ANTIBACTERIAL POTENTIAL OF KAPUL FRUIT SKIN (Baccaurea macrocarpa) ON Streptococcus sanguis

Norhayati, Norhayati; Ujrumiah, Siti; Noviany, Annisa; Carabelly, Amy Nindia

doi:10.30659/odj.6.2.118-124

Cited by 5 publications

(5 citation statements)

References 2 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…S. sanguinis memiliki peran dalam rongga mulut manusia sebagai bakteri pionir proses kolonisasi bakteri. 3 S. sanguinis merupakan jenis golongan bakteri Streptococcus viridans (S. viridans) dan termasuk pada tipe alfa hemolitik. S. sanguinis dengan bentuk initial L forms ditemukan dan berperan sebagai jangkar perlekatan mikroorganisme lain yang berkolonisasi pada permukaan gigi dan menjadi bahan aktif yang memperburuk SAR pasien.…”

Section: Pendahuluanunclassified

Uji zona hambat ekstrak metanol teripang putih (holothuria scabra) mentawai terhadap Streptococcus sanguinis pada Stomatitis Aftosa Rekuren secara in vitro: studi eksperimental

Rahmah,

Arma,

Lestari

et al. 2024

Padjadjaran J Dent Res Students

View full text Add to dashboard Cite

ABSTRAKPendahuluan: Stomatitis aftosa rekuren (SAR) atau sariawan merupakan ulser berbentuk oval atau bulat yang sakit dan terjadi secara berulang, serta dapat sembuh secara spontan dengan atau tanpa pengobatan. Streptococcus sanguinis (S. sanguinis) bentuk initial L forms banyak ditemukan pada penderita SAR dan dapat memperparah kondisi SAR. Salah satu hewan yang banyak dimanfaatkan sebagai bahan untuk pengobatan alternatif adalah teripang putih atau Holothuria scabra (H. scabra) yang berasal dari kepulauan Mentawai. Tujuan penelitian ini adalah menganalisis daya hambat ekstrak metanol teripang putih (H. scabra) terhadap S. sanguinis yang banyak ditemukan pada penderita Stomatitis Aftosa Rekuren secara in vitro. Metode: Jenis penelitian eksperimental laboratoris dengan rancangan penelitian posttest only control group design. Sampel pada penelitian ini adalah ekstrak metanol teripang putih dari pulau Siberut, kepulauan Mentawai, yang diperoleh dalam keadaan sudah kering di kota Padang dan bakteri S. sanguinis ATCC 10556 yang diperoleh dari Laboratorium Penelitian Antar Universitas (PAU) Universitas Gadjah Mada, Yogyakarta. Terdapat 5 kelompok perlakuan, yaitu pengujian dengan konsentrasi ekstrak metanol teripang putih (H. scabra) 2, 4, dan 8%, serta kontrol positif (chlorhexidine 0,2%) dan kontrol negatif. Masing-masing kelompok perlakuan diulang sebanyak 5 kali sehingga besar sampel menjadi total 25 pengulangan. Data yang diperoleh diolah menggunakan uji Independent Sample (T-Test). Hasil: Terdapat zona hambat ekstrak metanol teripang putih (H. scabra) terhadap S. sanguinis pada stomatitis aftosa rekuren yang diujikan secara in vitro pada konsentrasi 8% dengan sig 0,001 atau < 0,05, sebesar 0,81 mm, sedangkan pada konsentrasi 2 dan 4% tidak terdapat zona hambat. Simpulan: Ekstrak metanol teripang putih (H. scabra) Mentawai memiliki zona hambat pada konsentrasi 8%, tetapi dalam kategori lemah (weak) dan kurang efektif jika dibandingkan dengan kontrol positif yaitu chlorhexidine 0,2%KATA KUNCI: stomatitis aftosa rekuren, s. sanguinis, h. scabra, zona hambat, ekstrak teripangInhibition zone test of methanol extract of mentawai white sea cucumber (holothuria scabra) against streptococcus sanguinis in recurrent aphthous stomatitis in vitro: experimental studyABSTRACTIntroduction: Recurrent aphthous stomatitis (SAR) or canker sores are painful oval or round ulcers that occur recurrently and can heal spontaneously with or without treatment. Streptococcus sanguinis (S. sanguinis) plays a role in aggravating SAR in patients and mostly found in the of initial L forms. One of the animals that is widely used as an ingredient for alternative medicine is white sea cucumber or Holothuria scabra (H. scabra) from the Mentawai islands. The purpose of this study was to analyze the inhibition zone of white sea cucumber (H. scabra) methanol extract against S. sanguinis in recurrent aphthous stomatitis (in vitro study). Methods: Laboratory experimental and posttest only control group designs were used in this study. The samples in this study were a dry form of methanol extract of white sea cucumber from Siberut island, Mentawai islands obtained in Padang city. The S. sanguinis ATCC 10556 bacteria obtained from the Inter-University Research Laboratory (PAU) of Gadjah Mada University, Yogyakarta. There were 5 treatment groups, namely 2, 4, and 8%, of white sea cucumber (H. scabra) methanol extract, as well as positive control (0.2% chlorhexidine) and negative control. Each treatment group was repeated 5 times so that the sample size was a total of 25 repetitions. The data obtained was processed using the Independent Sample test (T-Test). Results: The obtained zone of inhibition of the 8% methanol extract of white sea cucumber (H. scabra) against S. sanguinis was 0.81 mm, with a significance of 0.001 (<0.05), while at concentrations of 2% and 4% there were no zone of inhibition. Conclusion: Methanol extract of Mentawai white sea cucumber (H. scabra) has an inhibition zone at the best concentration of 8%, with a weak category against S. sanguinis bacteria and less effective when compared to the 0.2% chlorhexidine.KEY WORDS: recurrent aphthous stomatitis, s. sanguinis, h. scabra, inhibition zone, sea cucumber extract.

