Antibacterial Potential of Aqueous Neem Leaf Extract (Azadirachta indica A. Juss) on Spermatozoa Quality in Extended Porcine Semen

Abstract

Aim:The present study was investigated to assess the antibacterial potential of aqueous neem leaves extracts (ANLE) on spermatozoa quality in extended porcine semen. Materials and Methods: Fresh semen was collected from a mature and intact boar (age, breed, body condition score, health status) using the glove-hand technique. The collected semen samples were diluted and allotted to six treatments with three replicates per treatment in a completely randomized design and evaluated at 0, 24 and 48 h of refrigeration at 17°C. Semen quality parameters such as progressive motility (%), viability (%), morphology (%), pH, acrosome integrity (%), and bacteria load (×10 4 CFU/mL) were evaluated. Results and Discussion: At 48 h, a significant difference (p < 0.05) in motility was observed across the treatments with Treatment 1 giving the highest mean value (84.67±2.40) and Treatment