance was seen at day 4 and day 5 (P < 0.05). Bacterial clearance was seen 5 days after treatment with thymol and imipenem and 6 days after ampicillin/sulbactam treatment. The regulation of ompA gene was significantly decreased in this order: ZMB extract, carvacrol, thymol, imipenem, and ampicillin/sulbactam. Discussion The ZMB extract had a potent bactericidal effect against A. baumannii that could downregulate the ompA gene. ZBM extract and carvacrol could be novel therapeutic agents for antibiotic‐resistant A. baumannii.

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Antibacterial mechanisms of Zataria multiflora Boiss. essential oil against Lactobacillus curvatus

Cited by 49 publications

References 35 publications

In vitromechanism of antibacterial action of a citrus essential oil on an enterotoxigenicEscherichia coliandLactobacillus rhamnosus

In vitromechanism of antibacterial action of a citrus essential oil on an enterotoxigenicEscherichia coliandLactobacillus rhamnosus

Changes of volatile organic compounds and bioactivity of Alternaria brassicae GL07 in different ages

In vivo antibacterial activity of Zataria multiflora Boiss extract and its components, carvacrol, and thymol, against colistin‐resistant Acinetobacter baumannii in a pneumonic BALB/c mouse model

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