show abstract

Section: Pendahuluanunclassified

Uji zona hambat ekstrak metanol teripang putih (holothuria scabra) mentawai terhadap Streptococcus sanguinis pada Stomatitis Aftosa Rekuren secara in vitro: studi eksperimental

Rahmah,

Arma,

Lestari

et al. 2024

Padjadjaran J Dent Res Students

View full text Add to dashboard Cite

show abstract

“…24 Tanin bekerja dengan melarutkan lapisan lemak dan mengikat protein pembentuk dinding sel. 25 Alkaloid mengganggu terbentuknya dinding peptidoglikan sel bakteri sehingga tidak terbentuk secara utuh dan menyebabkan kematian sel. 26 Senyawa steroid dalam interaksinya dengan fosfolipid sel juga dapat menyerang membran lipid sehingga terjadi kebocoran lisosom yang menurunkan integritas membran sel serta diikuti lisisnya sel.…”

Section: Pembahasanunclassified

Antibiofilm of Arumanis Mango Leaves (Mangifera indica L.) Ethanol Extract Against Staphylococcus aureus in vitro

Hepziba,

Soesanto,

Widyarman

2023

J Indones Dent Assoc

View full text Add to dashboard Cite

Introduction: Staphylococcus aureus is a pathogen bacteria that forms biofilm and induces acute inflammation associated with dentoalveolar abscess, which needs antibiotics such as amoxicillin for the treatment. Due to antibiotic resistance and various side effects, natural ingredients are needed as an alternative treatment. Arumanis mango leaves (Mangifera indica L.) contains mangiferin, flavonoid, and tannin which are expected to inhibit the growth of Staphylococcus aureus and its biofilm formation. Objective: The aim of this study was to determine the antibacterial and antibiofilm effects of Mangifera indica L. leaves against Staphylococcus aureus in vitro. Methods: This study was in vitro laboratory experiment with post test only control group design. Antibacterial test was carried out by plate count method and antibiofilm test was carried out by microtiter plate biofilm assay method. Staphylococcus aureus ATCC 25923 was cultured in BHI broth in 37oC for 24h with anaerobic condition. The present study used ethanol extract of Mangifera indica L. leaves in 6 different concentrations: 3,125%, 6,25%, 12,5%, 25%, 50%, and 100%. Amoxicillin was used as positive control and DMSO 10% used as negative control. Data was analyzed with Shapiro-wilk test, One-way ANOVA test, and post hoc Tukey HSD test with p<0,05 as the level of significance. Results: The most effective concentration to inhibit the growth of Staphylococcus aureus was 100% extract compared to negative control (p<0,05). The most effective concentration against Staphylococcus aureus biofilm formation was 100% extract in 3hr incubation compared to negative control (p<0,05). Conclusion: Ethanol extract of Mangifera indica L. leaves has been shown in vitro to have antibacterial and antibiofilm properties against Staphylococcus aureus.

