Antibacterial pathway of cefquinome against Staphylococcus aureus based on label-free quantitative proteomics analysis

Gao, Linglin; Zhu, Huanzhang; Chen, Yun; Yang, Yuhui

doi:10.1007/s12275-021-1201-x

Cited by 1 publication

(1 citation statement)

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“…The cell membrane plays an important role in the metabolism and proliferation of bacteria [43,44], and previous studies found that bacteria was killed by cefquinome through damaging the cell membrane [45]. As a result of that, we hypothesized that there was a bactericidal effect by enhancing the cell membrane destruction effect of cefquinome when we combined garlic oil and cefquinome, which caused metabolic and replication disorders.…”

Section: Discussionmentioning

confidence: 92%

Detection of Antibiotic Resistance in Feline-Origin ESBL Escherichia coli from Different Areas of China and the Resistance Elimination of Garlic Oil to Cefquinome on ESBL E. coli

Tong

Yang

et al. 2023

IJMS

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The development of drug-resistance in the opportunistic pathogen Escherichia coli has become a global public health concern. Due to the share of similar flora between pets and their owners, the detection of pet-origin antibiotic-resistant E. coli is necessary. This study aimed to detect the prevalence of feline-origin ESBL E. coli in China and to explore the resistance elimination effect of garlic oil to cefquinome on ESBL E. coli. Cat fecal samples were collected from animal hospitals. The E. coli isolates were separated and purified by indicator media and polymerase chain reaction (PCR). ESBL genes were detected by PCR and Sanger sequencing. The MICs were determined. The synergistic effect of garlic oil and cefquinome against ESBL E. coli was investigated by checkerboard assays, time-kill and growth curves, drug-resistance curves, PI and NPN staining, and a scanning electronic microscope. A total of 80 E. coli strains were isolated from 101 fecal samples. The rate of ESBL E. coli was 52.5% (42/80). The prevailing ESBL genotypes in China were CTX-M-1, CTX-M-14, and TEM-116. In ESBL E. coli, garlic oil increased the susceptibility to cefquinome with FICIs from 0.2 to 0.7 and enhanced the killing effect of cefquinome with membrane destruction. Resistance to cefquinome decreased with treatment of garlic oil after 15 generations. Our study indicates that ESBL E. coli has been detected in cats kept as pets. The sensitivity of ESBL E. coli to cefquinome was enhanced by garlic oil, indicating that garlic oil may be a potential antibiotic enhancer.

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Section: Discussionmentioning

confidence: 92%