Antibacterial Screening of Anthocyanic and Proanthocyanic Fractions from Cranberry Juice

Leitão, Denise P. S.; Polizello, Ana Cristina Morseli; Ito, Izabel Yoko; Spadaro, Augusto César Cropanese

doi:10.1089/jmf.2005.8.36

Cited by 59 publications

(49 citation statements)

References 19 publications

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“…demineralization of the tooth structure as shown in the root surface colonization assay. Furthermore, the antibacterial effect of PA has been previously investigated against S. mutans strains and the results were negative [Leitao et al, 2005]. Although GE treatment tended to decrease collagen degradation after 72 h of treatment, it did not achieve a significant inhibition of root caries compared to that of the 'no treatment' group.…”

Section: Discussionmentioning

confidence: 99%

Effects of Natural Cross-Linkers on the Stability of Dentin Collagen and the Inhibition of Root Caries in vitro

Walter

Miguez

Arnold³

et al. 2008

Caries Res

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Purpose: To evaluate the effects of dentin collagen modifications induced by various cross-linkers on the stability of collagen matrix and the inhibition of root caries. Materials and Methods: The following cross-linkers were tested: 5% glutaraldehyde (GA), 0.5% proanthocyanidin (PA), 0.625% genipin (GE). In the first experiment, cross-linker-treated demineralized human root dentin was digested with bacterial collagenase, centrifuged, and the supernatants were subjected to amino acid analysis to determine collagen content. The residues were analyzed by SDS-PAGE and hydroxyproline analysis. In the second experiment, bovine root surfaces were conditioned with phosphoric acid, treated with the cross-linkers, incubated with Streptococcus mutans and Lactobacillus acidophilus for 1 week and the root caries inhibition was evaluated with confocal microscopy. Lastly, the ability of the bacteria to colonize the root surface was evaluated. In this experiment slabs of bovine root were treated with the cross-linkers and incubated in a suspension of S. mutans and L. acidophilus. The slabs were washed, resuspended in water, glucose was added, and the pH measured. Results: While all collagen was digested with collagenase in the control groups, only a small proportion was solubilized in the GA-, PA-, and GE-treated groups. The root caries was significantly inhibited by treatment with PA or GA. Drops in pH in the cross-linker-treated groups were essentially the same as in the untreated group. Conclusion: Naturally occurring cross-linkers, especially PA, could be used to modify root dentin collagen to efficiently stabilize collagen and to increase its resistance against caries.

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Section: Discussionmentioning

confidence: 99%

Effects of Natural Cross-Linkers on the Stability of Dentin Collagen and the Inhibition of Root Caries in vitro

Walter

Miguez

Arnold³

et al. 2008

Caries Res

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“…Cranberry juice had at first been assumed to halt bacterial growth by acidifying the urine [1][2][3] or to harbor bactericidal/bacteriostatic compounds, 4,5 but studies failed to support these concepts. Instead, a mainstream of research has been committed on characterizing the newly detected anti-adhesive properties of cranberry products.…”

Section: Anti-adhesion Effects As Putative Anti-infective Mechanismmentioning

confidence: 99%

Cranberry products in the prevention of urinary tract infections: examining the evidence

Nowack¹,

Birck²

2015

BTAT

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Cranberry (Vaccinium macrocarpon) juice and extracts are widely used and recommended as folk remedy for prophylaxis of urinary tract infections (UTIs). Its putative mechanism is an anti-adhesive effect that prevents docking of bacteria on host tissues. The antiadhesion quality is attributed to A-type proanthocyanidins (PACs), a group of polyphenols that has a restricted occurrence in cranberries and a few related plants. Clinical trials with cranberry have provided a mixed evidence on behalf of UTI prophylaxis. In some trials, a benefit could not be detected due to lower than calculated UTI recurrence rates, in others failure had retrospectively been blamed on underdosing of cranberry products. To circumvent such problems, cranberry products need to be standardized for the bioactive principle of PAC and administered at a sufficient dose. Further characterization of PAC bioavailability, improvement of the currently inconvenient prescriptions, and above all of the palatability for patients is strongly recommended. Larger staged trials should then be carried out in patients with relevant UTI risks.

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“…There are references of antivirus activity (Konowalchuk & Speirs, 1978), antifungal activity (Cipollini & Stiles, 1992;Marwan & Nagel, 1986) and antibacterial activity (Lee et al, 2000;Leitão et al 2005;Marwan & Nagel, 1986). The last author reports no activity of cranberry juice (pH-3, 5 and 6, 9) and cranberry proanthocyanidin-rich fractions against E. coli and other bacteria.…”

Section: In Vitro and In Vivo Antimicrobial Activitymentioning

confidence: 99%

Antiadhesive Effect of Plant Compounds in Bacteria

Guirado¹,

Barreto²

2012

Phytochemicals as Nutraceuticals - Global Approaches to Their Role in Nutrition and Health

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Antibacterial Screening of Anthocyanic and Proanthocyanic Fractions from Cranberry Juice

Cited by 59 publications

References 19 publications

Effects of Natural Cross-Linkers on the Stability of Dentin Collagen and the Inhibition of Root Caries in vitro

Effects of Natural Cross-Linkers on the Stability of Dentin Collagen and the Inhibition of Root Caries in vitro

Cranberry products in the prevention of urinary tract infections: examining the evidence

Antiadhesive Effect of Plant Compounds in Bacteria

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