Antibacterial peptides from Monochamus alternatus induced oxidative stress and reproductive defects in pine wood nematode through the ERK/MAPK signaling pathway

Yu, Lupeng; Yang, Meijiao; Jiang, Di; Jin, Huanhuan; Jin, Zhongwu; Chu, Xiangxiang; Zhao, Ming-Jie; Wu, Songqing; Zhang, Feiping; Hu, Xia

doi:10.1016/j.pestbp.2023.105511

Cited by 4 publications

(3 citation statements)

References 65 publications

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“…22,23 Currently, research in this area predominantly involves analyzing the JPS transcriptome without a reference genome, along with gene function validation using RNA interference (RNAi) and other methods. 24,25 However, due to the lack of reference genome, high-throughput sequencing does not provide comprehensive information. 26 Additionally, most known proteins that determine the PCA of JPS were obtained through homology analyses of related functional proteins reported in other species, including Muc91C 12 and Toll6/Toll8.…”

Section: Introductionmentioning

confidence: 99%

“…Understanding the molecular mechanisms that determine the PCA of JPS is vital for elucidating PWN‐JPS assembly 22,23 . Currently, research in this area predominantly involves analyzing the JPS transcriptome without a reference genome, along with gene function validation using RNA interference (RNAi) and other methods 24,25 . However, due to the lack of reference genome, high‐throughput sequencing does not provide comprehensive information 26 .…”

Section: Introductionmentioning

confidence: 99%

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Identification of pine wood nematode (Bursaphelenchus xylophilus) loading response genes in Japanese pine sawyer (Monochamus alternatus) through comparative genomics and transcriptomics

Zou,

Jia,

Zhu

et al. 2024

Pest Management Science

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BACKGROUNDPine wood nematode (PWN; Bursaphelenchus xylophilus) is the causative agent of pine wilt disease (PWD), which is considered the most dangerous biohazard to conifer trees globally. The transmission of PWN relies on insect vectors, particularly the Japanese pine sawyer (JPS; Monochamus alternatus). However, the molecular mechanism underlying PWN‐JPS assembly remains largely unknown.RESULTSHere, we found that both geographical and gender could significantly affect the PCA (PWN carrying amount) of JPS; thus, JPS transcriptomes from diverse locations and genders were explored regard to PWN loading. Due to the shortage of genomes, we developed a full‐length reference transcriptome for analyzing next‐generation sequencing data. A comparative genomic study was performed, and 11 248 potential PWN‐carrying associate genes (β) were nominated in JPS by using the reported genomes of PWN and non‐PWN carrier insect species. Then, 151 differentially expressed transcripts (DETs), 28 of them overlapped with β, correlated with the PCA of JPS were nominated by RNA‐Seq, and found that fatty acid β‐oxidation might be the key factor that affected the PCA of JPS. Furthermore, JPS fatty acid β‐oxidation rates were experimentally decreased using the inhibitor Etomoxir, leading to an increased PCA of JPS. Meanwhile, silencing MaCPT1 in JPS by RNA interference led to a decreased fatty acid β‐oxidation rate and increased PCA of JPS.CONCLUSIONSIn conclusion, MaCPT1 was able to decrease the PWN‐JPS assembly formation through the fatty acid β‐oxidation of JPS. These results provide new insights for exploring the impact of PWN invasion on JPS.This article is protected by copyright. All rights reserved.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Identification of pine wood nematode (Bursaphelenchus xylophilus) loading response genes in Japanese pine sawyer (Monochamus alternatus) through comparative genomics and transcriptomics

Zou,

Jia,

Zhu

et al. 2024

Pest Management Science

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“…In Helicoverpa armigera , reactive oxygen species (ROS) mediates MEK/ERK signaling and acts as an important regulator of diapause maintenance (Lin et al, 2021). Recently, it also revealed that the antibacterial peptide S‐defensin induced oxidative stress and reproductive defects in pine wood nematode via the ERK/MAPK signaling pathway (Yu et al, 2023).…”

Section: Introductionmentioning

confidence: 99%

Identification and characteristic analysis of an extracellular signal‐regulated kinase from Ostrinia furnacalis Guenée

Li,

Xu,

Zhang

et al. 2023

Arch Insect Biochem Physiol

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The extracellular signal‐regulated kinase (ERK) pathway, a critical genetic determinant, controls diverse physiological functions, including innate immunity, development, and stress response. In the current study, a full‐length cDNA (1592bp) encoding the ERK gene (OfERK) was cloned from Ostrinia furnacalis Guenée (GenBank accession number: MF797866). The open reading frame of the OfERK gene encoded 364 amino acids and shared 96.43%–98.08% amino acid identities with other insect mitogen‐activated protein kinases. For spatiotemporal analysis of the expression pattern, OfERK exhibited a significant peak expression on the 3rd day of the pupa stage and showed the highest expression in hemocytes specifically. Indirect immunofluorescence assays and immuno‐electron microscopy revealed a wide distribution of the OfERK protein in hemocytes and epidermis. Moreover, the results demonstrated that the Bt Cry1Ab‐activated toxin significantly induces the expression of OfERK. Other genes related to immune response, development, and stress response exhibited dynamic changes in expression after Cry1Ab oral treatment. The expression of OfERK was downregulated through RNA interference, and the correlation of its expression with other related genes was verified using quantitative real‐time polymerase chain reaction. Our study provides valuable insights into the regulatory mechanism of ERK in insects for future studies.

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Discovery of novel nematicidal active ingredient 2,4,7-trichloroquinazoline as a potential succinic dehydrogenase inhibitor

Wang,

Du,

Song

et al. 2024

Physiological and Molecular Plant Pathology

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Antibacterial peptides from Monochamus alternatus induced oxidative stress and reproductive defects in pine wood nematode through the ERK/MAPK signaling pathway

Cited by 4 publications

References 65 publications

Identification of pine wood nematode (Bursaphelenchus xylophilus) loading response genes in Japanese pine sawyer (Monochamus alternatus) through comparative genomics and transcriptomics

Identification of pine wood nematode (Bursaphelenchus xylophilus) loading response genes in Japanese pine sawyer (Monochamus alternatus) through comparative genomics and transcriptomics

Identification and characteristic analysis of an extracellular signal‐regulated kinase from Ostrinia furnacalis Guenée

Discovery of novel nematicidal active ingredient 2,4,7-trichloroquinazoline as a potential succinic dehydrogenase inhibitor

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