Antibacterial photodynamic therapy with curcumin and Curcuma xanthorrhiza extract against Streptococcus mutans

Lee, Hyung Jung; Kang, Si Mook; Jeong, Seung Hwa; Chung, Ki Ho; Kim, Baek Il

doi:10.1016/j.pdpdt.2017.09.003

Cited by 80 publications

(64 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In addition, hydrophobic molecules are prone to aggregation in physical media, disturbing membrane binding and ROS generation [30,31]. In our previous study, higher concentration (10 4 ng/mL) of curcumin has been shown to be ineffective in aPDT against S. mutans compared to lower concentration [32]. This observation could be due to aggregation of curcumin and its inability to dissolve sufficiently at high concentration.…”

Section: Discussionmentioning

confidence: 99%

Susceptibility of oral bacteria to antibacterial photodynamic therapy

Kang

Jung

Kim

2019

Journal of Oral Microbiology

Self Cite

View full text Add to dashboard Cite

Effective methods for managing the oral microbiome are necessary to ensure not only the oral but also the systemic health of a human body. The purpose of this study was to determine the sensitivity of four photosensitizers (PSs) to blue light in six representative oral bacterial species that cause intraoral diseases. The following six strains were investigated: Actinomyces israelii , Enterococcus faecium , Fusobacterium nucleatum , Lactobacillus gasseri , Streptococcus mutans, Veillonella parvula . PS stock solutions (1 mg/ml) were prepared by dissolving curcumin and protoporphyrin-IX in dimethyl sulfoxide, and resazurin and riboflavin in distilled water. The inoculation of 20 ml of a bacterial suspension cultured for 24 hours was mixed with 1,980 ml of each test solution, and then a light source was placed in front of the mixture. The irradiation wavelength was 405 nm and its applied energy was 25.3 J. The independent-samples t -test and one-way analysis of variance within groups were performed to compare the antibacterial effects in the four PSs. The antibacterial susceptibility when using different PSs and visible blue-light irradiation differed between the bacterial strains. Antibacterial photodynamic therapy that includes light exposure and PSs can be used to control the oral bacteria strains related to oral disease.

show abstract

Section: Discussionmentioning

confidence: 99%

Susceptibility of oral bacteria to antibacterial photodynamic therapy

Kang

Jung

Kim

2019

Journal of Oral Microbiology

Self Cite

View full text Add to dashboard Cite

show abstract

“…Furthermore, the enhanced antitumour activity was noted after UVB irradiation of curcumin by caspase activation on HaCaT (human keratinocyte cell) cells. It was also efficient against MCF-7 breast cancer cells at 30 J/cm 2 [ 77 , 78 ]. These data suggested that curcumin may act as a potent anticancer agent by preventing cancer progression, migration and invasion [ 129 , 130 , 131 ].…”

Section: Natural Photoactive Compounds From Plantsmentioning

confidence: 99%

Role of Photoactive Phytocompounds in Photodynamic Therapy of Cancer

et al. 2020

View full text Add to dashboard Cite

Cancer is one of the greatest life-threatening diseases conventionally treated using chemo- and radio-therapy. Photodynamic therapy (PDT) is a promising approach to eradicate different types of cancers. PDT requires the administration of photosensitisers (PSs) and photoactivation using a specific wavelength of light in the presence of molecular oxygen. This photoactivation exerts an anticancer effect via apoptosis, necrosis, and autophagy of cancer cells. Recently, various natural compounds that exhibit photosensitising potentials have been identified. Photoactive substances derived from medicinal plants have been found to be safe in comparison with synthetic compounds. Many articles have focused on PDT mechanisms and types of PSs, but limited attention has been paid to the phototoxic activities of phytocompounds. The reduced toxicity and side effects of natural compounds inspire the researchers to identify and use plant extracts or phytocompounds as a potent natural PS candidate for PDT. This review focusses on the importance of common photoactive groups (furanocoumarins, polyacetylenes, thiophenes, curcumins, alkaloids, and anthraquinones), their phototoxic effects, anticancer activity and use as a potent PS for an effective PDT outcome in the treatment of various cancers.

show abstract

“… In our previous study, we showed that when curcumin was combined with visible blue light, it could eradicate the skin‐disease‐causing microbe Propionibacterium acnes by a series of cytotoxic actions . There is also a report showing the application of curcumin in antibacterial PDT against the dental‐caries‐related microbe Streptococcus mutans . Despite these useful discoveries, the detailed mechanisms and which proteins are influenced to achieve the bactericidal effect of curcumin are still unclear.…”

Section: Introductionmentioning

confidence: 99%

“…17 There is also a report showing the application of curcumin in antibacterial PDT against the dental-caries-related microbe Streptococcus mutans. 18 Despite these useful discoveries, the detailed mechanisms and which proteins are influenced to achieve the bactericidal effect of curcumin are still unclear. To date, it has only been revealed that the photo-cytotoxicity of curcumin comes from a decrease in intracellular glutathione and mediated ROS generation.…”

mentioning

confidence: 99%

Identification of carbonylated proteins in a bactericidal process induced by curcumin with blue light irradiation on imipenem‐resistant Acinetobacter baumannii

Chang

Cheng

Lai

et al. 2019

Rapid Comm Mass Spectrometry

View full text Add to dashboard Cite

Rationale Antimicrobial photodynamic treatment is potentially an alternative to antibiotics and is also effective against viruses, fungi and some cancers. Our previous studies have shown that blue light combined with curcumin, a chemical from the turmeric plant, exerted effective antimicrobial activity via photodynamic treatment. The study reported in this paper investigates which target proteins are affected after the treatment. Methods We treated imipenem‐resistant Acinetobacter baumannii with blue light and curcumin and used protein carbonylation as a marker for oxidative damage. After treatment, the bacterial proteins were extracted and the protein carbonyls marked using dinitrophenylhydrazide. After enzyme digestion, we used liquid chromatography/nano‐electrospray ionization (LC/nano‐ESI) ion trap mass spectrometry to identify bacterial peptides from a customized database. The functional enrichment analyses of the identified proteins were performed using gene ontology annotation and the STRING protein–protein interaction network. Results The application of curcumin with blue light showed good antibacterial activity against imipenem‐resistant A. baumannii. Using a shotgun proteomics approach, the carbonylated proteins in A. baumannii caused by the photolytic curcumin were identified. The results showed that the proteins related to membrane structures, translation and response to oxidative stress were preferentially modified. Conclusions The photolytic curcumin treatment could be a potential alternative to antibiotics for bacterial infection. In this study, the shotgun proteomics strategy allows us to explore the possible bactericidal mechanisms under this oxidative stress. The result provides a reference for future studies on the enhancement of the action of photolytic curcumin.

show abstract

Antibacterial photodynamic therapy with curcumin and Curcuma xanthorrhiza extract against Streptococcus mutans

Cited by 80 publications

References 28 publications

Susceptibility of oral bacteria to antibacterial photodynamic therapy

Susceptibility of oral bacteria to antibacterial photodynamic therapy

Role of Photoactive Phytocompounds in Photodynamic Therapy of Cancer

Identification of carbonylated proteins in a bactericidal process induced by curcumin with blue light irradiation on imipenem‐resistant Acinetobacter baumannii

Contact Info

Product

Resources

About