Antibacterial Potential of <i>Aloe weloensis</i> (Aloeacea) Leaf Latex against Gram-Positive and Gram-Negative Bacteria Strains

Emiru, Yohannes Kelifa; Siraj, Ebrahim Abdela; Teklehaimanot, Tekleab Teka; Amare, Gedefaw Getnet

doi:10.1155/2019/5328238

Cited by 15 publications

(12 citation statements)

References 23 publications

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“…The sap was, then, left in open air for three days to allow evaporation of water, which yielded a pale yellow latex. Finally, after drying, the dried latex was transferred into a vial and, then, kept in a refrigerator until further use [ 13 – 15 ].…”

Section: Methodsmentioning

confidence: 99%

“…weloensis (Eret tafa) is traditionally used for the treatment of malaria, wound, different skin diseases and pain from ear infection, headache, and rheumatism. An ethnobotanical survey conducted in Gubalafto, Northeast Ethiopia, reported that the leaf latex of the plant is taken orally to treat malaria [ 12 , 13 ]. However; the antimalarial activity of this medicinal plant is not scientifically studied.…”

Section: Introductionmentioning

confidence: 99%

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Antimalarial Activity of the Leaf Latex ofAloe weloensis(Aloaceae) againstPlasmodium bergheiin Mice

Awgichew

Kassahun

2020

Journal of Tropical Medicine

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Background. Emergence of drug resistance and lack of therapeutic efficacy of modern antimalarial drugs are the most triggering factors for the searching of new lead compounds with different mechanisms of action. Medicinal plants with documented traditional uses are a viable option for treatment of malaria. Traditionally, the leaf latex of Aloe weloensis has been used in the treatment of malaria in Ethiopia. Hence, this study was undertaken to investigate the antimalarial activity of the leaf latex of Aloe weloensis in Plasmodium berghei-infected mice. Methods. A four-day suppressive test was employed to evaluate the antimalarial effect of the leaf latex of the plant against P. berghei in Swiss albino mice. Mice were randomly assigned in five groups of five animals in each and given 100, 200, and 400 mg/kg of the leaf latex, chloroquine 25 mg/kg, and distilled water. The level of parasitemia, packed cell volume, survival time, temperature, and body weight was used to determine the antimalarial activity. Results. The acute toxicity study indicated that the leaf latex of A. weloensis caused neither mortality nor signs and symptoms of toxicity at a dose of 2000 mg/kg. Furthermore, the 4-day suppressive test indicated that the latex of the plant exhibited a significant parasitemia reduction in a dose-dependent manner as compared to negative control. The leaf latex of the plant exhibited a percent inhibition of 13.05%, 41.87%, and 66.84% at doses of 100 mg/kg, 200 mg/kg, and 400 mg/kg, respectively. The chemosuppression of the antimalarial activity was statistically significant at 100 mg/kg (p<0.05), 200 mg/kg (p<0.01), and 400 mg/kg (p<0.01) as compared to negative control. All doses of the leaf latex prevented weight loss and reduction in temperature and packed cell volume and increased the survival time of infected mice. Conclusion. The results of this study demonstrated that the leaf latex of Aloe weloensis possessed antiplasmodial activity confirming the genuine traditional use of the plant as an antimalarial agent.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Antimalarial Activity of the Leaf Latex ofAloe weloensis(Aloaceae) againstPlasmodium bergheiin Mice

Awgichew

Kassahun

2020

Journal of Tropical Medicine

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“…Between 1981 and 2014, 43.6% of anti-infective drugs were approved and 40.7% of anticancer agents were based on natural products or derivatives thereof [ 22 ], and many natural products have become a template for drug design because they often present a ligand–protein binding motif [ 23 ]. Some natural products have shown antibacterial activity like as described by Nofiani et al [ 24 ] and Emiru et al [ 25 ].…”

Section: Introductionmentioning

confidence: 99%

Searching Hit Potential Antimicrobials in Natural Compounds Space against Biofilm Formation

