Antibacterial potential of essential oils against planktonic and sessile cells of Escherichia coli isolated from diarrhea cases in swine

Abstract

Escherichia coli is a pathogen associated with infections in piglets in the post-weaning phase, its pathogenicity is related to the animal's susceptibility to bacterial enterotoxins. The objective of the present study was to determine the EOs activity against E. coli strain, in the form planktonic and sessile. Although the Disc-Diffusion tests to determine the Minimum Inhibitory Concentration, do not fully corroborate with the other analyzes of this study, it was noticed bacteria inhibition. The EOs were prepared at 0.4%, 0.8% and 1.0% for tests. The tested EOs were effective against E. coli planktonic cells (p<0.05). As for the sessile cells, the most significant result was inhibition and 100% sessile cells at the concentration of 1.0% of Cymbopogon citratus EO. Although there was resistance in some treatments, the tested EOs demonstrated inhibition capacity, constituting promising alternatives for the control of E. coli, especially of planktonic cells.

Antibacterial activity of the essential oil of Thymus vulgaris and thymol in the control of Curtobacterium flaccumfaciens

“…There is a lack of studies in the literature reporting the activity of plant metabolites as antimicrobial against cff in the plankton and biofilm form, although many studies have been published reporting the antibacterial effect of these substances (Bellaver et al, 2022;Lima et al, 2017Nostro et al, 2007, Valeriano et al, 2012. In the present study, the results of the antibiofilm tests demonstrated that the EO of thyme and thymol are promising against cff.…”

“…The chemical characterization of Thymus vulgaris essential oil was performed by Bellaver et al (2022), containing its chemical composition identified as Table 1. For the evaluation of the antibiotic effect of the EO of thyme and thymol, 100 µL of standardized inoculum and 100 µL of EO/thymol-based solution were added in sterile 96-well microplates.…”

“…The formation of biofilms causes phenotypic changes of planktonic cells, which can be described as strategies for survival of microorganisms in adverse conditions Brugnera;Piccoli, 2010). Studies show that cells organized in biofilms become more resistant to the effects of antimicrobials, when compared to planktonic cells of the same strain (Costa et al, 2014;Bellaver et al, 2022).…”

Antimicrobial and Cytotoxic Bioprospection of Thymus vulgaris and Thymol Against Salmonella enterica Serovar Heidelberg Isolated in Broiler Chicken

“…There are many studies on the antibacterial activity of EOs [9][10][11][12], especially with TEO [16][17][18] and thymol [23,24]. However, research on the activity of the compounds used in this work against SH is scarce.…”

“…The safe and sustainable production of the meat, free of Salmonella, opens the way to new markets. Several studies in the literature report the antibacterial activity of essential oils (EOs) and their isolated compounds [9][10][11][12]. These are substances derived from secondary plant metabolism, such as defense mechanisms of plants against pests and pathogens [13,14].…”