show abstract

“…SAR yang diketahui sebagai aphtae atau canker sores adalah salah satu penyakit mulut yang sudah diketahui oleh masyarakat awam sebagai sariawan (Nakhil, 2019). Streptococcus sanguinis sering dikaitkan dengan Stomatitis Aftosa Rekuren (SAR) baik sebagai patogen secara langsung atau sebagai stimulus antigenik dan mengakibatkan infeksi sekunder sehingga menyebabkan penyembuhan luka pada SAR terhambat (Norhayati, 2019). Mikroba Candida albicans merupakan jamur oportunistik penyebab sariawan, lesi pada kulit dan kandidiasis (Kurniawan, 2009).…”

Section: ■ Pendahuluanunclassified

Pengaruh Variasi Konsentrasi Gelling Agent HEC dalam Sediaan Gel Sariawan Ekstrak Daun Sirih Hitam terhadap Sifat Fisik Gel

Libba

Prasetya

Putri

2020

Proc. Mul. Pharm. Conf.

View full text Add to dashboard Cite

Recurrent Aftose Stomatitis (RAS) or sprue is one of the most common diseases of the oral mucosa. One of the contributing factor is the infection of Streptococcus sanguinis and Candida albicans. Black betel leaf contains a group of secondary metabolites that are used as antimicrobials . Gel is a good conductive preparation on mucouse membran. One of the factors that influence the physical properties of gel is the gelling agent. The purpose of this study was to determine the effect of HEC variation as a gelling agents on the physical properties of black betel leaf extract gel. Black betel leaf extract was obtained by maceration with 70% ethanol solvent. The gel was formulated into four formula with variations in HEC consentration is 1%, 1,5%, 2% and 2,5%. The antimicrobial activity of the extracts used the diffusion method of the wells. Tests of physical properties used organoleptic tests, homogenity, pH, spreadability and viscosity. The results showed that black betel leaf extract at a concentration of 2.5% was able to inhibit Streptococcus sanguinis and Candida albicans. An increase in HEC concentration can cause increase in the viscosity and decrease in the spreadibility, but does not affect organoleptics, homogenity and pH of the preparation. The best formula produced is formula F3 with 2% HEC composition.

show abstract

ANTIBACTERIAL POTENTIAL OF KAPUL FRUIT SKIN (Baccaurea macrocarpa) ON Streptococcus sanguis

Cited by 5 publications

References 2 publications

Uji zona hambat ekstrak metanol teripang putih (holothuria scabra) mentawai terhadap Streptococcus sanguinis pada Stomatitis Aftosa Rekuren secara in vitro: studi eksperimental

Uji zona hambat ekstrak metanol teripang putih (holothuria scabra) mentawai terhadap Streptococcus sanguinis pada Stomatitis Aftosa Rekuren secara in vitro: studi eksperimental

Antibiofilm of Arumanis Mango Leaves (Mangifera indica L.) Ethanol Extract Against Staphylococcus aureus in vitro

Pengaruh Variasi Konsentrasi Gelling Agent HEC dalam Sediaan Gel Sariawan Ekstrak Daun Sirih Hitam terhadap Sifat Fisik Gel

Contact Info

Product

Resources

About