Antibacterial secondary metabolites from an endophytic fungus, <i>Arthrinium</i> sp. MFLUCC16-1053 isolated from <i>Zingiber cassumunar</i>

Pansanit, Acharavadee; Pripdeevech, Patcharee

doi:10.1080/21501203.2018.1481154

Cited by 38 publications

(44 citation statements)

References 24 publications

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“…e extract resuspended in chloroform showed zones of inhibition ranging from 12 to 17 mm with the most significant inhibition observed on B. subtilis (Table 2A; supplementary data). ese results were in correlation to previous studies conducted by Pansanit and Pripdeevech [55] and Salehan et al [56], where ethyl acetate extracts of endophytes from Zingiber cassumunar proved to be the most effective inhibitors of fungal growth, Gram-positive and negative bacteria [55,56]. is is also consistent with the various literature reports, showing that ethyl ether and ethyl acetate, are hydrogen bond acceptor molecules and can, therefore, extract electron donor solutes better than chloroform [57].…”

Section: Antimicrobial Activity and Micsupporting

confidence: 91%

Antimicrobial and Antioxidant Properties of a Bacterial Endophyte, Methylobacterium radiotolerans MAMP 4754, Isolated from Combretum erythrophyllum Seeds

Photolo

Mavumengwana

Sitole

et al. 2020

International Journal of Microbiology

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is study reports on the isolation and identification of Methylobacterium radiotolerans MAMP 4754 from the seeds of the medicinal plant, Combretum erythrophyllum, for the purposes of investigating antimicrobial and antioxidant activities from this endophyte. e strain identity was confirmed by 16S rRNA-based phylogeny and Scanning Electron Microscopy (SEM). Ethyl acetate and chloroform (1 : 1 v/v) extracts from the endophyte were tested for antimicrobial and antioxidant activity on a total of 7 bacterial species (3 Gram-positive and 4 Gram-negative) using the standard Minimum Inhibitory Concentration (MIC) protocol and Quantitative Radical Scavenging activity using the 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assay, respectively. e MICs were recorded at 250 μg/mL for B. subtilis ATCC 19659, B. cereus ATCC 1076, E. coli ATCC1053, and 62.5 μg/mL for K. oxytoca ATCC 13182 and M. smegmatis ATCC 21293, while an IC 50 of 5.65 μg/mL was recorded with the DPPH assay. Qualitative phytochemical analysis was positive for alkaloids, flavonoids, and steroids. Gas chromatography/mass spectrometry (GC/MS) analysis revealed the presence of 9-octadecene, 2,4-dinitrophenyl acetate, and 2(5H)-furanone, which have been previously reported for the targeted activities. M. radiotolerans MAMP 4754 tested positive for antimicrobial and antioxidant activity and this is linked to the production of plant-derived secondary metabolites by this strain. with only 3% of these being antimicrobial [4]. e pharmaceutical properties of medicinal plants have been linked to the production of a wide variety of structurally diverse phytochemicals which include alkaloids, flavonoids, terpenes, steroids, curcumins, saponins, and phenolics, all of which can potentially serve as drug lead candidates for the development of antimicrobials and resistance modifiers [5,6].According to the International Union for Conservation of Nature and the World Wildlife, there are an estimated 80 000 recognized medicinal flowering plant species globally,

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Section: Antimicrobial Activity and Micsupporting

confidence: 91%

Antimicrobial and Antioxidant Properties of a Bacterial Endophyte, Methylobacterium radiotolerans MAMP 4754, Isolated from Combretum erythrophyllum Seeds

Photolo

Mavumengwana

Sitole

et al. 2020

International Journal of Microbiology

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“…In comparison to free living fungi, crude extracts of fungal endophytes are an underexplored but rich source of bioactive and chemically diverse secondary metabolites which include terpenoids, alkaloids, phenols, furandiones, dimeric anthrones and benzopyroanones [4, 5]. This is evidenced by a detailed review of 46 genera and 111 species of fungal endophytes producing cytotoxic secondary metabolites by Chen et al [6].…”

Section: Introductionmentioning

confidence: 99%

Cytotoxic activity of crude extracts from Datura stramonium’s fungal endophytes against A549 lung carcinoma and UMG87 glioblastoma cell lines and LC-QTOF-MS/MS based metabolite profiling

Tapfuma

Uche-Okereafor

Sebola

et al. 2019

BMC Complement Altern Med

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BackgroundEndophytic fungi are a proven source of bioactive secondary metabolites that may provide lead compounds for novel drug discovery. In this study, crude extracts from fungal endophytes isolated from Datura stramonium were evaluated for cytotoxic activity on two human cancer cell lines.MethodsFungal endophytes were isolated from surface sterilized aerial parts of D. stramonium and identified using molecular, morphological and phylogenetic methods. Ethyl acetate crude extracts from these isolates were evaluated for cytotoxic activity on A549 lung carcinoma and UMG87 glioblastoma cell lines. Metabolite profiling was then performed by liquid chromatography coupled to quadrupole time-of-flight with tandem mass spectrometry (LC-QTOF-MS/MS) for the cytotoxic crude extract.ResultsEleven fungal endophytes were identified from D. stramonium. Significant cytotoxicity was only observed from the crude extract of Alternaria sp. KTDL7 on UMG87 glioblastoma cells (IC50 = 21.49 μg/ml). Metabolite profiling of this crude extract tentatively revealed the presence of the following secondary metabolites: 1,8-dihydroxynaphthalene (1), anserinone B (2), phelligridin B (3), metacytofilin (4), phomopsidin (5) and vermixocin A (6). Compounds 2 and 3 have been shown to be cytotoxic in literature.ConclusionThe findings in this study suggest that the crude extract of Alternaria sp. KTDL7 possesses compound(s) cytotoxic to glioblastoma multiforme cells. Future studies to isolate and characterize the cytotoxic compound(s) from this fungus could result in lead development of a fungal-based drug for glioblastoma multiforme treatment.

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“…The mycelia layer was sequentially macerated in methanol at room temperature for 2 days and followed by dichloromethane. The mycelia methanol and dichloromethane extracts were combined and further extracted three times with ethyl acetate (Pansanit and Pripdeevech 2018 ) to provide crude mycelia extracts (CME).…”

Section: Methodsmentioning

confidence: 99%

Cytotoxic endophyte from Camellia oleifera Abel - Penicillium chermesinum NR121310

2019

Self Cite

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Ten unknown endophytes were isolated from several parts of Camellia oleifera Abel and their biological activities were studied. One endophyte, Tea2-L1, showed the highest inhibition in various antioxidant capacity assay and cytotoxicity assays. The phylogenetic study suggested that Tea2-L1 endophyte is identified as Penicillium chermesinum NR121310 species. This shows the importance of the Penicillium species as a potential drug source.

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Antibacterial secondary metabolites from an endophytic fungus, Arthrinium sp. MFLUCC16-1053 isolated from Zingiber cassumunar

Cited by 38 publications

References 24 publications

Antimicrobial and Antioxidant Properties of a Bacterial Endophyte, Methylobacterium radiotolerans MAMP 4754, Isolated from Combretum erythrophyllum Seeds

Antimicrobial and Antioxidant Properties of a Bacterial Endophyte, Methylobacterium radiotolerans MAMP 4754, Isolated from Combretum erythrophyllum Seeds

Cytotoxic activity of crude extracts from Datura stramonium’s fungal endophytes against A549 lung carcinoma and UMG87 glioblastoma cell lines and LC-QTOF-MS/MS based metabolite profiling

Cytotoxic endophyte from Camellia oleifera Abel - Penicillium chermesinum NR121310

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