2020

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Biofilms are communities of microorganisms that can colonize biotic and abiotic surfaces and thus play a significant role in the persistence of bacterial infection and resistance to antimicrobial. About 65% and 80% of microbial and chronic infections are associated with biofilm formation, respectively. The increase in infections by multi-resistant bacteria instigates the need for the discovery of novel natural-based drugs that act as inhibitory molecules. The inhibition of diguanylate cyclases (DGCs), the enzyme implicated in the synthesis of the second messenger, cyclic diguanylate (c-di-GMP), involved in the biofilm formation, represents a potential approach for preventing the biofilm development. It has been extensively studied using PleD protein as a model of DGC for in silico studies as virtual screening and as a model for in vitro studies in biofilms formation. This study aimed to search for natural products capable of inhibiting the Caulobacter crescentus enzyme PleD. For this purpose, 224,205 molecules from the natural products ZINC15 database, have been evaluated through molecular docking and molecular dynamic simulation. Our results suggest trans-Aconitic acid (TAA) as a possible starting point for hit-to-lead methodologies to obtain new inhibitors of the PleD protein and hence blocking the biofilm formation.

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“…They have been demonstrated antibacterial, antitumor, antiin ammatory, anti-arthritic, anti-rheumatoid, anticancer and antidiabetic activities (16). The latex of Aloe weloensis leaf showed antibacterial effect against gram negative and gram positive strains (17). The leaf latex of the plant has been used in folk medicine against malarial and others human ailment in Ethiopia (18).The leaf latex and isolated compounds of Aloe vera, Aloe pulcherrima and Aloe megalacantha, a similar plant species, possessed signi cant antimalarial activity (19)(20)(21).As a result, Aloe weloensis may have similar effect as all plants belong to the same genus.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of in vitro and in vivo Antiplasmodial Activity of the Leaf Latex of Aloe Weloensis (Aloaceae)

Amare

Awgichew

Ahmed

et al. 2020

Preprint

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Background: Nature has gifted a variety of plants having potential effect against plasmodium parasites. The present study was aimed to determine in vitro and in vivo antimalarial activity of the leaf latex of Aloe weloensis.Methods: In vitro antimalarial activity of the leaf latex of A. weloensis was determined against 3D7 strain of P. falciparum. Antimalarial activity of the three doses the latex was evaluated in 4 day-suppressive and curative models against P. berghei infected mice. Antioxidant activity of the leaf latex of A. weloensis was assessed in 2,2- diphenyl 1- picrylhydrazine assay model. Results: Antioxidant activity of the latex was concentration dependent; the strongest inhibition was measured at 400 μg/mL (73.54%). The leaf latex of A. weloensis was demonstrated inhibitory activity against 3D7 malarial strain (IC50 = 9.14 μg/ml). Suppressive and curative effect of the latex was found to be dose dependent. Parasitemia reduction was significant (200 mg/kg, p<0.01, 400 and ,600 mg/kg, p<0.001) in 4-day suppressive test compared to vehicle control. Parasitemia level of the mice treated with 200, 400 and 600 mg/kg doses of the latex significantly (p<0.001) reduced with suppression of 36%, 58% and 64% respectively in curative test. Administration of the leaf latex of A. weloensis significantly (p<0.01) improved mean survival time, pack cell volume, rectal temperature and body weight of P. berghei infected mice. Conclusion: The finding showed that the leaf latex of Aloe weloensis endowed prominent antimalarial and antioxidant activities. The result can serve as a step towards the development of safe and effective herbal therapy against plasmodium parasites.

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Antibacterial Potential of Aloe weloensis (Aloeacea) Leaf Latex against Gram-Positive and Gram-Negative Bacteria Strains

Cited by 15 publications

References 23 publications

Antimalarial Activity of the Leaf Latex ofAloe weloensis(Aloaceae) againstPlasmodium bergheiin Mice

Antimalarial Activity of the Leaf Latex ofAloe weloensis(Aloaceae) againstPlasmodium bergheiin Mice

Searching Hit Potential Antimicrobials in Natural Compounds Space against Biofilm Formation

Evaluation of in vitro and in vivo Antiplasmodial Activity of the Leaf Latex of Aloe Weloensis (Aloaceae)

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